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Double-stimulation response drug based on EGCG and metal ions and preparation method

A stimuli-responsive, metal ion technology, applied in the direction of pharmaceutical formulations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., to achieve the effect of ensuring the safety of use, cheap raw materials, and mild conditions

Inactive Publication Date: 2017-08-29
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the preparation of drug carriers based on the coordination of EGCG and metal ions.

Method used

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  • Double-stimulation response drug based on EGCG and metal ions and preparation method
  • Double-stimulation response drug based on EGCG and metal ions and preparation method
  • Double-stimulation response drug based on EGCG and metal ions and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for preparing a hollow double-stimuli-responsive drug carrier based on EGCG and metal ions, comprising the following steps:

[0022] (1) Pour 30mL, 34.1mM dimethylimidazole aqueous solution into 4mL water containing polyvinylpyrrolidone and zinc nitrate, the concentration of the polyvinylpyrrolidone is 8.5mg / mL, the concentration of zinc nitrate is 0.8M, and stir for 10min , centrifuged, and washed twice with deionized water to obtain zeolite imidazole skeleton-8 particles;

[0023] (2) Disperse 40mg of zeolite imidazole skeleton-8 particles in 20mL deionized water, add 4mL, 24mM epigallocatechin gallate aqueous solution, shake to disperse evenly, add 2mL, 30mM ferric chloride aqueous solution, shake to make Disperse evenly, add 20 mL of 3-(N-morpholine) propanesulfonic acid sodium salt buffer solution with pH=7.8, shake for 1 min, centrifuge, wash the particles twice with deionized water and three times with EDTA aqueous solution with pH=8.0, 30 mL each time,...

Embodiment 2

[0026] The preparation method of the dual-stimuli-responsive drug based on EGCG and metal ions comprises the following steps:

[0027] (1) Add doxorubicin to 30mL, 68mM dimethylimidazole aqueous solution, make doxorubicin concentration be 3mg / mL, after dissolving, pour into 4mL water containing polyvinylpyrrolidone and zinc nitrate, the polyvinylpyrrolidone The concentration is 17 mg / mL, the concentration of zinc nitrate is 0.8M, stirred for 5 minutes, centrifuged, and washed twice with deionized water to obtain zeolite imidazole skeleton-8 particles containing doxorubicin;

[0028] (2) Disperse 40 mg of zeolite imidazole skeleton-8 particles containing doxorubicin in 40 mL of deionized water, add 1 mL, 30 mM epigallocatechin gallate aqueous solution, shake to disperse evenly, add 0.5 mL, 40 mM chlorine Alumina aqueous solution, oscillate to disperse evenly, add 40mL, pH=7 3-(N-morpholine)propanesulfonic acid sodium salt buffer solution, shake for 1min, centrifuge, wash the pa...

Embodiment 3

[0032] A preparation method based on EGCG and metal ion double stimulus response medicine, comprising the steps of:

[0033] (1) Add bovine serum albumin to 30mL, 3.4mM dimethylimidazole aqueous solution, make the concentration of water-soluble drug be 0.5mg / mL, after dissolving, pour into 4mL water containing polyvinylpyrrolidone and zinc nitrate, the polyethylene The concentration of pyrrolidone was 4mg / mL, the concentration of zinc nitrate was 0.2M, stirred for 60min, centrifuged, and washed with deionized water 4 times to obtain zeolite imidazole skeleton-8 particles containing bovine serum albumin;

[0034] (2) Disperse 40 mg of zeolite imidazole framework-8 particles containing bovine serum albumin in 10 mL of deionized water, add 6 mL, 8 mM epigallocatechin gallate aqueous solution, oscillate to disperse evenly, add 4 mL, 8 mM copper sulfate Aqueous solution, oscillate to disperse evenly, add 10mL, pH=8 3-(N-morpholine)propanesulfonic acid sodium salt buffer solution, s...

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Abstract

The invention discloses double-stimulation response drug based on EGCG and metal ions and a preparation method. The preparation method comprises the following steps: (1) adding water-soluble drug in a dimethyl imidazole aqueous solution, pouring the mixture in water which contains polyvinylpyrrolidone and zinc nitrate, stirring and centrifuging, and washing with deionized water to obtain zeolimidazole skeleton-8 particles which contains the water-soluble drug; and (2) dispersing the zeolimidazole skeleton-8 particles which contains the water-soluble drug in deionized water, adding an epigallocatechin gallate aqueous solution, vibrating to enable the components to be dispersed uniformly, adding a metal salt aqueous solution, vibrating to enable the components to be dispersed uniformly, adding a 3-(N-morpholine) sodium proparesulfonic acid salt buffer solution, vibrating, centrifuging, washing with water, washing with an EDTA aqueous solution, and washing with deionized water to obtain the double-stimulation response drug based on EGCG and metal ions. Preparation conditions are gentle, activity of the water-soluble drug and use safety of a carrier are guaranteed, raw materials are low in cost and easy to obtain, and the preparation process is simple.

Description

technical field [0001] The invention relates to a dual-stimuli-response drug based on EGCG and metal ions and a preparation method thereof, belonging to the fields of drugs, drug carriers and drug delivery. Background technique [0002] Tea contains a variety of effective ingredients that are beneficial to human health, such as tea polyphenols, tea polysaccharides, tea pigments, etc. Among them, the physiological activity and pharmacological functions of tea polyphenols are the most extensively studied. Epigallocatechin gallate (EGCG) is the most important tea polyphenol with various functions. EGCG has inhibitory effects on various stages of cancer, including enhancing the body's immune function, regulating the metabolism of carcinogens, inhibiting carcinogens, affecting the expression of oncogenes, and inducing apoptosis of various cancer cells. EGCG has a polyhydroxy structure, strong reducing ability, can effectively scavenge free radicals, and can protect normal cell D...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/22A61K47/02A61K31/704A61K38/38A61K38/28
CPCA61K9/5115A61K9/5123A61K31/704A61K38/28A61K38/385
Inventor 王晓莉杨菁孙洪范张超李轩领
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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