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Test paper for urine tyrosine

A technology for detecting test strips and tyrosine is applied in the field of urine tyrosine detection test strips, which can solve the problems of inaccurate, inconvenient, and unsafe urine tyrosine detection reagents, so as to avoid the pain of blood drawing and be safe to use. , the effect of low cost

Active Publication Date: 2017-08-04
HANYU GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the shortcomings of the existing urine tyrosine detection reagents that are inaccurate, unsafe, not environmentally friendly, and inconvenient, the purpose of the present invention is to provide a urine tyrosine detection test paper

Method used

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  • Test paper for urine tyrosine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The urine tyrosine detection test paper of the present invention comprises a support block, a tyrosine detection block, a vitamin C detection block and a color comparison card, and the tyrosine detection block and the vitamin C detection block use absorbent tissue paper as a carrier, and the length is 5mm, The width is 5mm and the thickness is 1mm.

[0038] The preparation process of the tyrosine detection block is as follows:

[0039] (1) Prepare a polyphenol oxidase solution with a concentration of 1000U / mL, put 1mm thick absorbent tissue paper into a container containing the prepared polyphenol oxidase solution, and after the absorbent tissue paper is fully infiltrated, take it out and freeze-dry it;

[0040] (2) Prepare an ascorbate oxidase solution with a concentration of 500U / mL, put the absorbent tissue paper treated in step (1) into a container filled with the prepared ascorbate oxidase solution, and after the absorbent tissue paper is fully infiltrated, take ou...

Embodiment 2

[0049] Prepare artificial urine quality control solution instead of normal urine, the formula is: distilled water, urea 1.8%, uric acid 0.05%, sodium chloride 1.1%, add tyrosine to the urine quality control solution, the final concentration is 3mmol / L.

[0050] Use the urine tyrosine detection test paper prepared in Example 1 to dip the artificial urine quality control solution, gently shake off the excess liquid, react in the air for 4 hours, and observe the color change.

[0051] Result: The vitamin C detection block is blue, the tyrosine detection block turns black, and the test result is positive for tyrosine.

Embodiment 3

[0053] Prepare artificial urine quality control solution instead of normal urine, the formula is: distilled water, urea 1.8%, uric acid 0.05%, sodium chloride 1.1%, add tyrosine to the urine quality control solution, the final concentration is 1.3mmol / L.

[0054] Use the urine tyrosine detection test paper prepared in Example 1 to dip the artificial urine quality control solution, gently shake off the excess liquid, react in the air for 4 hours, and observe the color change.

[0055] Result: The vitamin C test block is blue, the tyrosine test block turns gray, and the test result is negative for tyrosine.

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Abstract

The invention belongs to the technical field of test paper and particularly relates to a test paper for urine tyrosine. The test paper for urine tyrosine comprises a support block, a tyrosine detection block, a vitamin C detection block and a colourimetric card. The test paper has the advantages that (1) the tyrosine is catalyzed by using a biological compound enzyme to generate specific chromogenic reaction and is not disturbed by other substances of the urine except for vitamin C, so that the result is accurate; (2) the test paper is free of toxicity or corrosivity and is safe in use; (3) chemical substances contained in the test paper all are biodegradable and free of pollution to the environment; and (4) the dosage form is the test paper, so that the test paper is directly wetted by the urine during urination, so that the test paper is very convenient to use. In addition, sampling and operation are simple and fast during urine tyrosine testing, the pain of blood drawing and the opportunity of cross infection are eliminated, a special instrument is not needed and the cost is low, so that the test paper is suitable for popularization in cancer survey in primary hospitals and remote mountain areas.

Description

technical field [0001] The invention belongs to the technical field of detection test paper, in particular to a detection test paper for urine tyrosine. Background technique [0002] Worldwide, malignant tumors have surpassed other diseases and become the number one cause of death for humans, and the incidence is on the rise, seriously threatening human health. Commonly used methods for diagnosing tumors include chest X-ray, CT, MRI, puncture, and blood drawing. Among them, some methods use precise and expensive instruments, and some methods suffer from physical pain to the examinees, and even cross-infection and other problems may occur. Therefore, it is necessary to develop a detection method that is simple and quick to operate, low in cost, strong in specificity, high in sensitivity and good in repeatability. In recent years, more and more metabolomic studies have shown that body fluid metabolites can be used as markers for early detection or detection of various tumor ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/7759
Inventor 梁嘉亮赵泳婷罗勇
Owner HANYU GRP CO LTD
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