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Method for extracting quercetin from guava leaves

A technology of guava leaves and guava leaf alcohol, applied in the direction of organic chemistry and the like, can solve the problems of lack of systematic research, waste of resources and the like for extracting quercetin, and achieve the effects of high yield, resource saving and high purity

Inactive Publication Date: 2017-05-31
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Quercetin, which is insoluble in water, has good expectorant and cough-relieving effects, and has a certain antiasthmatic effect, exists in the guava leaf residue after extracting the guava leaf extract and is discarded as waste, causing huge waste of resources
There is currently a lack of systematic research on the extraction of quercetin from guava leaves

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The invention provides a method for extracting quercetin from guava leaves, using dried guava leaves as raw materials, comprising the following steps:

[0023] 1) Preparation of guava leaf alcohol extract: add 70v% ethanol with 7 times the mass of guava leaves to the container filled with guava leaves, reflux extraction for 3 times, each time for 1 hour, filter through a 100-mesh sieve, and combine the medicinal solutions , to obtain the guava leaf alcohol extract, measure the total flavone content in the extract by UV method, measure the absorbance of the sample solution at 510nm, and the concentration of the total flavone in the sample solution is 96mg / m ;

[0024] 2) Preparation of guava leaf extract powder: Concentrate the guava leaf alcohol extract by a rotary evaporator at 60°C to an extract with a relative density of 1.1, add water 10 times the mass of the extract, let stand for 12 hours, filter, and Under the condition of 175°C, the inlet air temperature is spra...

Embodiment 2

[0029] The invention provides a method for extracting quercetin from guava leaves, using dried guava leaves as raw materials, comprising the following steps:

[0030] 1) Preparation of guava leaf alcohol extract: add 70v% ethanol with 9 times the mass of guava leaves to the container filled with guava leaves, reflux extraction for 3 times, each time for 1 hour, filter through a 100-mesh sieve, and combine the medicinal solutions , to obtain the guava leaf alcohol extract, measure the total flavone content in the extract by UV method, measure the absorbance of the sample solution at 510nm, and the concentration of the total flavone in the sample solution is 110mg / m ;

[0031] 2) Preparation of guava leaf extract powder: Concentrate the guava leaf alcohol extract by a rotary evaporator at 60°C to an extract with a relative density of 1.2, add water 10 times the mass of the extract, let stand for 12 hours, filter, and Spray-drying is carried out under the condition of 180 DEG C a...

Embodiment 3

[0036] The invention provides a method for extracting quercetin from guava leaves, using dried guava leaves as raw materials, comprising the following steps:

[0037] 1) Preparation of guava leaf alcohol extract: add 70v% ethanol with 10 times the mass of guava leaves to the container filled with guava leaves, reflux extraction for 3 times, each time for 1 hour, filter through a 100-mesh sieve, and combine the medicinal solutions , to obtain the guava leaf alcohol extract, measure the total flavone content in the extract by UV method, measure the absorbance of the sample solution at 510nm, and the concentration of the total flavone in the sample solution is 123mg / m ;

[0038] 2) Preparation of guava leaf extract powder: Concentrate the guava leaf alcohol extract by a rotary evaporator at 60°C to an extract with a relative density of 1.25, add water 10 times the mass of the extract, let stand for 12 hours, filter, and Spray-drying is carried out under the condition of 180 DEG C...

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PUM

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Abstract

The invention provides a method for extracting quercetin from guava leaves. The method comprises the following steps: taking dried guava leaves as a raw material, preparing alcohol extract, preparing extract powder, carrying out recrystallization with boiling water, carrying out recrystallization with ethyl alcohol, and carrying out sephadex column chromatographic separation, so that quercetin is obtained, wherein a TLC method is adopted for identifying quercetin, and an HPLC method is adopted for determining quercetin content. The method provided by the invention has the advantages that waste of quercetin in the guava leaves is effectively reduced, and resource is saved; meanwhile, the raw material is available, environmental pollution is hardly produced, technology is simple, practicable and quick, the obtained quercetin is relatively low in cost, high in purity and high in yield, and the method provided by the invention is applicable to industrial scale production.

Description

technical field [0001] The invention relates to the technical field of medicine manufacture, in particular to a method for extracting quercetin from guava leaves. Background technique [0002] Guava belongs to the family Myrtaceae. Guava leaves contain volatile oil and tannin, which are used for medicinal purposes. It has the effects of stopping dysentery, stopping bleeding, and invigorating the stomach. Because of its remarkable effect of lowering blood sugar, it is often used to treat diabetes abroad. Chinese and foreign folk medicines mostly use guava leaves to treat diseases such as diabetes and diarrhea. Traditional Chinese medicine believes that guava leaves are flat in nature, sweet and astringent in taste, and have the functions of promoting body fluid to quench thirst, eliminating annoyance, astringent and diarrhea, anti-inflammatory, hemostasis, and itching. In 1980, Guangxi Medical College washed and dried the bright green leaves of guava, boiled the dried leaves...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40
Inventor 刘富来陈树华陈俊淼
Owner FOSHAN UNIVERSITY
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