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Functional self-assembling nano-polypeptide hydrogel material and application thereof in preparation of hemostatic materials

A hydrogel and short peptide technology, applied in medical science, drug delivery, bandages, etc., can solve the problems of long hemostasis time, difficult dressing change, wound adhesion, etc., and achieves easy operation, good biocompatibility, and simple operation. Effect

Inactive Publication Date: 2017-08-15
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Commonly used clinical hemostatic materials such as hemostatic gauze, hemostatic fiber, and hemostatic bandage have limitations in use: the hemostatic time is longer, it is easy to adhere to the wound and it is difficult to change the dressing, and it is powerless to wound infection and suppuration
But a significant disadvantage is that the material aqueous solution has obvious acidity (pH=3~4), which will cause potential harm to the tissue of the hemostatic site

Method used

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  • Functional self-assembling nano-polypeptide hydrogel material and application thereof in preparation of hemostatic materials
  • Functional self-assembling nano-polypeptide hydrogel material and application thereof in preparation of hemostatic materials
  • Functional self-assembling nano-polypeptide hydrogel material and application thereof in preparation of hemostatic materials

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Effect test

Embodiment 1

[0029] Embodiment 1: the preparation of the polypeptide material of hydrogel

[0030] The short peptide (its sequence is: (arginine-alanine-aspartic acid-alanine) 4 -Arginine-Isoleucine-Lysine-Valine-Alanine-Valine) is dissolved in ultrapure water, and then configured into a solution with a concentration of 5~15ml / mL, and Tris-base solution Mix, the concentration of Trsi-base solution is 0.1~1 mol / mL, adjust its pH value to 7, and then mix with PBS / DMEM solution at a volume ratio of 2:1, and quickly form a gel after standing still, and after the properties are stable, Ready to use.

[0031] Such as figure 1 As shown, the rheometer was used to detect the shear thinning performance of the neutral aqueous solution of the above-mentioned short peptide. It has the characteristics of shear thinning and is very suitable for injection. It can be injected to the bleeding point to form a hydrogel instantly to control bleeding.

Embodiment 2

[0032] Embodiment 2: the preparation of the polypeptide material of hydrogel

[0033] The short peptide (its sequence is: (arginine-alanine-aspartic acid-alanine) 4 -Lysine-Isoleucine-Lysine-Valine-Alanine-Valine is dissolved in ultrapure water, and then configured into a solution with a concentration of 5~15ml / mL, and mixed with Tris-base solution , the concentration of Trsi-base solution is 0.1~1 mol / mL, adjust its pH value to 7, and then mix it with PBS / DMEM solution at a volume ratio of 2:1. ready for use.

Embodiment 3

[0034] Embodiment 3: the polypeptide material preparation of hydrogel

[0035] The short peptide (its sequence is: (arginine-alanine-aspartic acid-alanine) 4 -Histidine-Isoleucine-Lysine-Valine-Alanine-Valine is dissolved in ultrapure water, and then prepared into a solution with a concentration of 5~15ml / mL, and mixed with Tris-base solution , the concentration of Trsi-base solution is 0.1~1 mol / mL, adjust its pH value to 7, and then mix it with PBS / DMEM solution at a volume ratio of 2:1. ready for use.

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Abstract

The invention discloses a short peptide. The sequence of the short peptide is (arginine-alanine-aspartic acid-alanine)4-arginine-isoleucine-lysine-valine-alanine-valine, (arginine-alanine-aspartic acid-alanine)4-lysine-isoleucine-lysine-valine-alanine-valine or (arginine-alanine-aspartic acid-alanine)4-histidine-isoleucine-lysine-valine-alanine-valine. The invention simultaneously discloses a functional self-assembling nano-polypeptide hydrogel material prepared from the short peptide. The hydrogel material has a shear thinning characteristic, can immediately form hydrogel after being injected to a bleeding point through an injector, can be applied to rapid hemostasis and is particularly applied to the situation that the bleeding point environment is complicated; and the short peptide can form nano-fiber hydrogel at the room temperature when the pH value is neutral and can simulate a natural extracellular matrix structure and has good physical and chemical properties and biocompatibility.

Description

technical field [0001] The invention belongs to the technical field of hemostatic materials, and more specifically relates to a functional self-assembled nanometer polypeptide hydrogel material and its application in the preparation of hemostatic materials. Background technique [0002] First aid treatment of sudden accidents in daily life, trauma hemostasis in the operation process of patients in the hospital, especially the rescue of wounded soldiers in war, it is very important to effectively and quickly stop bleeding locally in patients, because uncontrolled bleeding is Leading cause of sudden accidents, hemorrhage during medical operations, or death on the battlefield. Early control of bleeding becomes the best strategy to reduce the death of patients. In the Iraq campaign, 50% of the casualties on the battlefield were due to excessive bleeding. Even when the casualty is able to support medical equipment for treatment, uncontrolled hemorrhage is still the leading fact...

Claims

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Application Information

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IPC IPC(8): A61L26/00
CPCA61L26/008A61L26/0047A61L2400/04C08L89/00
Inventor 何留民邵晗许小婷
Owner JINAN UNIVERSITY
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