Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Gamma-aminobutyric acid (GABA)-enriched mulberry leaf powder and preparation method thereof

A technology of aminobutyric acid and mulberry leaf powder, which is applied to the bacteria used in food preparation, the function of food ingredients, lactobacillus, etc., can solve the problem of limited GABA content in mulberry leaves, and achieve the effect of increasing the added value

Active Publication Date: 2017-08-18
武汉蜜欢健康产业发展有限公司
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional GABA enrichment of mulberry leaves mainly uses physical methods such as vacuum treatment or carbon dioxide treatment, but the above methods have been proved to be limited in improving the GABA content of mulberry leaves.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gamma-aminobutyric acid (GABA)-enriched mulberry leaf powder and preparation method thereof
  • Gamma-aminobutyric acid (GABA)-enriched mulberry leaf powder and preparation method thereof
  • Gamma-aminobutyric acid (GABA)-enriched mulberry leaf powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 A preparation method of mulberry leaf powder rich in γ-aminobutyric acid

[0047] A preparation method of mulberry leaf powder rich in gamma-aminobutyric acid, comprising the steps of:

[0048] S1. Fresh mulberry leaves are washed, dried, and crushed through a 1000-mesh sieve to obtain mulberry leaf powder; take mulberry leaf powder as the substrate, add 6% peptone accounting for the total weight of mulberry leaf powder, and 1.6% K 2 HPO 4 , 1.0% L-sodium glutamate and 60% water, after mixing, sterilize at 120°C for 20 minutes, cool for later use;

[0049] S2. Inoculate the activated Lactobacillus pentosus SS6 strain into the MRS liquid medium, and culture it statically at 37°C for 8 hours to obtain the seed liquid, and inoculate the seed liquid into the mixed mulberry leaf powder in step S1, the inoculation amount is 9% , and then fermented at 30°C for 36h;

[0050] S3. Drying and pulverizing the fermented mulberry leaf powder under vacuum conditions at 55...

Embodiment 2

[0051] Example 2 Optimization of the preparation process of mulberry leaf powder rich in γ-aminobutyric acid

[0052] 1. Optimization of carbon source addition in mixed mulberry leaf powder

[0053]S1. Fresh mulberry leaves were washed, dried, and crushed through a 1000-mesh sieve to obtain mulberry leaf powder; using mulberry leaf powder as a matrix, carbon sources (glucose, sucrose, xylose) were added according to 4 conditions, respectively 0%, 7%, 13%, 28% (w / w, the same below). Add 2% peptone as nitrogen source, add 0.42% K 2 HPO 4 , the initial water content is 60%;

[0054] S2. Inoculate the activated Lactobacillus pentosus SS6 strain into the MRS liquid medium, and cultivate it statically at 37°C for 8 hours as the seed liquid, inoculate the seed liquid into the mixed mulberry leaf powder in step S1, the inoculation amount is 5%, Ferment at 35°C for 36h.

[0055] S3. Drying and pulverizing the fermented mulberry leaf powder to obtain mulberry leaf powder rich in γ-...

Embodiment 3

[0064] K2HPO4 Addition Optimization in Mixed Mulberry Leaf Powder of Embodiment 3

[0065] 1. K in the mixed mulberry leaf powder 2 HPO 4 Addition optimization

[0066] S1. Fresh mulberry leaves are washed, dried, and crushed through a 1000-mesh sieve to obtain mulberry leaf powder; using mulberry leaf powder as the matrix, add K according to 8 conditions 2 HPO 4 , respectively 0%, 0.4%, 0.8%, 1.2%, 1.6%, 3.0%, 4.0%, 6.0% (w / w, the same below). Add 7% sucrose, add 6% peptone, and the initial water content is 60%;

[0067] S2. Inoculate the activated Lactobacillus pentosus SS6 strain into the MRS liquid medium, and cultivate it statically at 37°C for 8 hours as the seed liquid, inoculate the seed liquid into the mixed mulberry leaf powder in step S1, the inoculation amount is 5%, Ferment at 35°C for 36h.

[0068] S3. Drying and pulverizing the fermented mulberry leaf powder to obtain mulberry leaf powder rich in γ-aminobutyric acid.

[0069] 2. Results

[0070] The resu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses gamma-aminobutyric acid (GABA)-enriched mulberry leaf powder. A preparation method of the GABA-enriched mulberry leaf powder comprises the following steps of S1, preparation of mixed mulberry leaf powder: cleanly washing, drying and crushing a fresh mulberry leaf to subsequently obtain mulberry leaf powder, using the mulberry leaf powder as a matrix, adding, accounted for the total weight of the mulberry leaf powder, 0 to 14 percent of sucrose, 4 to 8 percent of peptone, 0.4 to 3 percent of L-sodium glutamate, 0.42 to 3 percent of dipotassium phosphate and 30 to 60 percent of water, uniformly mixing an obtained first mixture, and then carrying out sterilization; S2, fermentation culture: inoculating 5 to 9 percent of lactobacillus pentosus ss6 bacterial solution into the sterilized mixed mulberry leaf powder, and putting and culturing an obtained second mixture for 24h to 48h at 30 to 40 DEG C; S3, carrying out vacuum drying and crushing on the fermented mulberry leaf powder, so as to obtain the GABA enriched mulberry leaf powder. The mulberry leaf powder prepared by the preparation method is enriched in GABA having the content which can reach 54.95mg / g and which is 5 times of the content of the GABA in common mulberry leaf powder, moreover, is enriched in lactic acid bacteria, and has positive application significance in the fields of medicines and hygiene, health foods, feed and the like.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a mulberry leaf powder rich in gamma-aminobutyric acid and a preparation method thereof. Background technique [0002] Mulberry leaves are the leaves of Moraceae plants. Mulberry trees are widely planted in my country, with strong vitality and rich resources. The records of mulberry leaf as a dual-purpose resource of medicine and food such as "Shen Nong's Materia Medica", "Compendium of Materia Medica", and "The Pharmacopoeia of the People's Republic of China" all have detailed information. [0003] Since the 20th century, a large number of studies have been carried out on the active ingredients of mulberry and other medicines in my country and in the world, and their chemical structures, medicinal functions, etc., such as flavonoids, polysaccharides, sterols, alkaloids, etc., and their effects on the treatment of diabetes, The curative effect of hyperlipidemi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23L33/00A23L33/175A23L33/135A23K10/12A23K10/30A23K20/142
CPCA23V2002/00A23V2400/167A23V2200/30
Inventor 陈芳艳钟杨生吴珊林健荣
Owner 武汉蜜欢健康产业发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com