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Non-targeting multi-index electronic method for rapid detection of pesticide residues in edible agricultural products

A pesticide residue, non-target technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high price, valid period of only 2 or 3 years, food safety monitoring loopholes, etc., to achieve rapid detection, improve method efficiency, The effect of high reliability

Inactive Publication Date: 2017-08-22
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the actual work of pesticide residue laboratories, most laboratories do not store hundreds of pesticide standard products. The reason is that pesticide standard products are not only expensive, but also valid for only 2 or 3 years, requiring repeated investment.
There are only dozens of standard pesticides in general laboratories, and the types of pesticides that are routinely monitored are limited to these dozens, resulting in loopholes in food safety monitoring

Method used

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  • Non-targeting multi-index electronic method for rapid detection of pesticide residues in edible agricultural products
  • Non-targeting multi-index electronic method for rapid detection of pesticide residues in edible agricultural products
  • Non-targeting multi-index electronic method for rapid detection of pesticide residues in edible agricultural products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0045] More than 1,200 kinds of pesticides in celery (for specific pesticide types, please refer to the pesticides listed in the two patents with publication numbers CN105738460A and CN105628839A) LC-Q-TOF / MS and GC-Q-TOF / MS detection and confirmation technology implementation examples, Including the following steps;

[0046] 1. Specific steps of sample pretreatment technology:

[0047] 1.1 Take the edible part of the celery sample, chop it up, mix it evenly, seal it, and mark it;

[0048] 1.2 Weigh 10g of celery sample (accurate to 0.01g), add 40mL of 1% acetic acid acetonitrile into an 80mL centrifuge tube, use a high-speed homogenizer at 13500r / min, homogenize and extract for 1min, add 1g of sodium chloride, 4g of anhydrous sulfuric acid Magnesium, shake for 5min, centrifuge at 4200r / min for 5min, take 20mL of the supernatant, concentrate it to about 1mL by rotary evaporation in a water bath at 40°C, and wait for purification.

[0049] 1.3 Add about 2cm high anhydrous sod...

Embodiment 2

[0067] Implementation examples of LC-Q-TOF / MS and GC-Q-TOF / MS detection and confirmation techniques for more than 1,200 pesticides in tomato (such as the pesticides described above).

[0068] The sample pretreatment steps, LC-Q-TOF / MS and GC-Q-TOF / MS operating conditions, and the detection process of pesticide residues in samples are all referred to in Example 1.

[0069] LC-Q-TOF / MS and GC-Q-TOF / MS detection results in tomato samples in a provincial capital city: 19 commercially available tomato samples in a provincial capital city were collected, and LC-Q-TOF / MS and GC-Q-TOF were used LC-Q-TOF / MS and GC-Q-TOF / MS technologies detected 37 kinds of pesticide residues, with a total of 141 frequencies involving 19 samples. The specific results are shown in Table 2.

[0070] Table 2 Detection results of LC-Q-TOF / MS and GC-Q-TOF / MS in tomato samples from a certain area

[0071]

Embodiment 3

[0073] Implementation examples of LC-Q-TOF / MS and GC-Q-TOF / MS detection and confirmation techniques for more than 1,200 pesticides in grapes (such as the pesticides described above).

[0074] The sample pretreatment steps, LC-Q-TOF / MS and GC-Q-TOF / MS operating conditions, and the detection process of pesticide residues in samples are all referred to in Example 1.

[0075]LC-Q-TOF / MS and GC-Q-TOF / MS detection results in grape samples in a provincial capital city: 17 commercially available grape samples in a provincial capital city were collected, and LC-Q-TOF / MS and GC-Q-TOF were used LC-Q-TOF / MS and GC-Q-TOF / MS technologies detected 39 kinds of pesticide residues, with a total of 147 frequencies involving 16 samples. The specific results are shown in Table 3.

[0076] Table 3 Detection results of LC-Q-TOF / MS and GC-Q-TOF / MS in grape samples in a certain area

[0077]

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Abstract

The invention discloses a non-targeting multi-index electronic method for rapid detection of pesticide residues in edible agricultural products. The method comprises the following steps: 1, establishing a primary accurate mass spectrum database and a secondary fragment ion mass spectrum database of 1200 pesticides frequently used in the worldwide, applying the two mass spectrum databases to screening and confirmation of pesticide compounds and establishing a unique electronic identity card for each pesticide so as to replace real-pesticide standards used as references in traditional identification methods with electronic standards; 2, developing high-resolution-mass-spectrometry qualitative identification and intelligent screening software for automatic matching of mass spectra of pesticide residues and implanting a software program into an instrument so as to realize high-speed high-flux high-precision high-reliability high-informatization high-automation comparison between a test sample and the mass spectrum databases of the pesticides; and 3, carrying out sample preparation once and rapidly detecting 1200 or more pesticides at the same time by using two detection technologies. The method provided by the invention realizes multi-index non-targeting rapid detection of pesticide residues in edible agricultural products and is greatly improved in detection efficiency.

Description

technical field [0001] The present invention designs a method for detecting pesticide residues in edible agricultural products, in particular a non-target detection electronic method for simultaneous rapid detection of more than 1,200 pesticide residues in various edible agricultural products. Background technique [0002] As early as 1976, the World Health Organization (WHO), the Food and Agriculture Organization (FAO) and the United Nations Environment Program (UNEP) jointly established the Global Environment Monitoring System / Food Project (Global Environment Monitoring System, GEMS / Food), aiming to master member states The status of food contamination, understanding the intake of food contaminants, protecting human health, and promoting trade development. Now, all countries in the world have raised food safety to the strategic position of national security. Pesticide residue limit is one of the food safety standards and also the entry threshold for international trade. ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 庞国芳范春林常巧英邹小波
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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