Method for improving gel coagulation property of milk protein concentrates and application of method
A milk protein concentrate and gelling technology, applied in the field of dairy product processing, can solve problems such as affecting the quality of yogurt, and achieve the effect of improving gel strength and water holding capacity
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Embodiment 1
[0030] Embodiment 1: Preparation of modified milk protein concentrate
[0031] (1) Milk protein concentrate (MPC) with a protein content of 70% was dissolved in purified water with a protein content of 3.5% (w / v), stirred slowly with a glass rod for 2 minutes to submerge the powder in the water, and stirred in a constant temperature water bath at 55°C for 2 hours. To obtain the MPC dispersion, in order to prevent the growth of microorganisms, add 0.02g / 100mL sodium azide to the dispersion; then take it out from the water bath, stir it to room temperature (25°C), and place the dispersion at 4°C to ensure that the protein is fully hydrated Store in refrigerator overnight;
[0032] (2) Step (1) Add microbial transglutaminase (MTG) to the dispersion liquid and carry out cross-linking reaction in a constant temperature water bath. The amount of MTG added is 2.0U / g protein, the reaction pH value is 7.0, and the reaction temperature is 33 ℃, the reaction time is 1h, and the pH value...
Embodiment 2
[0043] The amount of MTG added was 2.5U / g protein, the crosslinking reaction temperature was 35°C, and the rest of the steps were the same as in Example 1. The acid gel strength of the modified MPC70 was measured to be 272g, and the water holding capacity WHC was 54%.
Embodiment 3
[0045] The amount of MTG added was 2.5U / g protein, the crosslinking reaction temperature was 35°C, and the pH was 7.25. The rest of the steps were the same as in Example 1. The acid gel strength of the modified MPC70 was measured to be 310g, and the water holding capacity WHC was 64.5%.
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