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Ferric oxide substrate, preparation and application of ferric oxide substance in cerebrospinal fluid mass spectrometry

An iron oxide and mass spectrometry analysis technology, applied in the field of mass spectrometry detection, can solve the problems of low background noise and good enhancement effect, and achieve the effects of simple operation, good protein resistance and convenient operation

Active Publication Date: 2017-01-11
浙江亿纳谱生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although research on new matrices has gradually become a hot spot, there is still no matrix that can combine the advantages of simple preparation process, uniform size, good dispersion, low background noise, good enhancement effect, and accurate quantification.

Method used

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  • Ferric oxide substrate, preparation and application of ferric oxide substance in cerebrospinal fluid mass spectrometry
  • Ferric oxide substrate, preparation and application of ferric oxide substance in cerebrospinal fluid mass spectrometry
  • Ferric oxide substrate, preparation and application of ferric oxide substance in cerebrospinal fluid mass spectrometry

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preparation example Construction

[0043] The preparation method of iron oxide matrix, comprises the following steps:

[0044] The first step: using ethylene glycol as a solvent to prepare ferric chloride and trisodium citrate solution;

[0045] Step 2: Add sodium acetate to the above solution, and ultrasonicate at room temperature until the solution is homogeneously dispersed;

[0046] The third step: move the mixed solution obtained in the second step to a polytetrafluoroethylene-lined stainless steel hydrothermal reactor, and keep it at 200 ° C for 10 hours to form iron oxide nanoparticles;

[0047] The fourth step: the iron oxide nanoparticles obtained in the third step were repeatedly washed with ethanol and deionized water, and dried at 60° C. to form a powder.

[0048] Instruments used for characterization:

[0049] The size and morphology of the product were characterized by JEOL JEM-2100F transmission electron microscope (TEM), JEOL JEM-2100F high resolution transmission electron microscope (HRTEM) and...

Embodiment 1

[0052] Embodiment 1 detects glucose standard substance

[0053] The steps for matrix-assisted laser desorption ionization time-of-flight mass spectrometry detection of human cerebrospinal fluid using iron oxide nanoparticle matrix are as follows:

[0054] (1) Preparation of instruments and reagents: laser desorption ionization mass spectrometer, only mass spectrometry signals with a signal-to-noise ratio greater than 10 are used for analysis. Using AB SCIEX TOF / TOF TM 5800 mass spectrometer, Nd:YAG laser, wavelength 355nm. The pulse electric field delay extraction and reflection working mode is adopted, and the positive ion mode is used for detection. DataExplorer was used to observe, process, and analyze data, and only mass spectrometer signals with a signal-to-noise ratio greater than 10 were used for analysis.

[0055] (2) Preparation of iron oxide nanoparticles.

[0056] (3) Composite mass spectrometry analysis of iron oxide nanoparticles and mannitol molecules Composi...

Embodiment 2

[0061] Embodiment 2 detects mannitol standard substance

[0062] (1) Preparation of instruments and reagents: laser desorption ionization mass spectrometer, only mass spectrometry signals with a signal-to-noise ratio greater than 10 are used for analysis;

[0063] (2) Preparation of iron oxide nanoparticles;

[0064] (3) Composite mass spectrometry analysis of iron oxide nanoparticles and mannitol molecules.

[0065] The specific steps are similar to those in Embodiment 1, and will not be repeated here.

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Abstract

The invention discloses a ferric oxide substrate, preparation and application of the ferric oxide substance in cerebrospinal fluid mass spectrometry. The ferric oxide substrate is a nano spherical particle, the size of the ferric oxide substrate is less than 1mu m, the particle size of the particle is uniform, and the nano spherical particle has a rough surface. The ferric oxide substrate can effectively eliminate background noise interference of a conventional organic substrate, so that the parsing ionization effect of small-molecule substances is greatly enhanced, and extremely high salt tolerance and protein tolerance are achieved. On the basis of the ferric oxide substrate, the invention provides a novel method for accurately quantifying molecules in a cerebrospinal fluid system, therefore, precise identification of the small-molecule substances is realized; and furthermore, the sample amount of cerebrospinal fluid can be reduced to a nano liter level, no organic substrate is added in the process, and no sample pretreatment is needed, so that efficient and quick detection of a low-molecular-weight compound is realized.

Description

technical field [0001] The invention relates to the field of mass spectrometry detection, in particular to an iron oxide matrix and its application in cerebrospinal fluid mass spectrometry analysis. Background technique [0002] Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is a new type of soft ionization biological mass spectrometry developed in recent years. Quantitative. MALDI-MS adopts soft ionization technology, and the introduced matrix and sample can form a co-crystal film. Under laser irradiation, the matrix transmits laser energy to the sample molecules to be tested, which can greatly increase the degree of ionization fragmentation of the sample. It can not only be directly applied to the analysis of mixtures, but also can be used to detect whether the sample contains impurities and the molecular weight of impurities. Its accuracy is as high as 0.1% to 0.01%, which is much higher than that of conventional biochemical and high performanc...

Claims

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Application Information

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IPC IPC(8): G01N27/64G01N1/28
Inventor 钱昆黄琳魏翔吴姣
Owner 浙江亿纳谱生命科技有限公司
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