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UPLC determination method for polygala root

A detection method and technology of Polygala, applied in the field of UPLC determination of Polygala, can solve problems such as the influence of medicinal material quality, one-sidedness, complex chemical composition, etc.

Active Publication Date: 2017-07-04
TIANJIN TASLY MORDEN TCM RESOURCES
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Problems solved by technology

At the same time, due to the complex chemical composition of Polygala medicinal materials, it is relatively one-sided to control the quality of medicinal materials with only a few components, and other components in Polygala medicinal materials may also have an impact on the quality of medicinal materials

Method used

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  • UPLC determination method for polygala root
  • UPLC determination method for polygala root
  • UPLC determination method for polygala root

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Experimental program
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Embodiment

[0137] 1 Instruments and reagents

[0138] 1.1 Instrument Ultra-high performance liquid chromatography system (ACQUITY UPLC from Waters, including vacuum degasser, binary gradient pump, autosampler, column thermostat, TUV detector), ACQUITY UPLC-Synapt G2 MS chromatography-mass spectrometry Instrument (Waters Company), electronic balance (XS205, Swiss METTLER TOLEDO Company), high-speed refrigerated centrifuge (ST16R, American Thermo Company), Milli-Q ultrapure water system (American Millipore Company), ultrasonic cleaner (KQ-500DV, Kunshan Ultrasonic Instrument Co., Ltd.).

[0139] 1.2 Drugs and reagents sibiricose A5 (lot number: AP0590, purity 98%), sibiricose A6 (lot number: AP148S, purity 98%), sibiricaxanthone B (lot number AP046S, purity 98%), glomeratose A (lot number 20150701, purity 98%) were all Purchased from Tianjin Yifang Technology Co., Ltd., polygalaxanthone III (batch number 111850-201203, purity 95.7%) and 3,6'-disinapoyl sucrose (batch number 111848-201303,...

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Abstract

The invention relates to an UPLC determination method for a polygala root. The method comprises the following steps: step 1, preparation of a control solution: weighing sibiricose A5, sibiricose A6, sibiricaxanthone B, glomeratose A, polygalaxanthone III and 3,6'-disinapoyl sucrose, and fixing a volume by using methanol so as to prepare the mixed control solution; step 2, preparation of a test solution: extracting the medicinal material polygala root with water-containing lower alcohol and carrying out filtering so as to obtain the test solution; and step 3, determination: separately injecting the mixed control solution and the test solution into an ultra-high performance liquid chromatograph so as to obtain chromatograms and calculating the contents of active components in a test substance according to the chromatograms.

Description

[0001] Technical field: [0002] The invention relates to a detection method of Chinese medicinal materials, in particular to a UPLC detection method of polygala [0003] Background technique: [0004] Polygala is the dry root of Polygala.tenuifolia Willd. or Polygala.sibirica L., a plant of the genus Polygala in the family Polygala. It tastes bitter, pungent, and warm. The effect of swelling is used for insomnia, dreaminess, forgetfulness, palpitations, trance, etc. caused by heart-kidney disharmony. The quality of Polygala medicinal materials available in the market is uneven, the contents of polygalaxanthoneⅢ and 3,6'-disinapoyl sucrose are generally low, and the quality of medicinal materials is difficult to control. In recent years, most of the studies on the quality evaluation of polygala are based on the content determination and analysis of individual components as indicators. Jiang Yong and others carried out the research on the fingerprints of Polygala, but the dete...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 夏忠庭刘筱筱何毅宋兆辉
Owner TIANJIN TASLY MORDEN TCM RESOURCES
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