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Preparation method of natural ajoene

A technology of ajoene and garlic, which is applied in the field of preparation of natural garlic ajoene, can solve the problems of low ajoene purity, complicated extraction process, and difficulty in industrialized production, and achieve strong practical value, safe and easy solvent The effect of gaining and simple process

Active Publication Date: 2017-09-01
深圳市阿霍烯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a preparation method of natural garlic ajoene, to solve the problems of the prior art that the purity of ajoene is not high, a large amount of organic solvents are used in the extraction process, the extraction process is complicated, and it is difficult to realize industrial production.

Method used

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  • Preparation method of natural ajoene
  • Preparation method of natural ajoene
  • Preparation method of natural ajoene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1, get the garlic 10Kg that produces from Shandong, squeeze the juice with juice extractor after pulverizing, get garlic juice, the yield of garlic juice is 30%.

[0052] 2. Add 3Kg of 0.5% sodium bicarbonate solution to the above garlic juice, mix well, place in a temperature-controlled fermenter, adjust the temperature to 30°C, and ferment for 2 days.

[0053] 3. After the mixture is fermented for 2 days, take the oil in the upper layer of the mixture, add 500g of olive oil, mix well, and set aside.

[0054] 4. Extract the above-mentioned olive oil mixture with 65% ethanol, extract 3 times, each 500mL, combine 3 times of 65% ethanol extracts. The extract is concentrated under reduced pressure at a temperature of 55-75° C. to remove ethanol in the extract to obtain ajoene oil.

[0055] 5. Take carbon eighteen bonded silica gel C 18 1Kg, packed into a glass column for chromatography, and ajoene oil was loaded dry.

[0056] 6. Take 5Kg of 30% ethanol to elute the chro...

Embodiment 2

[0060] 1, get the garlic 10Kg that produces from Gansu, squeeze the juice with juice extractor after pulverizing, get garlic juice, the yield of garlic juice is 30%.

[0061] 2. Add 6Kg of 0.1% sodium hydroxide solution to the above garlic juice, mix well, place in a temperature-controllable fermenter, adjust the temperature to 10°C, and ferment for 6 days.

[0062] 3. After the mixture has been fermented for 6 days, take the oil in the upper layer of the mixture, add 1000g of rapeseed oil, mix well, and set aside.

[0063] 4. Extract the above-mentioned rapeseed oil mixture with 1000 mL of 95% ethanol, and extract once. The extract is concentrated under reduced pressure at a temperature of 55-75° C. to remove ethanol in the extract to obtain ajoene oil.

[0064] 5. Take UniPS 10-3001Kg, put it into a glass column for chromatography, and load the ajoene oily substance in a dry method.

[0065] 6. Take 5Kg of 40% ethanol to elute the chromatographic column, the elution flow r...

Embodiment 3

[0069] 1, get the garlic 20Kg that produces from Jilin, squeeze the juice with juice extractor after pulverizing, get garlic juice, the yield of garlic juice is 30%.

[0070] 2. Add 1.2Kg of 0.2% dilute hydrochloric acid solution to the above garlic juice, mix well, place in a temperature-controlled fermenter, adjust the temperature to 50°C, and ferment for 1 day.

[0071] 3. After the mixture is fermented for 1 day, take the oil in the upper layer of the mixture, add 250g of linseed oil, mix well, and set aside.

[0072] 4. Extract the above-mentioned linseed oil mixture with 50% ethanol, extract 5 times, 250 mL each time, combine 5 times of 50% ethanol extracts. The extract is concentrated under reduced pressure at a temperature of 55-75° C. to remove ethanol in the extract to obtain ajoene oil.

[0073] 5. Take the macroporous resin AB-82Kg, put it into a glass column for chromatography, and load the ajoene oily substance in a dry method.

[0074] 6. Take 10Kg of 10% etha...

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Abstract

The invention discloses a preparation method of natural ajoene, which comprises the following steps: (1) breaking and juicing garlic powder, and separating to form a product A, (2) mixing the product A and a solvent for natural fermentation, and separating to form a product B, (3) mixing the product B and vegetable oil to form a product C, (4) performing solvent extraction on the product C to form an extract as a product D, (5) performing decompression concentration and column chromatography separation on the product D, eluting with a solution containing an eluent to form an eluent as a product E, and (6) performing decompression on the product E to remove the solvent, and obtaining a high-purity natural ajoene product. According to the method, allinase contained in garlic is subjected to fermentation, extraction, chromatography separation to form the high-purity natural ajoene sample; a technology is simple and stable; conversion of alliin into ajoene can be promoted; ajoene can be extracted and separated efficiently; the method has the advantages that the method is adapted industrial production, and the purity of ajoene is high.

Description

technical field [0001] The invention relates to the technical field of extraction and separation of traditional Chinese medicines, in particular to a preparation method of natural garlic ajoene. Background technique [0002] Garlic has been used as a herb and food for nearly two thousand years. Garlic has many pharmacological effects, such as sterilization, prevention and treatment of cardiovascular diseases, prevention and treatment of tumors and cancers, etc. It has been used as medicine and food in the folk for thousands of years. [0003] Garlic is rich in alliin, which mainly exists in the cytoplasm of garlic in a stable and odorless form. Alliin is the precursor substance of the main functional components in garlic. Alliinase (Alliinase) will quickly decompose alliin into thiosulfinate when cutting, mashing, chewing, etc., the main The compound is Allicin. Allicin and other thiosulfinates are very unstable at room temperature, and can be decomposed into sulfide form...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P11/00C07C319/28C07C323/65
CPCC07C319/28C12P11/00C07C323/65
Inventor 黄伟昌
Owner 深圳市阿霍烯生物科技有限公司
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