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Cosmetic composition comprising extracts of fermented usnea longissima using hypha of cauliflower mushroom as active ingredient

A technology of cosmetic composition and active ingredients, which is applied in the direction of medical preparations, cosmetics, and cosmetic preparations containing active ingredients, which can solve the problems of improving elasticity, staying in the skin, and being difficult to obtain, so as to achieve skin safety and reduce cytotoxicity , Improve skin wrinkles and skin elasticity

Active Publication Date: 2017-06-13
COWAY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, cosmetics have only stayed at the level of preventing skin aging, and it is difficult to obtain a substantial effect of improving elasticity.

Method used

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  • Cosmetic composition comprising extracts of fermented usnea longissima using hypha of cauliflower mushroom as active ingredient
  • Cosmetic composition comprising extracts of fermented usnea longissima using hypha of cauliflower mushroom as active ingredient
  • Cosmetic composition comprising extracts of fermented usnea longissima using hypha of cauliflower mushroom as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] Specifically, the preparation method of the usnea tremella fermented extract of the present invention comprises: the step of sterilizing the usnea; moistening the sterilized above-mentioned usnea in the culture medium; inoculating the tremella to the moistened above-mentioned usnea a step of obtaining a fermented product by fermenting the mycelium; and a step of extracting the fermented product with an extraction solvent.

[0026] Hereinafter, each step will be described in detail.

[0027] First, sterilize the usnea.

[0028] Sterilization is to utilize high-pressure steam sterilizer (autoclave) to sterilize all bacterial strains that exist in Usnea, carry out under the routine condition of industry. As an example, the step of sterilizing usnea is carried out by performing heat treatment at a temperature of 100° C. to 125° C. for 5 minutes to 30 minutes.

[0029] Next, the sterilized usnea was moistened in the culture medium.

[0030] By making usnea moist in the cu...

Embodiment 1 to Embodiment 4

[0050] Example 1 to Example 4: Preparation of fermented Usnea extract utilizing white fungus mycelia

[0051] Wet after adding 8 wt% of sterilized usnea to sabouraud dextrose broth. After that, relative to the moist Usnea, inoculate 30wt% of the cultured white fungus hyphae, and culture them statically for 5 days, 10 days, 20 days, and 30 days respectively at a temperature of 30°C. 100 g of the obtained fermented product was put into 70% ethanol with a weight ratio of 20 times and extracted at a temperature of 25° C. for 4 hours, then filtered to remove insoluble substances such as bacteria cells, and the solvent was removed by a rotary vacuum concentrator. The yield of the obtained Usnea fermented extract is shown in Table 1 below.

[0052] Table 1

[0053] distinguish Example 1 Example 2 Example 3 Example 4 Fermentation time (day) 5 10 20 30 Yield (%) 3.22 3.14 1.11 3.42

experiment example 1

[0056] Experimental Example 1: Comparing the collagen synthesis function of Usnea fermented extract according to fermentation time

[0057] Form 1 x 10 per well 6 Cells, fibroblasts as normal human epithelial cells were seeded in 48-well microplates and cultured in DMEM medium for 24 hours. The positive control group and the extracts of Examples 1 to 4 and Comparative Example were added at 100 μg / ml, and cultured for 48 hours. After culturing, the amount of IC-type procollagen titanium (PIP) was measured on the supernatant of each well using a collagen kit (Takara Bioengineering, Takara, Japan), and the amount of collagen synthesized therefrom was measured. figure 1 And Table 2 shows the results.

[0058] Table 2

[0059] distinguish Production of PIP-1 (%) control group 100 positive control group (EGF) 116.3 Example 1 81.7 Example 2 146.6 Example 3 125.7 Example 4 138.7 Comparative example 1 88.1

[0060] refer to ...

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Abstract

The present invention relates to a cosmetic composite having effects of wrinkle improvement and skin elasticity improvement because containing a fermentation extract of usnea longissima sparassis crispa as an active ingredient, wherein the fermentation extract is acquired by extracting a fermented material acquired by fermenting usnea longissima with mycelium of sparassis crispa. According to the present invention, the fermentation using mycelium of sparassis crispa enables cytotoxicity included in the usnea longissima extract to be remarkably reduced, leading to safety in skin, and also enables an excellent combination performance of collagen and elastin that the usnea longissimi could not have. Thus, the present invention is useful in a cosmetic composite for skin wrinkle improvement and skin elasticity improvement.

Description

technical field [0001] The present invention relates to a cosmetic composition in which the Usnea fermented extract of Tremella mycelium is used as an effective ingredients, so it can have the effect of improving wrinkles and improving skin elasticity. Background technique [0002] Skin aging is due to the oxidative damage of organisms by free radicals. In living organisms, the attack of oxidants or free radicals and the defense of antioxidants form a close coordination. However, since the defense of antioxidants is not perfect, biological molecules such as lipids, proteins, nucleic acids, and enzymes, which are the constituent molecules of organisms, are continuously subjected to oxidative damage by oxidants or free radicals, and their oxidation reaction products will be accumulate with age. [0003] In particular, since the skin is always in contact with oxygen, it is subjected to the oxidative burden of exposure to ultraviolet rays, etc., causing damage to the antioxid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/9728A61Q19/08A61Q19/00
CPCA61K8/97A61K8/99A61K2800/85A61Q19/00A61Q19/08
Inventor 田享和洪宇辰
Owner COWAY CO LTD
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