Liquid chromatography pre-column derivatization method for detecting glyphosate in water

Abstract

The invention discloses a liquid chromatography pre-column derivatization method for detecting glyphosate in water. The method comprises the steps as follows: S1, production of a standard curve; S2, preparation of a standard working solution; S3, preparation of a sample; S4, glyphosate derivatization; S5, online testing; S6, result determination; S7, quantitative measurement; S8, result calculation. With the adoption of the method, the glyphosate in water can be effectively detected and measured in the absence of a post-column derivatization instrument, a special detection chromatographic column and a post-column derivatization reagent, so that the laboratory cost is greatly saved, and the working efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of chromatographic detection and analysis, in particular to a liquid chromatography pre-column derivatization method for detecting glyphosate in water. Background technique [0002] Glyphosate (chemical formula: C 3 h 8 NO 5 P) is an effective active chemical component in many herbicides widely used. At present, the wastewater used to generate glyphosate is an acidic wastewater containing high concentrations of inorganic salts and high concentrations of organic matter, wherein the pollutants mainly include methanol and formaldehyde. , glyphosate, the COD mass concentration is about 48000-52000mg / L, and also contains about 15% of inorganic salts, such as sodium chloride (NaCl). Glyphosate dry powder and additives are sold as glyphosate aqueous solution with a mass fraction of 10%, but in this way, a large amount of inorganic salts and organic matter in the wastewater are brought to the farmland, which will...

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Problems solved by technology

[0002] Glyphosate (chemical formula: C 3 h 8 NO 5 P) is an effective active chemical component in many herbicides widely used. At present, the wastewater used to generate glyphosate is an acidic wastewater containing high concentrations of inorganic salts and high concentrations of organic matter, wherein the pollutants mainly include methanol and formaldehyde. , glyphosate, the COD mass concentration is about 48000-52000mg / L, and also contains about 15% of inorganic salts, such as sodium chloride (NaCl). Glyphosate dry powder and additives are sold as glyphosate aqueous solution with a mass fraction of 10%, but in this way, a large amount of inorganic salts and organic matter in the wastewater are brought to the farmland, which will further cause soil compaction and salinity Therefore, many researchers at home and abroad have carried out a lot of research work, mainly using physical and chemical methods, biological treatment methods, combined process methods and other glyphosate wastewater treatment technologies, but the existing glyphosate detection The method of phosphine is mostly carried out with the help of post-column derivatization instrument, special detection chromatographic column and post-column derivatization reagent, the detection cost is high, the procedure is complicated, and the efficiency is low

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