Method for simultaneously determining four components in semen ziziphi spinosae by adopting accelerated solvent extraction-charged aerosol detector

Abstract

The invention discloses a method for simultaneously determining four components in semen ziziphi spinosae by adopting an accelerated solvent extraction-charged aerosol detector and aims at providing a method for simultaneously determining the four components in the semen ziziphi spinosae by adopting the accelerated solvent extraction-charged aerosol detector which is efficient, accurate and good in reproducibility. According to the technical main points, the method comprises the steps of (1) preparing a reference substance stock solution; (2) preparing a test sample solution; and (3) injecting a reference substance solution and the test sample solution into chromatograph for detection separately, wherein a chromatographic column is Thermo Synronis C18, a mobile phase is acetonitrile-water, the gradient elution program is 10-60% of acetonitrile and 90-40% of water for 0-8min, 60-80% of acetonitrile and 40-20% of water for 8-10, 80-100% of acetonitrile and 20-0% of water or 10-13min, and 100% of acetonitrile for 13-16min, the flow rate is 0.5mL.min<-1>, the column temperature is 40 DEG C, the atomization temperature is 35 DEG C and the sample size is 1muL. The method belongs to the technical field of chemical detection.

Description

technical field [0001] The invention relates to a method for simultaneously determining the contents of multiple components in Ziziphus jujube kernels, in particular to a method for simultaneously measuring four kinds of ingredients in Zizinus jujube kernels by rapid solvent extraction and an electric aerosol detector, and belongs to the technical field of chemical detection. Background technique [0002] Ziziphus jujuba Mill.var.Spinosa (Bunge) Huex H.F.Chou is the dry mature seed of Ziziphus jujuba Mill.var.Spinosa (Bunge) Huex H.F.Chou. It has the effects of nourishing the heart and nourishing the liver, calming the mind and calming the nerves, suppressing sweat, and promoting fluid production. It is often used for insomnia, palpitations and dreaminess, body deficiency and hyperhidrosis, and thirsty wounds. Its main effective chemical components are triterpenoid saponins, flavonoids, triterpenoids, alkaloids, etc. It has sedative and hypnotic, anti-anxiety, anti-convulsa...

AI Technical Summary

Problems solved by technology

For the determination of active ingredients in jujube seed, traditional methods mostly use liquid phase diode array detectors, evaporative light detectors and liquid phase mass spectrometry detection systems. [15-16] However, jujube saponins A, B and betulinic acid are triterpenoid saponins, and their ultraviolet absorption is poor. The sensitivity of diode array detector is low, while the detection baseline of evaporative light scatter detector (ELSD) is not stable enough, and the sensitivity is difficult to meet. Requirements: Although the liquid phase mass spectrometry detection system has high sensitivity, the instrument is expensive, complicated to operate, and difficult to maintain

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