Quick antibacterial test method and kit

A test method and kit technology, applied in the field of microbiology test, can solve the problems of long test period, impact on quantification, poor control of the number of colonies in the negative control group, etc., and achieve the effect of saving test cost, less reagent volume and cost

Active Publication Date: 2015-01-21
承功(厦门)生物科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The test period of this standard method is relatively long, about 48 hours, and the number of colonies in the negative control group (without antibacterial agent) is not easy to control. Generally, the number of colonies in each negative control group is better between 100-900, too low or Too high will affect quantitative
Therefore, the current antibacterial (inhibition) test method is not suitable for the rapid detection of intermediate products or semi-finished products on the production line, nor is it suitable for high-throughput screening of a large number of samples

Method used

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  • Quick antibacterial test method and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Such as figure 1 , figure 2 Shown, a rapid antibacterial test kit, comprising:

[0041] (1) Sterile porous detection plate 1 (15 wells): made of transparent plastic molding, containing 3 rows and 5 columns of 15 detection holes 2 of equal size, the three columns on the left are sample wells, and the two columns on the right are control wells. The volume of each detection hole 2 is 0.1-0.5ml, and the bottom of the detection hole has uniform thickness and uniform texture.

[0042] (2) Moisturizing and heat-preserving culture box (1): It is made of transparent plastic, consisting of a box body and a box cover. A sterile wet tissue is placed on the bottom, and the detection plate 1 is placed on the sterile wet tissue.

[0043] (3) Culture solution (30ml): a bottle of LB culture solution.

[0044] (4) Test bacteria (1 bottle): Escherichia coli (ATCC 25922) grows on the surface of the LB agar medium on the slope.

[0045] (5) Positive control antibacterial agent (1 ml): ...

Embodiment 2

[0047] A preparation method of a rapid antibacterial test kit, comprising the steps of:

[0048] (1) Preparation of sterile multi-well test plate 1: press figure 2 Molding as shown is then made with transparent plastic casting by known methods. The test plate 1 consists of 15 test holes 2 in 3 rows and 5 columns. The 3X3 holes on the left (three groups of S1, S2 and S3) are sample holes for detecting antibacterial samples; the 2X3 holes on the right (two groups of D1 and D2) ) is a control well, which is used to detect positive control and negative control antibacterial agents; the volume of each well is about 0.35ml; it is sealed and packaged in a sterile plastic bag for subsequent use.

[0049] (2) Preparation of culture solution: LB culture solution was prepared and divided into 50ml glass bottles, 30ml per bottle, sealed and stored after autoclaving, for future use.

[0050](3) Preparation of test bacteria: after high-pressure sterilization of LB agar medium, cool it to...

Embodiment 3

[0055] Compare the effect of OD470 and OD600 in detecting bacterial density:

[0056] Take 0.5ml of Escherichia coli (ATCC 25922) overnight bacteria solution, dilute it with 5ml of LB culture solution and culture it on a shaker at 37°C for 3 hours, take 0.1ml of the bacteria solution and mix it with 0.1ml of LB culture solution, and then use LB The culture solution was serially diluted 7 times, and the last time only the LB culture solution was free of bacteria, and then the serial serial dilution of the bacteria solution (8 parts) was transferred to the microplate plate, and detected at 470nm and 600nm respectively with a microplate reader The OD value of each group of bacteria solution after serial dilution, the results are shown in Table 1 and image 3 shown.

[0057] Table 1. The results of detection of OD450 and OD600 at different bacterial dilutions

[0058] Bacteria density

[0059] The test results prove that the bacterial density in the bacterial liquid ca...

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Abstract

The invention provides a quick antibacterial test method and a kit. The kit comprises an aseptic porous detection board, a moisturizing and heat preservation culture box, a culture solution, test organisms and a positive control antibacterial agent, wherein the aseptic porous detection board comprises three or more detection holes with the same size, transparent materials with the same thicknesses and uniform texture are arranged at the bottoms of the detection holes, the moisturizing and heat preservation culture box is composed of a box body and a box cover, 1-10 detection boards can be placed in the box body, the culture solution is a culture solution necessary for the growth of the test organisms. The quick antibacterial test method and the kit provided by the invention can be used for quickly and conveniently detecting the antibacterial ability of a test sample and have the advantages of high throughput and low cost.

Description

technical field [0001] The invention belongs to the field of microbial tests, in particular to a rapid antibacterial test method and a kit. Background technique [0002] Antibacterial test is a test method used to detect and evaluate the antibacterial effect of antibacterial agents. It can also be used to screen new antibacterial drugs and detect bacterial resistance. [0003] The current standard antibacterial test method is based on the calculation of the number of colonies on the agar plate to determine the performance of the antibacterial agent, and the test period is relatively long. The operation steps of the standard anti-bacteria product anti-bacteria performance test method are as follows: [0004] Culture the test bacteria (Staphylococcus aureus: ATCC 6538 or Escherichia coli: 8099 or ATCC 25922) on a slant for 24 hours, wash the culture with PBS, and make a bacterial suspension. The required concentration is: drop 100 μl on 5ml control sample In the liquid (50X ...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12Q1/18
CPCC12M23/12C12M37/06C12Q1/18
Inventor 邹潮
Owner 承功(厦门)生物科技有限公司
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