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Method for evaluating eye irritation by means of mouse kidney cell system

A system evaluation, renal cell technology, applied in the direction of biochemical equipment and methods, application, microbial determination/examination, etc., to achieve the effect of good correlation, clear genetic background, and difficult to differentiate

Active Publication Date: 2017-06-13
SOUTH CHINA INST OF COLLABORATIVE INNOVATION
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Cells used for in vitro testing include fibroblasts, blood cells, canine kidney cells, etc. Currently, there are no relevant patents and literature reports for in vitro methods based on standardized experimental animal mouse kidney cell systems

Method used

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  • Method for evaluating eye irritation by means of mouse kidney cell system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 1. Experimental materials

[0058] 1. Experimental reagent grade instruments

[0059] Fetal bovine serum (Gibco Company), the culture medium used in the examples refers to DMEM culture medium without phenol red and low sugar containing glutamine (Gibco Company), sodium fluorescein (Shanghai Aladdin Biochemical Technology Co., Ltd.), inserted cells Petri dishes (Millicell cell culture inserts from Corning, 24-well), collagenase I (Gibco), sodium chloride, potassium chloride, potassium hydrogen phosphate anhydrous, disodium hydrogen phosphate anhydrous, glucose, deionized Water, fluorescence spectrophotometer (Shanghai INESA-Analytical Instrument Factory, 970CRT);

[0060] 2. The test substance

[0061] According to the OECD database (Reference: Eye irritation. Reference chemicals databank (Second edition) [1998]), 5 classes of surfactants with known eye irritation classifications were selected, see Table 1 for details.

[0062] Table 1 List of test substances

[0063...

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Abstract

The invention discloses a method for evaluating eye irritation by means of a mouse kidney cell system, and the method is applicable to evaluation of cosmetic raw material irritation, in particular to evaluation of eye irritation of surfactants. According to the method, when irritation of a test material to cells exists, the cells contract, fully-fused cells are damaged, and when fluorescein acts on damaged cell membranes, the damage degree of the cell membranes is indirectly reflected according to the amount of leaked fluorescein, so that irritation of the test material is represented. The test material used by the method is stable in population, reliable in source and high in fusing speed, a cell growth culturing membrane is easy to form, and a guarantee is provided for stability of a test; and when the mouse kidney cell system is used for evaluation of irritation damage of the surfactants, a result is in good correlation with a result of a rabbit eye animal test.

Description

technical field [0001] The invention relates to a method for evaluating eye irritation by utilizing mouse kidney cells, which is suitable for evaluating the irritation of cosmetic raw materials, especially for the eye irritation evaluation of surfactants. Background technique [0002] Surfactant is an important fine chemical product, which is widely used at home and abroad. It is known as "industrial monosodium glutamate", which can significantly reduce the surface tension of the solution. Surfactants are widely used in many industrial fields such as cosmetic preparation, food processing, pesticide and pharmaceutical processing, textile printing and dyeing, and detergent production. When surfactants are used in the production of cosmetics or detergents, the probability of contact with consumers' eyes is greatly increased, and they are often irritating or even corrosive to consumers' eyes. [0003] Rabbit eye irritation test (Draize test) is a classic method to detect the ey...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
CPCC12N2503/00G01N33/5044
Inventor 程建华郑华生张鹏谢培镇谢明容陈杰烽黄桂州何衍健
Owner SOUTH CHINA INST OF COLLABORATIVE INNOVATION
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