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Finger-print detection method for amino acid ingredients in Weikening tablet and application thereof

A fingerprint and Weikening tablet technology, which is applied in the field of detecting fingerprints of amino acid components in Weikening tablets and Weikening tablets

Active Publication Date: 2017-06-23
HEHUANG PHARMA SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of qualitative and quantitative research on Weikoning tablet system

Method used

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  • Finger-print detection method for amino acid ingredients in Weikening tablet and application thereof
  • Finger-print detection method for amino acid ingredients in Weikening tablet and application thereof
  • Finger-print detection method for amino acid ingredients in Weikening tablet and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] 1. Experimental part

[0110] 1.1 Sample pretreatment

[0111] Preparation of the test solution: Accurately weigh 2 g of Wei Ke Ning Tablets, place in a 100 mL Erlenmeyer flask, add 25 mL of 6mol / L hydrochloric acid to dissolve, then place in a water bath at 100±1°C for 8 hours, take it out to cool, and take the aqueous solution Put 10mL in an evaporating dish, concentrate to dryness in a water bath at 100±1°C, add 5mL of 0.1mol / L dilute hydrochloric acid to dissolve, filter through a needle filter with a 0.45μm filter membrane, and take the clear liquid for derivatization.

[0112] 1.2 Derivatization

[0113] Accurately measure 400 μL of the test solution, place it in a 2 mL centrifuge tube, add 200 μL of acetonitrile solution of 1mol / L triethylamine and 200 μL of acetonitrile solution of 0.1mol / L phenylisothiocyanate, shake well and let stand at room temperature to derivatize 1 Hour. After derivatization, add 800 μL of n-hexane, shake and stand for 10 min, take 400...

Embodiment 2

[0132] 1. Experimental part

[0133] 1.1 Sample pretreatment

[0134] Preparation of the test solution: accurately weigh a certain amount of Wei Ke Ning tablets, place in a 100mL Erlenmeyer flask, add 5-7mol / L hydrochloric acid to dissolve, the weight of Wei Ke Ning tablets added is the same as that of 5-7mol / L hydrochloric acid The volume ratio is 1:10-15 (g / ml). Then place it in a water bath at 100±5°C for 7-9 hours and take it out to cool. Take the aqueous solution and place it in an evaporating dish, concentrate it in a water bath at 100±5°C to dryness, and add the aqueous solution to 5-7mol / L hydrochloric acid at a volume ratio of 1:2-3. Then add 0.05-0.2mol / L dilute hydrochloric acid to dissolve, and the volume ratio of 0.05-0.2mol / L dilute hydrochloric acid to 5-7mol / L hydrochloric acid is 1:1-3. After filtering with a needle filter with a 0.45 μm filter membrane, the supernatant was taken and stored in a refrigerator at 4°C for derivatization.

[0135] 1.2 Derivati...

Embodiment 3

[0153] 1. Experimental part

[0154] 1.1 Sample pretreatment

[0155] Preparation of the testing solution: same as the preparation process of the testing solution in step 1.1 in Example 1.

[0156] 1.2 Pretreatment of reference substance

[0157] Preparation of reference substance solution: Accurately weigh 20 mg of glycine, arginine, alanine, leucine and phenylalanine reference substances respectively, dissolve with 0.1mol / L hydrochloric acid and constant volume, shake well, and gradually dilute , Obtain the reference substance solution from the reference substance stock solution. The contents of glycine, arginine, alanine, leucine, and phenylalanine in the reference solution were all 100 μg / ml.

[0158] 1.3 Derivatization

[0159] The derivatization process of the test solution is the same as that of the test solution in step 1.2 of Example 1.

[0160] Accurately measure 400 μL of the reference solution in step 1.2, place it in a 2 mL centrifuge tube, add 200 μL of acet...

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Abstract

The invention provides a finger-print detection method for a Weikening tablet. The method can obtain a finger print of the Weikening tablet by preparing, derivatizing and measuring test solution. The invention further provides a finger-print detection method for amino acid ingredients in the Weikening tablet. The finger-print detection method for amino acid ingredients in the Weikening tablet can obtain liquid chromatograms of the test solution, comparison product solution and blank contrast solution by preparing, derivatizing and measuring the test solution, the comparison product solution and the blank contrast solution, and the liquid chromatogram of the test solution is compared with liquid chromatograms of the comparison product solution and the blank contrast solution to obtain finger prints of the amino acid ingredients. The method also can serve as a measuring method for the content of the amino acid ingredients in the Weikening tablet and a quality detection method of the Weikening tablet. According to the method disclosed by the invention, measured results of the amino acid ingredients in the Weikening tablet are reliable; accuracy, repeatability and stability are good; the method also can serve as a quality control method of the Weikening tablet; multi-ingredient quantitative control on the quality of the Weikening tablet is achieved; the quality control level of the Weikening tablet is improved.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection of traditional Chinese medicine components, and relates to a method for detecting fingerprints of amino acid components in Weikening tablets and its application, in particular to a method for detecting fingerprints of amino acid components in Weikening tablets and Weikening tablets Detection method and its application in quality inspection. Background technique [0002] Weikening Tablets is based on the traditional ancient prescription "Ubei San", and is jointly produced by Shanghai No. 1 Traditional Chinese Medicine Pharmaceutical Factory (now Shanghai Hutchison Pharmaceutical Co., Ltd.) and Shanghai No. 1, Xiangshan, Renji, Zhongshan, Huashan and other hospitals. A drug for treating gastric diseases that has been clinically verified, controlled and successfully developed. It has been clinically proven that Weikoning Tablets have astringent, antacid and analgesic effects, and can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 沈丹萍姜鹏詹常森田禾苗
Owner HEHUANG PHARMA SHANGHAI
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