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Method of preparing ferment by taking waste cordyceps militaris culture medium as raw material

A culture medium, Cordyceps militaris technology, applied in the directions of bacteria, natural extract-containing food ingredients, polysaccharide/gum-containing food ingredients, etc. used in food preparation

Inactive Publication Date: 2017-07-21
邓和满
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of method that takes waste Cordyceps militaris culture medium as raw material to prepare ferment, and this method can solve the problem of how to comprehensively utilize waste Cordyceps militaris culture medium as raw material preparation ferment method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] In this example, 1 kg of waste Cordyceps militaris culture medium was used as a raw material, crushed, 0.2 kg of wormwood leaves, 0.3 kg of honeysuckle, 0.2 kg of fungus, 0.3 kg of sea cabbage, 0.25 kg of tea, 0.2 kg of soybean meal, and 0.2 kg of artemisia annua were added. 0.3 kilograms of purple cabbage, then add 0.05 kilograms of chitosan, 0.02 kilograms of peptone, and 0.027 kilograms of mashed sweet potatoes, mix them evenly, add a composite strain composed of yeast, bifidobacterium and Aspergillus, and ferment at room temperature for 13 days; centrifuge and filter Then continue fermentation for 25 days to obtain an enzyme stock solution; then add 0.02 kg ~ 0.03 kg chitosan, 0.01 kg ~ 0.02 kg peptone, 0.03 kg sweet potato mash, fermentation at room temperature for 40 days, concentration, freeze drying, freeze drying temperature is- 40℃~-20℃.

[0015] The amount of yeast added in this example is 0.05% of the quality of the waste Cordyceps militaris culture, the additi...

Embodiment 2

[0017] In this example, 1 kg of waste Cordyceps militaris culture medium was used as raw material, crushed, 0.3 kg of wormwood leaves, 0.25 kg of honeysuckle, 0.3 kg of fungus, 0.25 kg of sea cabbage, 0.2 kg of tea, 0.25 kg of soybean meal, and 0.27 kg of artemisia annua were added. 0.2 kg of purple cabbage, then add 0.053 kg of chitosan, 0.01 kg of peptone, and 0.02 kg of mashed sweet potatoes, mix well, add a complex strain composed of yeast, bifidobacterium and Aspergillus, ferment at room temperature for 11 days; centrifuge and filter Then continue fermentation for 15 days to obtain an enzyme stock solution; then add 0.03 kg of chitosan, 0.01 kg ~ 0.02 kg of peptone, 0.02 kg of sweet potato mash, fermentation at room temperature for 47 days, concentration, freeze drying, freeze drying temperature is -40 ℃ ~ -20°C.

[0018] The amount of yeast added in this example is 0.02% of the quality of the waste Cordyceps militaris culture, the addition of bifidobacteria in this example ...

Embodiment 3

[0020] In this example, 1 kg of waste Cordyceps militaris culture medium was used as raw material, crushed, 0.25 kg of wormwood leaves, 0.2 kg of honeysuckle, 0.25 kg of fungus, 0.3 kg of sea cabbage, 0.3 kg of tea, 0.3 kg of soybean meal, and 0.3 kg of artemisia annua were added. 0.24 kilograms of purple cabbage, then add 0.06 kilograms of chitosan, 0.017 kilograms of peptone, and 0.03 kilograms of sweet potato mash, mix well, add a composite strain consisting of yeast, bifidobacterium and Aspergillus, and ferment for 10 days at room temperature; centrifuge and filter Then continue to ferment for 25 days to obtain an enzyme stock solution; then add 0.024 kg of chitosan, 0.01 kg to 0.02 kg of peptone, 0.02 kg to 0.03 kg of sweet potato mash, fermentation at room temperature for 60 days, concentration, freeze drying, freeze drying temperature- 40℃~-20℃.

[0021] The amount of yeast added in this example is 0.03% of the quality of the waste Cordyceps militaris culture, the addition...

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Abstract

The invention discloses a method of preparing a ferment by taking a waste cordyceps militaris culture medium as a raw material and relates to the technical field of cordyceps militaris preparation. The method takes the waste cordyceps militaris culture medium as the raw material and achieves comprehensive utilization of resources; folium artemisiae argyi, honeysuckle, fungus, sea lettuce, tea, soya bean meal, artemisia apiacea and purple cabbage are added into the waste cordyceps militaris culture medium; saccharomycete, bifidobacterium and aspergillus serve as a composite culture; and rich protease, lipase, superoxide dismutase, cellulase, amylase vitamins, polysaccharide substances and the like are generated.

Description

Technical field [0001] The invention relates to the technical field of enzyme preparation, in particular to a method for preparing an enzyme by using waste Cordyceps militaris culture medium as a raw material. Background technique [0002] Waste Cordyceps militaris culture medium often contains cordycepic acid and cordycepin. Cordycepic acid enters the second meridian of the lungs and kidneys, which can nourish lung yin and kidney yang, cure kidney deficiency, impotence and spermatorrhea, waist and knee pain, weakness after illness, chronic cough weakness It is the only Chinese medicine that can balance and regulate Yin and Yang at the same time. The cordycepin in Cordyceps militaris has antiviral, antibacterial, obvious inhibition of tumor growth, and interference with human RNA and DNA synthesis. However, after the harvest of the existing Cordyceps militaris, the culture medium is usually discarded, resulting in a waste of resources. Therefore, comprehensive The development an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/10A23L31/00A23L33/17A23L33/135A23L33/125A23L3/44
CPCA23L3/44A23V2002/00A23V2400/51A23V2250/208A23V2250/51A23V2250/21
Inventor 邓和满
Owner 邓和满
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