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Carboxymethyl-beta cyclodextrin-grafted chitosan ionic crosslinking nanoparticles and preparation method and application thereof

An ionic cross-linking, carboxymethyl technology, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of inconvenient injection, inability to achieve therapeutic effect, compliance of susceptible patients, etc., and achieve smooth surface and uniform particle size. , the effect of promoting absorption

Inactive Publication Date: 2017-06-13
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, injection of protein drugs is the most common route of administration, but the inconvenience of injection and the risk of susceptibility to infection lead to poor patient compliance
Oral administration is considered to be the most convenient and comfortable route of administration of protein drugs, but protein drugs are easily denatured by gastric juice and degraded by various proteases, thus failing to achieve a good therapeutic effect

Method used

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  • Carboxymethyl-beta cyclodextrin-grafted chitosan ionic crosslinking nanoparticles and preparation method and application thereof
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  • Carboxymethyl-beta cyclodextrin-grafted chitosan ionic crosslinking nanoparticles and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0030] Preparation of ionically cross-linked nanoparticles grafted with carboxymethyl-β-cyclodextrin and chitosan

[0031] The experimental principle is as follows,

[0032]

[0033] The specific preparation method is as follows:

[0034] a) Activate the carboxyl group of carboxymethyl-β-cyclodextrin: mix 1g carboxymethyl-β-cyclodextrin, 0.5g ethyl[3-(dimethylamino)propyl]carbodiimide hydrochloride and 0.9 g of N-hydroxysuccinimide was dissolved in 20 ml of deionized water, stirred at room temperature for 1 hour to activate the carboxyl group of carboxymethyl-β-cyclodextrin.

[0035] b) Preparation of carboxymethyl-β-cyclodextrin grafted chitosan: prepare a chitosan solution with a concentration of 1%, mix the chitosan solution with activated carboxymethyl-β-cyclodextrin, and Under the conditions of room temperature and a stirring speed of 180 rpm, react for 24 hours, put the reacted mixture into a dialysis bag, place it in deionized water for dialysis, change the deioniz...

Embodiment 2

[0043] Preparation of Chitosan Grafted with Carboxymethyl-β-Cyclodextrin

[0044] The specific preparation method is as follows:

[0045] a) Activate the carboxyl group of carboxymethyl-β-cyclodextrin: mix 1g carboxymethyl-β-cyclodextrin, 0.75g ethyl[3-(dimethylamino)propyl]carbodiimide hydrochloride and 1.2 g of N-hydroxysuccinimide was dissolved in 20 ml of deionized water, stirred at room temperature for 1 hour to activate the carboxyl group of carboxymethyl-β-cyclodextrin.

[0046] b) Preparation of carboxymethyl-β-cyclodextrin grafted chitosan: prepare a chitosan solution with a concentration of 1%, mix the chitosan solution with activated carboxymethyl-β-cyclodextrin, and Under the conditions of room temperature and a stirring speed of 180 rpm, react for 36 hours, put the reacted mixture into a dialysis bag, place it in deionized water for dialysis, change the deionized water every 4 hours, and continue dialysis for 3 days. Centrifuge the product (4000 rev / min), remove...

Embodiment 3

[0049] Preparation of ionically cross-linked nanoparticles grafted with carboxymethyl-β-cyclodextrin and chitosan

[0050] The specific preparation method is as follows:

[0051] a) Activate the carboxyl group of carboxymethyl-β-cyclodextrin: mix 1g of carboxymethyl-β-cyclodextrin, 1g of ethyl[3-(dimethylamino)propyl]carbodiimide hydrochloride and 1.5 g N-hydroxysuccinimide was dissolved in 20ml deionized water, stirred at room temperature for 2 hours to activate the carboxyl group of carboxymethyl-β-cyclodextrin.

[0052] b) Preparation of carboxymethyl-β-cyclodextrin grafted chitosan: prepare a chitosan solution with a concentration of 2%, mix the chitosan solution with activated carboxymethyl-β-cyclodextrin, and Under the conditions of room temperature and a stirring speed of 200 rpm, react for 48 hours, put the reacted mixture into a dialysis bag, place it in deionized water for dialysis, change the deionized water every 4 hours, and continue dialysis for 3 days. Centrif...

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Abstract

The invention discloses carboxymethyl-beta cyclodextrin-grafted chitosan ionic crosslinking nanoparticles and a preparation method and an application thereof. The structural formula of the nanoparticles is as shown in the specification, wherein n is 1200-3200 in the structural formula, the particle size distribution interval is 100-260nm and the mean particle size is about 190nm. Compared with the prior art, a prepared carboxymethyl-beta cyclodextrin-grafted chitosan ionic crosslinking nanoparticle drug carrier has a stable form structure, the particle size distribution interval of the nanoparticles is 100-260nm and the mean particle size is 190nm; hydrophobic protein drugs can be effectively entrapped; and the protection of the protein drugs in the environments of a gastric acid and the like is facilitated.

Description

technical field [0001] The invention belongs to an ion-crosslinked nanoparticle, a preparation method and its application, in particular to a carboxymethyl-β-cyclodextrin grafted chitosan ion-crosslinked nanoparticle, a preparation method and its application Background technique [0002] With the advent of the era of protein biotechnology, many therapeutic protein drugs, such as insulin, growth hormone, interferon, etc. have been marketed. At present, injection of protein drugs is the most common route of administration, but the inconvenience of injection and the risk of susceptibility to infection lead to poor compliance of patients. Oral administration is considered to be the most convenient and comfortable way to administer protein drugs, but protein drugs are easily denatured by gastric juice and degraded by various proteases, so that good therapeutic effects cannot be achieved. Contents of the invention [0003] The first technical problem to be solved by the present...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K47/61B82Y5/00C08G81/00A61K38/00
CPCB82Y5/00A61K38/00C08G81/00
Inventor 龚仁敏宋明明张越李良萍陈款民王慧
Owner ANHUI NORMAL UNIV
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