Mycobacterium tuberculosis specificity CTL epitope peptides and application thereof

Abstract

The invention relates to mycobacterium tuberculosis specificity CTL epitope peptides and application thereof and belongs to the field of molecular biology and immunology. Through prediction of biological informatics software, selection of HLA-A2 limitation CTL epitope, modification of partial epitope peptides and synthesis and activity comparison and screening of epitope peptides, the obtained four 9-peptides of Rv2629-p190-2L, Rv2629-p190-1Y2L, Rv2629-p274 and / or Rv2629-p315 of the HLA-A2 limitation CTL epitope of mycobacterium tuberculosis protein Rv2629 have higher affinity and higher stability; the IFN-gamma levels for inducing PBMCs generation of a tuberculosis patient are obviously higher than that of a normal control group and a tuberculosis dormant infection group; the CTL ability for stimulating the PBMCs generation of the tuberculosis patient to secrete IFN-gamma is obviously higher than that of the normal control group and the tuberculosis dormant infection group; all the four 9-peptides can induce to generate CTL with killing activity to target cells, and the killing activity can be correspondingly improved along with increasing of effect and target ratio; CTL generated by stimulating modified peptides of Rv2629-p190-2L and Rv2629-p190-1Y2L can identify mother peptide Rv2629-P190. The mycobacterium tuberculosis specificity CTL epitope peptides can serve as a tuberculosis cell-mediated immunity inducer and can be a novel candidate epitope of antituberculous epitope vaccine or an immunity diagnosis reagent.

Description

technical field [0001] The present invention relates to an epitope polypeptide and its application, in particular to a mycobacterium tuberculosis protein (Rv2629) specific cytotoxic T lymphocyte (Cytotoxic T lymphocyte, CTL) epitope polypeptide and its application. These epitope polypeptides can The method can cause specific cellular immune response of tuberculosis patients, has application value in the research and development of tuberculosis immunodiagnostic reagents and epitope vaccines, and belongs to the fields of molecular biology and immunology. Background technique [0002] Tuberculosis is an important public health problem and one of the major infectious diseases threatening human health worldwide, with high morbidity and high mortality. The prevalence of AIDS, the flow of patients with bacterium-expelling tuberculosis, and the poverty of some people have led to an upward trend in the incidence of tuberculosis. In particular, problems such as drug resistance of Myco...

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Problems solved by technology

[0006] Published on June 27, 2012, the patent application number is CN201110362902.6, and the Chinese invention patent titled "Epitope Polypeptides Usable for Detecting Mycobacterium Tuberculosis Infection and Its Application" discloses several Mycobacterium tuberculosis-specific Sexual antigenic epitope polypeptides, especially the antigenic epitope polypeptides of antigen Rv3615c, are 8-11 continuous polypeptide fragments. Although the antigenic polypeptides can generate T cell responses in tuberculosis patients, these polypeptides have neither MHC typing nor For CTL epitope identification, the characteristics of using it as a tuberculosis vaccine are not clear, its affinity and stability need to be identified, and the in vitro immune activity needs to be improved

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