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Rapid breeding method of tree seedlings of idesia polycarpa maxim. var. vestita diels

A technology of saplings and saplings, applied in the field of biological reproduction, can solve the problems of high requirements for female parent materials, seasonal constraints, and slow reproduction speed, and achieve the effects of low labor intensity, overcoming large differentiation, and fast reproduction speed

Inactive Publication Date: 2017-09-05
四川森迪科技发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is: provide a kind of rapid breeding method of Jatropha jatropha saplings, solve the problem that the propagation speed of Jatropha jatropha in the prior art is slow, requires high female parent materials, is restricted by seasons, and has high labor intensity , the problem of high production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of rapid propagation method of Jatropha tomentosa sapling, the method comprises the following steps:

[0025] 1) selection and processing of explant: in spring, select 1-year-old semi-lignified branch, after described branch is sterilized, it is pruned into the explant that band 1 axillary bud, length are 1cm;

[0026] 2) Primary culture: place the explants obtained in step 1 in the induction medium for primary culture, and directly induce the culture to produce cluster buds; the induction medium is MS solid medium with 1.0mg / L of 6-BA, 0.3mg / L of NAA and 1.2mg / L of 2,4-dichlorophenoxyacetic acid; culture conditions: temperature 26°C, relative humidity 80%, light time 10 hours / day, light intensity 5500Lx;

[0027] 3) subculture: when the cluster buds produced in step 2 grow to 1.8cm, they are placed in the subculture medium for proliferation; the subculture medium is MS solid medium with 0.4mg / L The NAA of 6-BA, 0.2mg / L, KNO 3 , NH 4 NO 3 , CaCl 2 The conte...

Embodiment 2

[0032] A kind of rapid propagation method of Jatropha tomentosa sapling, the method comprises the following steps:

[0033] 1) selection and processing of explant: in spring, select 1-year-old semi-lignified branch, after described branch is sterilized, it is pruned into the explant that band 2 axillary buds, length are 2cm;

[0034] 2) Primary culture: place the explants obtained in step 1 in the induction medium for primary culture, and directly induce the culture to produce cluster buds; the induction medium is MS solid medium with 1.5mg / L of 6-BA, 0.8mg / L of NAA and 0.8mg / L of 2,4-dichlorophenoxyacetic acid; culture conditions: temperature 29°C, relative humidity 90%, light time 8 hours / day, light intensity 3500Lx;

[0035] 3) Subculture: when the cluster buds produced in step 2 grow to 1.8-2.4cm, they are placed in the subculture medium for proliferation culture; the subculture medium is MS solid medium with 0.24mg / The 6-BA of L, the NAA of 0.2mg / L, KNO in the subcultu...

Embodiment 3

[0040] A kind of rapid propagation method of Jatropha tomentosa sapling, the method comprises the following steps:

[0041]1) selection and processing of explant: in spring, select 1-year-old semi-lignified branch, after described branch is sterilized, it is pruned into the explant that band 2 axillary buds, length are 2cm;

[0042] 2) Primary culture: place the explants obtained in step 1 in the induction medium for primary culture, and directly induce the culture to produce cluster buds; the induction medium is MS solid medium with 1.2mg / L of 6-BA, 0.6mg / L of NAA and 1.0mg / L of 2,4-dichlorophenoxyacetic acid; culture conditions: temperature 27°C, relative humidity 85%, light time 9 hours / day, light intensity 4000Lx;

[0043] 3) subculture: when the cluster buds produced in step 2 grow to 2.0 cm, they are placed in the subculture medium for proliferation; the subculture medium is MS solid medium with 0.3 mg / L of The NAA of 6-BA, 0.3mg / L, KNO 3 , NH 4 NO 3 , CaCl 2 The c...

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Abstract

The invention discloses a rapid breeding method of Jatropha jatropha saplings, which solves the problem of slow breeding speed, high requirements on female parent materials, restricted by seasons, high labor intensity and high production cost in the prior art. question. The invention comprises the following steps: 1) selecting 1-year-old semi-lignified branches in spring, and pruning them into explants after disinfection; 2) placing the explants in an induction medium to induce and cultivate cluster buds; 3) cluster buds growing to 1.8-2.4cm for multiplication culture; 4) after 30-40 days of multiplication culture, cut cluster buds for rooting culture; 5) practice seedling training for tissue cultured seedlings after rooting culture; 6) transplant rooted sprouts after training Planted in seedling trays. The method of the invention is simple, easy to operate, fast in propagation speed, less demanding on female parent materials, less restricted by seasons, and beneficial to the industrialization and large-scale production of the tung tree clones.

Description

technical field [0001] The invention belongs to the technical field of biological reproduction, and in particular relates to a rapid breeding method for Jatropha jatropha saplings. Background technique [0002] Idesia polycarpa Maxim.var.vestita Diels is a deciduous tree belonging to the genus Idesia polycarpa Maxim.var.vestita Diels, which has high economic and ornamental value. Its wood is soft and can be used for construction, furniture, utensils, etc.; it is an excellent tree species for building fast-growing mixed forests and economic forests in mountains; it has many fragrant flowers and nectary glands, and is a honey source plant for beekeeping; the tree shape is beautiful, and the fruit is long. Preface, the fruits are numerous, the fruit color is vermilion, shaped like pearls, and it is an ornamental tree species in mountains and gardens; the oil content of Jatropha jatropha is 20%-30%, and the oil content of seeds is 20%-26%, which is a high-quality Excellent raw ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 孔利文杨志良赵思源伍超
Owner 四川森迪科技发展股份有限公司
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