Application of cordycepin in preparation of medicine for preventing and/or treating radiation damage

A technology of cordycepin and drug, applied in the field of medicine, can solve the problems such as no relevant literature reports on the effect of cordycepin, unclear effect of cordycepin, etc., to enhance antioxidant capacity and colony formation capacity, promote the recovery of hematopoietic system, and reduce cell counts. The effect of vitality

Active Publication Date: 2020-08-21
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of cordycepin on the prevention and treatment of radiation injury is not clear, and the effect of cordycepin on radiation injury has not been reported in the relevant literature

Method used

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  • Application of cordycepin in preparation of medicine for preventing and/or treating radiation damage
  • Application of cordycepin in preparation of medicine for preventing and/or treating radiation damage
  • Application of cordycepin in preparation of medicine for preventing and/or treating radiation damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Detection of Antioxidant Proteins

[0037] Dissolve cordycepin in low-sugar medium, so that the concentration of cordycepin in low-sugar medium is 200 μM. Human dermal fibroblasts were cultured in low-sugar medium containing 200 μM cordycepin for 0, 2, 4, 6, 8, 12, 24, 48, 72, and 96 h, respectively. After the cells were cultured in low-glucose medium containing cordycepin, the cells were collected and protein lysate was added to lyse the cells. The protein concentration was detected by the BCA method. The denatured protein was electrophoresed, transferred to a membrane, and blocked. The primary antibody was blocked overnight and then developed with a secondary antibody. The detected antioxidant proteins include SOD1, SOD2, and GPX-1. The results showed that the levels of antioxidant proteins (SOD1, SOD2, GPX-1) increased in cells pre-cultured with cordycepin ( figure 1 a).

[0038] colony formation assay

[0039] The cordycepin was dissolved in the low-sugar medium, ...

Embodiment 2

[0041] γ-H2AX formation assay

[0042] Dissolve cordycepin in low-sugar medium, so that the concentration of cordycepin in low-sugar medium is 200 μM. Human dermal fibroblasts were cultured in low-sugar medium containing 200 μM cordycepin for 72 hours. After 72 hours, the cells were seeded on coverslips, cultured (low-sugar medium without cordycepin) overnight and irradiated with 5Gy gamma rays. Cells were fixed with 4% paraformaldehyde at different times (0.5, 2, 6, 12, 24 h) after irradiation, cultured in 1% triton solution, blocked with goat serum for 30 minutes, incubated overnight at 4°C with γ-H2AX antibody, and goat anti-mouse The fluorescent secondary antibody was incubated at 37°C in the dark for 2 hours, and after counterstaining the nuclei with DAPI, images were collected with a fluorescence microscope to observe the formation of γ-H2AX. The results showed that, compared with the control cells, cultured with cordycepin could significantly reduce the formation of γ-...

Embodiment 3

[0046] Cell Viability Assay

[0047] Human dermal fibroblasts were seeded in 96-well plates (1×10 4 / well) (low-sugar medium without cordycepin) overnight. Then, 0, 100, 200, and 300 μM concentrations of cordycepin were added to the cells at 37°C, 5% CO 2 Conditioned for 72h. with 900 μM H 2 o 2 After culturing for 12 hours, the relative cell viability was detected by CCK-8 assay. The results showed that compared with the control cells, the cells cultured with cordycepin could significantly reduce the H 2 o 2 Cell viability decreased due to culture ( image 3 ).

[0048] Seed human bronchial epithelioid cells (HBE), tumor A549 and H460 cells in 96-well plates

[0049] (1×104 / well) medium culture (low-sugar medium without cordycepin) overnight. Then, cordycepin at concentrations of 0, 50, 100, 200, and 300 μM was added to the cells and cultured at 37° C. and 5% CO 2 for 72 hours. After 72 h, the relative cell viability was detected by CCK-8 assay. The results showed...

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Abstract

The invention discloses application of cordycepin to preparation of medicines for preventing and / or treating radiation injury and preparation of medicines for sensitizing tumor radiotherapy. Pharmacodynamic experiments prove that the cordycepin can enhance cell anti-oxidation capability and colony forming capability, reduce DNA injury caused by irradiation, reducing hydrogen peroxide injury to cell activity, reduce hemopoietic system injury caused by irradiation, promote recovery of the hemopoietic system, promote healing of wound combined radiation injury, reduce scar formation, reduce skin ulcer and edema caused by radiation, enhance regeneration repair of hypodermal cells and epidermis cells, promote local abdomen irradiation intestine repairing, reduce intestinal injury, sensitize tumor radiotherapy and protect normal tissue cell functions, and new medicines are provided for preventing and treating radiation injury and sensitizing tumor radiotherapy.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of cordycepin in the preparation of drugs for preventing and / or treating radiation damage and preparing tumor radiosensitization. Background technique [0002] Radiation injury refers to acute, delayed or chronic tissue damage caused by ionizing radiation. High-dose radiation can produce visible physical effects within a few days, and the DNA damage caused by radiation can cause serious damage to various tissues and organs of the irradiated person, and even cause genetic defects in offspring. The clinical evolution and severity of radiation injury depend on the type of radiation source, radiation dose and dose rate, exposure site and range, etc. [0003] Radiation damage is divided into whole body radiation damage and partial radiation damage. Radiation damage is not only manifested in the damage to the skin, but also damages other organs and tissues. Whole-bo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7076A61P39/00A61P35/00
CPCA61K31/7076
Inventor 史春梦王子文
Owner ARMY MEDICAL UNIV
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