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Application of mesenchymal stem cell culture or culture supernatant thereof

A kind of technology of stem cells and culture supernatant, which is applied in the field of preparing cosmetic compositions

Inactive Publication Date: 2017-07-28
北京泰盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there is no report on the use of mesenchymal stem cells, especially oral tissue-derived mesenchymal stem cells, for repairing skin tissue, slowing down skin aging, or preparing cosmetics.

Method used

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  • Application of mesenchymal stem cell culture or culture supernatant thereof
  • Application of mesenchymal stem cell culture or culture supernatant thereof
  • Application of mesenchymal stem cell culture or culture supernatant thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1 The cultivation of oral cavity-related stem cells

[0080] The cell culture medium used in this example is α-MEM medium without phenol red (product number: 41061-029).

[0081] 1. Cell passage method

[0082] Oral tissue stem cells to be primary extracted (see Example 4, including gingival stem cells, apical papilla stem cells, dental pulp stem cells, periodontal ligament stem cells, deciduous dental pulp stem cells and dental follicle precursor stem cells, etc.), cell growth fusion rate If it is more than 90%, it can be subcultured. The subculture method is a method commonly used in the art, specifically:

[0083] 1.1 Take out the cells to be treated from the incubator, observe their growth status under an inverted microscope, confirm that they are in the logarithmic growth phase, and then put them into the ultra-clean bench.

[0084] 1.2 After inserting the aspirator head into the yellow gun head, turn on the vacuum pump and suck up the supernatant.

[0...

Embodiment 2

[0095] Example 2 Stimulation of the culture supernatant of oral cavity-related stem cells on the growth and proliferation of other cells

[0096] 1. Brdu proliferation experiment:

[0097] According to the method in Example 1, the primary (i.e. zeroth generation) gingival stem cells and apical papilla stem cells were cultured to the third generation, and the cell supernatant after 2 days of culture was taken and transferred to the third generation of cells just seeded on a 6-well plate. Human periodontal ligament stem cells (PDLSC), jaw bone marrow stem cells (BMSC), and dental pulp stem cells (DPSC) were further cultured (the medium is phenol red-free medium, Cat. No. 41061-029, 37°C, 5% CO 2 ), each well contained 1ml cell suspension and 2ml cell supernatant transferred from gingival stem cells and apical papilla stem cells, and then detected the proliferation ratio of human periodontal ligament stem cells, jaw bone marrow stem cells, and dental pulp stem cells. Among them,...

Embodiment 3

[0129] Example 3 The effect experiment of the culture supernatant of oral cavity-related stem cells being used to improve the skin

[0130] 1) Lyophilization of cell culture supernatant

[0131] Take the culture supernatant of the fourth passage gingival stem cells. Transfer the culture supernatant to a new 150mm petri dish with a pipette, and store it in a freezer at -80°C for 24 hours or more, then put the frozen supernatant into a lyophilizer, and store it at -50°C Freeze-dried until the sample was dry powder, the results are shown in Figure 4 .

[0132] 2) Dissolution of cell culture supernatant lyophilized powder and mixing with cosmetic matrix

[0133] Get the freeze-dried powder in a 150mm petri dish after freeze-drying, add 5ml of serum to dissolve, then mix with 100g of cosmetic base, and said base is Cetaphil Shute skin moisturizing cream. The ingredients in Cetaphil Soothe Body Balm include: Water, Glyceryl Methacrylate (and) Propylene Glycol, Petrolatum, Dicap...

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Abstract

The invention relates to application of a mesenchymal stem cell culture or culture supernatant thereof. Specifically, the invention relates to application of the mesenchymal stem cell culture, the culture supernatant in the mesenchymal stem cell culture, secreta of mesenchymal stem cells, freeze-dried powder prepared from the culture, the culture supernatant or the secreta of mesenchymal stem cells and a solution prepared by dissolving the freeze-dried powder to preparation of cosmetic compositions. The invention also relates to cosmetic compositions containing TNF-alpha, IL-6 and IFN-gamma. The cosmetic compositions prepared in the invention can obviously improve skin, promote restoration of skin tissue and delay skin aging; and since mesenchymal stem cells are widely available and autologously applicable, the cosmetic compositions are safer and more convenient and have good application prospects.

Description

technical field [0001] The present invention relates to the use of mesenchymal stem cells, specifically, the present invention relates to mesenchymal stem cell cultures, culture supernatants in said mesenchymal stem cell cultures, secretions of mesenchymal stem cells, cultured or cultured The lyophilized powder prepared from the supernatant or the secretion of the mesenchymal stem cells and the solution obtained after the lyophilized powder is dissolved are used for preparing a cosmetic composition. The present invention also relates to cosmetic compositions containing TNF-alpha, IL-6 and IFN-gamma. Background technique [0002] Stem cells are a type of undifferentiated cells with self-replication ability and multi-directional differentiation potential, and have the potential function of regenerating various tissues and organs of the human body, so they are also called "universal cells" by the medical field. A number of studies have confirmed that stem cells can differentia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61K8/64A61Q19/02A61Q19/00A61Q19/08
CPCA61K8/64A61K8/981A61K2800/5922A61Q19/00A61Q19/02A61Q19/08
Inventor 施松涛
Owner 北京泰盛生物科技有限公司
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