Method for rapidly propagating in vitro tissue of toona sinensis
A technology of Chinese toon and tissue, which is applied in the field of rapid propagation of Chinese toon isolated tissue, can solve the problems of time-consuming and laborious, long cycle, low breeding coefficient, etc., and achieve the effect of easy repetition, high differentiation rate and wide selection of materials
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Embodiment 1
[0063] 1. Culture medium preparation
[0064] 1) Seed germination medium: 1 / 2MS+0.5mg / L GA 3 +0.05mg / L NAA+0.3% sucrose+7g / L agar powder, pH 6.0;
[0065] 2) Callus induction medium: MS+0.15mg / L TDZ+0.3mg / L NAA+0.3% sucrose+7g / L agar powder, pH 6.0;
[0066] 3) Callus differentiation medium: MS+0.1mg / L 6-BA+0.5mg / L KT+0.1mg / L NAA+0.3% sucrose+7g / L agar powder, pH 6.0;
[0067] 4) Rooting medium: 1 / 2MS+0.5mg / L NAA+0.2% sucrose+6g / L agar powder, pH 6.0.
[0068] 2. Explant selection
[0069] 1) Aseptic seedling explants
[0070] Shell the Chinese toon seeds, remove the sundries, and wash them twice with water. The seeds were then soaked in a beaker and placed in a 25°C incubator (12h). Remove the floating seeds, wash the seeds in the lower layer, put them on a plate, cover them with wet gauze, and place them in a 25°C incubator (12h). Take a few seeds, put them in a petri dish, sterilize with 75% alcohol solution for 20 seconds, then sterilize with 0.1% mercuric chloride ...
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