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Core-shell structure nano matrix as well as preparation and application thereof

A core-shell structure, nano-matrix technology, applied in the field of mass spectrometry detection, can solve problems such as low background noise, achieve the effects of improved sensitivity, simple operation, and improved analytical ionization effect

Active Publication Date: 2017-06-09
浙江亿纳谱生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the research of new matrix has gradually become a hot spot, there is no matrix that can combine the advantages of simple preparation process, uniform size, good dispersion, low background noise, good enhancement effect, and accurate quantification.

Method used

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  • Core-shell structure nano matrix as well as preparation and application thereof
  • Core-shell structure nano matrix as well as preparation and application thereof
  • Core-shell structure nano matrix as well as preparation and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0039] SiO 2 The preparation method of @Pt core-shell structure nano-matrix comprises the following steps:

[0040] Step 1: Using ethanol as a solvent, add 2 to 5 ml of ammonia water and stir for 5 to 30 minutes, then add 1 to 5 ml of tetraethyl orthosilicate and continue stirring for 6 to 8 hours to prepare silica pellets with uniform size;

[0041] The second step: ultrasonically disperse the silica pellets obtained in the first step with ethanol, add 3-aminopropyltriethoxysilane (APTES) with water and ethanol as a mixed solvent, and stir at 95°C for 1 hour. to form SiO 2 -NH 2 nanoparticles;

[0042] The third step: the SiO formed in the second step 2 -NH 2 Nanoparticles are dispersed with deionized water, chloroauric acid and sodium hydroxide are added to the solution, stirred and reacted at 75°C for 10 minutes, and annealed with slow stirring for 12 hours to obtain silica gold nanoparticles;

[0043] Step 4: Mix chloroplatinic acid and potassium carbonate at a ratio o...

Embodiment 1

[0049] Embodiment 1 detects glucose standard substance

[0050] Using SiO 2 The steps of matrix-assisted laser desorption ionization time-of-flight mass spectrometry detection of small molecule standards by @Pt core-shell structure nano-matrix are as follows:

[0051] (1) Preparation of instruments and reagents: laser desorption ionization mass spectrometer (MALDI), only mass spectrometry signals with a signal-to-noise ratio greater than 10 are used for analysis. Using AB SCIEX TOF / TOF TM 5800 mass spectrometer, Nd:YAG laser, wavelength 355nm. The pulse electric field delay extraction and reflection working mode is adopted, and the positive ion mode is used for detection. Use DataExplorer to observe, process, and analyze data, and only mass spectrum signals with a signal-to-noise ratio greater than 10 are used for analysis;

[0052] (2) Prepare SiO according to the above method 2 @Pt core-shell nanoparticles;

[0053] (3) Ultrasonic cleaning of the MALDI target plate wi...

Embodiment 2

[0057] Embodiment 2 detects arginine standard substance

[0058] (1) Preparation of instruments and reagents: laser desorption ionization mass spectrometer (MALDI), only mass spectrometry signals with a signal-to-noise ratio greater than 10 are used for analysis. Using AB SCIEX TOF / TOF TM 5800 mass spectrometer, Nd:YAG laser, wavelength 355nm. The pulse electric field delay extraction and reflection working mode is adopted, and the positive ion mode is used for detection. Use DataExplorer to observe, process, and analyze data, and only mass spectrum signals with a signal-to-noise ratio greater than 10 are used for analysis;

[0059] (2) Prepare SiO according to the above method 2 @Pt core-shell nanoparticles;

[0060] (3) Ultrasonic cleaning of the MALDI target plate with formic acid, absolute ethanol, and deionized water in sequence for 1.5 hours, and drying;

[0061] (3) SiO 2 @Pt core-shell nanoparticles composited with arginine in equal volume ratio, specifically, S...

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Abstract

The invention discloses a core-shell structure nano matrix. The core-shell structure nano matrix is of a SiO2@Pt core-shell structure and consists of nano spherical granules, wherein the diameter of the nano spherical granules is smaller than 1[mu] m; the nano spherical granules are uniform in granularity; the nano spherical granules have rough surfaces. The invention further discloses a preparation method and application of the core-shell structure nano matrix in mass spectrum analysis, particularly in mass spectrum analysis on biological samples. By adopting the core-shell structure nano matrix, background noise interference of a conventional organic matrix can be effectively eliminated, and the separation ionization effect of a small-molecular substance can be greatly improved. When the core-shell structure nano matrix is adopted for mass spectrum analysis, molecules of given types can be specifically detected, interference of other molecules can be eliminated, and the core-shell structure nano matrix has certain salt resistance and is beneficial to detection on biological fluid systems. As a rapid and efficient detection method, the core-shell structure nano matrix has very small sample consumption, is beneficial to microminiaturization of a biological sample bank, and is worthy of popularization and application.

Description

technical field [0001] The invention relates to the field of mass spectrometry detection, in particular to the preparation of a core-shell structure nano matrix and its application in mass spectrometry. Background technique [0002] Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is a high-efficiency and high-precision biological detection method developed in recent years, and has been successfully applied to the diagnosis of genome, proteome, metabolome and even clinical medicine. The analysis of the mass-to-charge ratio (m / z) of ions enables accurate qualitative and quantitative analysis of samples. MALDI-MS adopts the matrix-assisted laser desorption ionization technique, that is, the laser is irradiated on the co-crystal formed by the sample to be tested and the matrix, so that the sample to be tested is ionized. In the middle, the matrix plays the role of absorbing laser energy and transferring it to the measured sample, so the matrix needs to ...

Claims

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Application Information

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IPC IPC(8): B22F1/02B22F9/24B82Y40/00B82Y30/00G01N27/62
CPCG01N27/62B82Y30/00B82Y40/00B22F9/24B22F1/17
Inventor 钱昆冯诗婧黄琳
Owner 浙江亿纳谱生命科技有限公司
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