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A detection method for simultaneous acquisition of material components and total antioxidant activity in Lissae japonica

A technology of antioxidant activity and detection method, which is applied in the field of detection and analysis, can solve problems such as the inability to meet the quality control of traditional Chinese medicine, the lack of pharmacological activity research, and unclear interactions, and achieve the effects of easy promotion, simple and convenient operation, and high sensitivity

Active Publication Date: 2018-12-07
湖南省中医药研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Different from chemical drugs, traditional Chinese medicine and its related preparations have complex components, and the interaction between each substance is not clear
Therefore, the quality control standard through the determination of a single component cannot meet the quality control of traditional Chinese medicine.
After reviewing the literature of this patent, it is found that the evaluation of the quality of traditional Chinese medicine by means of chemical fingerprints often focuses on components such as high content and easy identification, but there is a lack of research on its pharmacological activity

Method used

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  • A detection method for simultaneous acquisition of material components and total antioxidant activity in Lissae japonica
  • A detection method for simultaneous acquisition of material components and total antioxidant activity in Lissae japonica
  • A detection method for simultaneous acquisition of material components and total antioxidant activity in Lissae japonica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A detection method for simultaneously obtaining material components (chlorogenic acid) and total antioxidant activity in Flos Japonica, comprising:

[0045] Preparation of the test solution: Accurately weigh 0.5g of the Jasmine Japonica sample into a 50mL Erlenmeyer flask, add 10mL of 60% ethanol, ultrasonicate at 65°C for 30min (power 300W), take 2.0mL of the supernatant in an EP tube, 10000r / Min centrifugation; precision pipette 1.0mL supernatant into a 25mL volumetric flask, constant volume, that is, the test solution.

[0046] Preparation of DPPH solution: Accurately weigh 2.0mg of DPPH standard substance, place it in a 50mL brown volumetric flask, add absolute ethanol to make up to the mark, shake well, store in dark place, and set aside.

[0047] Preparation of VC (vitamin C) standard solution: Accurately weigh 2.5mg of VC standard, place in a 50mL volumetric flask, add distilled water to the mark, shake well; accurately measure 2.5mL, 5.0mL, 7.5mL, 10.0mL standa...

experiment example 1

[0062] Orthogonal Test of Chlorogenic Acid Extraction Method in Experimental Example 1 Mountain Silver Flower

[0063] Taking extraction temperature (A), ethanol concentration (B), extraction time (C), and solid-liquid ratio (D) as independent variables, the experiment was designed as the response value of extraction rate of chlorogenic acid. The tests and results are shown in Table S1, Table S2 and Table S3.

[0064] Table S1 Orthogonal test factor levels for extraction process of chlorogenic acid from Jasmine Japonica

[0065]

[0066] Orthogonal test arrangement and results

[0067] Table S2 Experimental data

[0068]

[0069] Table S3 Analysis of variance

[0070]

[0071] From the results of the range analysis in Table S3, it can be seen that for the content of chlorogenic acid, the four factors affecting the extraction rate are in the order of C>B>A>D, and the optimal extraction condition is C2B2A2D1.

[0072] From the results of variance analysis in Table S...

experiment example 2

[0073] Experimental example 2 Determination of analysis conditions

[0074] 1) Preparation of DPPH ethanol solution:

[0075] Accurately weigh 2.0 mg DPPH, place it in a 50 mL brown volumetric flask, add absolute ethanol to the mark, shake well, and store in the dark to obtain a 0.04 mg / mL DPPH solution.

[0076] 2) Preparation of vitamin C series standard solutions:

[0077] Accurately weigh 2.5mg of VC standard product, place it in a 50mL volumetric flask, add distilled water to the mark, and shake well. Accurately measure 2.5mL, 5.0mL, 7.5mL, 10.0mL standard solutions, place them in 25mL volumetric flasks, add distilled water to dilute to the mark, and shake well for later use.

[0078] 3) Preparation of the test solution:

[0079] Accurately weigh 0.5g sample (passed through a No. 3 sieve) into a stoppered conical flask, add 10mL of 60% ethanol, ultrasonicate at 65°C for 30min, centrifuge at 10000r / min for 10min, take 1.0mL of the supernatant in a 25mL volumetric flask,...

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Abstract

The invention relates to a detection method for obtaining material composition and total antioxidant activity of lonicera confusa. The detection method comprises the following steps: preparing a test solution; preparing a DPPH solution; preparing a series of concentration gradient VC solution as a standard substance, carrying out HPLC-DPPH analysis by taking DPPH as a to-be-scavenged object, and establishing a regression equation of relationship between VC concentration and free radical scavenging rate; and injecting the test solution into the HPLC-DPPH analysis system to obtaining the chemical ingredient information of a sample and the total free radical scavenging rate of the sample, and substituting into the regression equation, so that the total antioxidant activity of the sample is obtained. The detection method provided by the invention has the advantages that an HPLC-DPPH online combination method is adopted, and chemical fingerprint chromatography information and the total antioxidant activity of the lonicera confusa can be obtained at the same time, so that the advantage of carrying out quality control and 'spectrum-effect' evaluation on the lonicera confusa is realized.

Description

technical field [0001] The invention relates to a method for simultaneously detecting chemical substance information and total antioxidant activity in Flos Japonica by HPLC-DPPH method, belonging to the technical field of detection and analysis. Background technique [0002] Mountain silver flower is the dry bud or the first opening of Lonicera macranthoides Hand.-Mazz., Lonicera hypoglauca Miq., Lonicera confusa DC. or Lonicera fulvotomentosa Hsu et S.C.Cheng flowers. It has the effect of clearing away heat and detoxifying, cooling wind and heat, and is commonly used in the treatment of carbuncle sores, sore throat, erysipelas, heat-toxin bloody dysentery, wind-heat cold, febrile disease and fever. It has antibacterial and antiviral effects, is a commonly used bulk medicinal material, and is widely used in clinical practice. However, the current research has not yet established an online method for testing the antioxidant activity of L. japonicus. Off-line step-by-step as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/89G01N30/74G01N30/14G01N30/86
CPCG01N30/14G01N30/74G01N30/8686G01N30/89
Inventor 张水寒黄建华周融融
Owner 湖南省中医药研究院
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