Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Apocynum venetum polysaccharide, extraction method and application thereof

An extraction method and polysaccharide technology are applied in the field of traditional Chinese medicine extraction to achieve good anticoagulant effect.

Active Publication Date: 2017-07-28
HENAN UNIVERSITY
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pharmacological activity is closely related to the chemical composition, and there is no related report on the structure and in vitro thrombolytic (or fibrinolytic) effect of Apocynum polysaccharides. Therefore, the study of Apocynum polysaccharides is of great significance for the in-depth development and utilization of Apocynum

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Apocynum venetum polysaccharide, extraction method and application thereof
  • Apocynum venetum polysaccharide, extraction method and application thereof
  • Apocynum venetum polysaccharide, extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Extraction of polysaccharides from Apocynum apocynum

[0027] A method for extracting polysaccharides from Apocynum baicalensis, comprising the steps of:

[0028] 1) Take 770 g of shade-dried Apocynum bahua, and use 2 L of petroleum ether to reflux and extract twice at 60°C in a round-bottomed flask, each time for 1 h. After the reflux extraction, filter and dry the filter residue, add 9 L of 70% ethanol, heat extraction at 50 °C for 3 times, each heating extraction for 1 h. After heating and extraction, filter, and dry the filter residue to obtain 479.7 g of defatted apocynum twist. Take all the defatted Apocynum bahua, add 10 times the weight of distilled water and heat-extract at 90°C for 4 times, each time for 4 hours. After the hot extraction, the filtrate was concentrated under reduced pressure to obtain a concentrated solution. Then 95% ethanol was added to the concentrated solution to make the final concentration 70%. After standing for 24 h, the pre...

Embodiment 2

[0034] Example 2 Anticoagulant test of polysaccharides from Apocynum baicalensis.

[0035] METHODS: Four tests of plasma coagulation in rabbits in vitro.

[0036] Instruments and materials: TGL-16gR high-speed desktop refrigerated centrifuge (Shanghai Anting Scientific Instrument Factory); HF6000-4 semi-automatic coagulation analyzer (Southern Hanfang Medical Instrument Co., Ltd.); LRH-150 biochemical incubator (Shanghai Yiheng Technology Co., Ltd. company); sodium chloride injection; 0.109 mol / L sodium citrate solution, breviscapine for injection (Hunan Hengsheng Pharmaceutical Co., Ltd., 20110202); activated partial thrombin time (APTT) assay kit (1121911), coagulation Enzyme time (PT) assay kit (105295), thrombin time (TT) assay kit (121168), fibrinogen (FIB) content assay kit (132107) were all produced by Shanghai Sun Biotechnology Co., Ltd.

[0037] Experimental animals: Rex rabbit, male, weighing 2.0-2.5 kg, provided by the Institute of Traditional Chinese Medicine, Sch...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention relates to an extraction method of apocynum venetum polysaccharide. The extraction method comprises the following steps: 1) firstly degreasing apocynum venetum with petroleum ether, then extracting a fat-soluble ingredient with ethyl alcohol, taking filter residue and adding water at the temperature of 90+ / -5 DEG C for hot-extraction, carrying out alcohol precipitation after hot-extraction, taking sediment to wash with absolute ethyl alcohol, acetone and absolute ether in sequence, carrying out freeze drying, then dissolving with distilled water, adopting Sevag method to removal albumen, carrying out dialysis and freeze drying on an obtained supernate to obtain crude polysaccharide; and 2) after ion exchange chromatography and gel permeation column chromatography purification of the crude polysaccharide, and thus obtaining four polysaccharide components Vp1, Vp2a-I, Vp2a-II and Vp3. Test results indicate that the apocynum venetum refined polysaccharide component Vp1 has a good coagulation promotion effect, and can be used for preparing coagulation promotion drugs independently; and the apocynum venetum refined polysaccharide component Vp3 has a good coagulation promotion effect, and can be used for preparing anticoagulation drugs independently.

Description

technical field [0001] The invention belongs to the technical field of extracting traditional Chinese medicines, and in particular relates to polysaccharides from apocynum radish, an extraction method and applications thereof. Background technique [0002] Apocynum venetum (Apocynum venetum L) is a plant of the family Apocynaceae, commonly known as wild hemp, also known as "oleander hemp", "tea flower hemp", "tea tree seed" and so on. Apocynum hemp flower is sweet, bitter and slightly cold in nature and has the functions of calming the liver and lowering blood pressure, nourishing the heart and calming the nerves, clearing away heat and diuresis. Apocynum twist mainly contains flavonoids and cardiac glycosides, etc., and its active ingredients have the functions of lowering blood pressure and blood lipids, strengthening the heart and diuresis, calming the liver and soothing the liver, anti-inflammatory, relieving cough and asthma, and anti-allergy. In Xinjiang, local reside...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08B37/00A61P7/02A61P7/04
CPCC08B37/0003
Inventor 康文艺王丽丽王学标呼谧允王鹏禹
Owner HENAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com