A scanning electron microscope sample processing method of termite head sensory appendage

Abstract

The invention discloses a scanning electron microscope sample processing method for head sensory appendages of white ants. The method comprises the steps of putting white ants into a specially-made wind washing device, carrying out wind washing, feeding the white ants into a specially-made auxiliary vacuumizing device, carrying out vacuumizing operation to sink the white ants, carrying out front fixing and dissection, sequentially carrying out front rinsing under the view of a stereoscope and in an ultrasonic cleaner, carrying out rear fixing, rear rinsing and step-by-step gradient dehydration, putting the white ants into a specially-made filter paper cylinder, sealing the filter paper cylinder, putting the filter paper cylinder into a critical point drying frame, carrying out critical point drying, bonding samples close to a platform under the stereoscope, further dissecting target tissues of partial samples, carrying out secondary sample bonding, connecting the samples which are not subjected to secondary sample bonding with sample bonding adhesive tapes in a non-target observation region by virtue of a conductive silver adhesive, and carrying out all-direction gold sputtering after the conductive silver adhesive is dried. According to the scanning electron microscope sample processing method, multi-aspect technical problems in the background art that the surfaces of the samples are polluted, the samples are difficult to sink in fixation liquid and shrink to deform, different tissue parts of the samples are mutually shaded, and the samples are charged and discharged when being observed in an electron microscope are effectively solved, the cost for solving the problems is low, and the method has relatively high application value.

Description

technical field [0001] The invention relates to a biological sample processing method, in particular to a scanning electron microscope sample processing method for termite head sensory appendages. Background technique [0002] Termites are one of the five major pests in the world. They can cause damage to housing construction, greening and economic trees, reservoir dams, agricultural and forestry crops, places of interest, underground cables, ship bridges, cultural relics, archives, furniture and clothes, etc., not only causing serious economic losses, but also It poses a serious threat to people's lives. At present, there are nearly 3,000 species of termites known in the world, and nearly 500 species of termites are known in my country. In recent decades, with the continuous warming of the global climate, the harm of termites in my country has become increasingly serious, and the annual economic losses caused by termite damage are huge. [0003] The head of termites has im...

AI Technical Summary

Problems solved by technology

[0009] In order to overcome the shortcomings in the prior art, the present invention provides a scanning electron microscope sample processing method for the sensory appendages of the termite head, which effectively solves the problem of surface contamination of the sample, difficulty in sinking in the fixative, shrinkage of the sample and deformation of the sample. Various technical problems mentioned in the background technology, such as mutual occlusion of different parts of the tissue, charge and discharge of the sample when observed in the electron microscope, etc.

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