Pig semen normal temperature liquid negative pressure preservation device and method
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A storage device and pig semen technology, which is applied in the field of animal reproduction, can solve the problems of short storage time, low sperm motility, and poor quality, and achieve the effects of simple operation, improved sperm and semen quality, and high efficiency
Active Publication Date: 2017-06-09
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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[0003] The purpose of the present invention is to solve the problem of short storage time of pig semen in normal temperature and liquid state in the prior art, low sperm motility and poor quality, and further provide a device and method for storage of pig semen in normal temperature and liquid state under negative pressure
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Embodiment 1
[0020] Embodiment 1 (experimental group)
[0021] The method for preserving pig semen in the present embodiment may further comprise the steps:
[0022] Step 1. The fresh pig semen is removed by a centrifuge to remove the upper seminal plasma. The centrifuge rotates at a speed of 1800 r / min, and the centrifugation time is 5 minutes. It was then diluted with Modena diluent to adjust the concentration to 1×10 per ml 8 ~5×10 8 , then detect the sperm motility therein, and after reaching the standard, take 50 milliliters and place it in the semen preservation tube 6;
[0023] Step 2, put the semen storage tube 6 into the airtight container 1, cover the rubber stopper 2 with a valve, and connect the connecting pipe 4 with the vacuum pump 5 with a vacuum gauge; start the vacuum pump 5, and extract the air in the airtight container 1 , so that the air pressure drops to -0.04MPa, close the switch of the valve 3 and the vacuum pump 5, and separate the connecting pipe 4 from the airt...
Embodiment 2
[0025] Embodiment 2 (control group)
[0026] The method for preserving pig semen in the present embodiment may further comprise the steps:
[0027] Step 1. The fresh pig semen is removed by a centrifuge to remove the upper seminal plasma. The centrifuge rotates at a speed of 1800 r / min, and the centrifugation time is 5 minutes. It was then diluted with Modena diluent to adjust the concentration to 1×10 per ml 8 , then detect the sperm motility therein, and after reaching the standard, take 50 milliliters and place it in the semen preservation tube 6;
[0028] Step 2: Put the semen storage tube 6 into a pig semen incubator at 17°C for storage to obtain the required pig semen.
[0029] Comparative Test
[0030] For the different preservation methods of pig semen in Example 1 and Example 2, test operations were carried out respectively. Under different storage days, the values of sperm motility, deformity rate, pH value, active oxygen and total antioxidant capacity of pig s...
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Abstract
The invention relates to a pig semen normal temperature liquid negative pressure preservation device which comprises a closed container, a rubber plug, a connecting tube, a vacuum pump and a semen preservation tube, wherein the rubber plug is arranged at the upper part of the closed container; one end of the connecting tube penetrates through the rubber plug and extends into the inside of the closed container, and the other end of the connecting tube is connected with the vacuum pump; the semen preservation tube is arranged at the bottom inside the closed container. The invention further relates to a method for preserving pig semen by utilizing the device. The method comprises the following steps: removing upper seminal plasma of the fresh pig semen through a centrifugal machine and then diluting the upper seminal plasma with a diluent. The semen preservation tube is put in the closed container, and the closed container is covered with the rubber plug with a valve, and the connecting tube is connected with the vacuum pump with a vacuum meter; the vacuum pump is started to pump air out of the closed container. The pig semen normal temperature liquid negative pressure preservation device provided by the invention has the benefit that as the pig semen is subjected to normal temperature liquid preservation by using the device, the preservation time can be prolonged to 9-13 days, so that the sperm and semen quality are significantly improved.
Description
technical field [0001] The invention relates to the technical field of animal reproduction, in particular to a device and method for storing pig semen in a normal-temperature liquid state under negative pressure. Background technique [0002] In recent years, with the continuous development of the pig industry, the artificial insemination technology of pigs has also been developed rapidly. However, since the semen required for artificial insemination is still mainly stored in a liquid state at room temperature, the quality and timeliness of liquid preservation of pig semen has become a major issue. Key issues restricting pig artificial insemination technology. At present, the semen stored in liquid state at room temperature has problems such as short storage time (generally 3-7 days), low sperm motility after storage, and high deformity rate. Some researchers tried to improve the composition formula of pig semen diluent in order to prolong the storage time of pig semen and ...
Claims
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