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A kind of noble metal core-shell structure nanoparticle and its preparation and application

A nanoparticle, core-shell structure technology, applied in the field of mass spectrometry detection, can solve the problems of interference of ionization efficiency and low detection efficiency of the sample to be tested, and achieve the effects of less sample consumption, simple operation and convenient operation.

Active Publication Date: 2019-06-04
浙江亿纳谱生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in the detection of complex biological sample systems, due to the presence of a large number of proteins, salts and other substances, the ionization efficiency of the sample to be tested is seriously disturbed, resulting in low detection efficiency

Method used

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  • A kind of noble metal core-shell structure nanoparticle and its preparation and application
  • A kind of noble metal core-shell structure nanoparticle and its preparation and application
  • A kind of noble metal core-shell structure nanoparticle and its preparation and application

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preparation example Construction

[0041] The preparation method of the nanoparticle of silicon core silver shell, comprises the following steps:

[0042] Step 1. Using silver nitrate as a precursor, add ammonia water drop by drop until the yellow-brown precipitate in the solution disappears completely, and the solution is clear and transparent, and then constant volume to prepare 10 -2 M ~ 1M silver ammonia solution;

[0043] Step 2, adding the silicon dioxide dispersion liquid with a mass fraction of 2% to 3% to the silver ammonia solution obtained in step 1, and ultrasonicating at room temperature until the liquid is uniformly dispersed;

[0044] Step 3, adding polyvinylpyrrolidone to the mixed solution obtained in step 2, and reacting at 30-100° C. for 0.5-10 hours to form core-shell structures with different thicknesses;

[0045] Step 4. Repeatedly washing the nanoparticles with core-shell structure obtained in step 3 with ethanol and deionized water, and drying them into powder at 60-70° C. to obtain sil...

Embodiment 1

[0051] Embodiment 1 detects glucose standard substance

[0052] Using silicon-core silver-shell nanoparticles as a matrix to perform matrix-assisted laser desorption ionization time-of-flight mass spectrometry on glucose small molecule standards, the steps are as follows:

[0053] (1) Preparation of instruments and reagents: laser desorption ionization mass spectrometer, only mass spectrometry signals with a signal-to-noise ratio greater than 10 are used for analysis. Using AB SCIEX TOF / TOF TM 5800 mass spectrometer, Nd:YAG laser, wavelength 355nm. The pulse electric field delay extraction and reflection working mode is adopted, and the positive ion mode is used for detection. DataExplorer was used to observe, process, and analyze data, and only mass spectrometer signals with a signal-to-noise ratio greater than 10 were used for analysis.

[0054] (2) Preparation of silicon-core silver-shell nanoparticles.

[0055] (3) The MALDI target plate was ultrasonically cleaned wit...

Embodiment 2

[0059] Embodiment 2 detects arginine standard substance

[0060] Using silicon-core silver-shell nanoparticles as a matrix to perform matrix-assisted laser desorption ionization time-of-flight mass spectrometry detection steps for arginine small molecule standards are as follows:

[0061] (1) Preparation of instruments and reagents: laser desorption ionization mass spectrometer, only mass spectrometry signals with a signal-to-noise ratio greater than 10 are used for analysis. Using AB SCIEX TOF / TOF TM 5800 mass spectrometer, Nd:YAG laser, wavelength 355nm. The pulse electric field delay extraction and reflection working mode is adopted, and the positive ion mode is used for detection. DataExplorer was used to observe, process, and analyze data, and only mass spectrometer signals with a signal-to-noise ratio greater than 10 were used for analysis.

[0062] (2) Preparation of silicon-core silver-shell nanoparticles.

[0063] (3) The MALDI target plate was ultrasonically cle...

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Abstract

The invention discloses a noble metal core-shell nanoparticle, which has a double-layer structure, uses silicon dioxide nanoparticles with uniform particle size as the core, and is continuously coated with silver nanoparticles to form a uniform shell structure; the nanoparticle is spherical Particles, the particle size is not more than 1μm, and the particle size is uniform. The invention also discloses the preparation method of the nano particle and its application in mass spectrometry detection. The core-shell nanoparticle is used in matrix-assisted laser desorption ionization mass spectrometry, which can effectively eliminate the background noise interference of traditional organic matrices and enhance the desorption ionization effect of small molecular substances. It can efficiently identify small molecule metabolites in the actual biological sample system, and can accurately quantify them. The operation process is simple and the detection efficiency is greatly improved.

Description

technical field [0001] The invention relates to the field of mass spectrometry detection, in particular to a noble metal core-shell nanoparticle and its application in matrix-assisted laser analysis ionization mass spectrometry. Background technique [0002] Since the invention of matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) in the 1980s, as a mass spectrometry technique as an emerging soft ionization method, this technology has successfully solved the difficult problem of non-volatile and thermally unstable biological macromolecules. ionization problem. Due to its high sensitivity, simple and convenient operation, MALDI MS has been widely used in the field of biomacromolecules, especially protein research. -12 mol or even 10 -15 It can accurately measure biomacromolecules with a molecular weight of tens of thousands to hundreds of thousands at the mol level, and mass spectrometric detection of macromolecular protein non-covalent complexes can a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F1/02B22F9/24G01N30/00
CPCG01N30/00B22F9/24B22F1/054B22F1/17
Inventor 钱昆黄琳
Owner 浙江亿纳谱生命科技有限公司
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