Artificial maturation method of cephalopods

A cephalopod and artificial technology, applied in the direction of pharmaceutical formula, medical preparations containing active ingredients, animal husbandry, etc., can solve the problems of complex production process and limited release period, and achieve simple operation steps, uniform release speed, and release long time effect

Active Publication Date: 2017-05-31
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This implant also has a second disadvantage: its production process is relatively complicated
This is a consequence of the inner layer not disintegrating, and it limits the release period

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for artificially promoting maturation of cephalopods, the specific steps are: soaking cephalopods in seawater containing 2.5-3.8% polyurethane for anesthesia, preventing inkjet when implanting GnRH slow-release embedding blocks, soaking time is 60 ~100s. A hole was drilled at the orbital sinus of the cephalopod, and the GnRH slow-release embedding block was pushed in with forceps and allowed to float freely in the sinus. Wherein, the preparation steps of the GnRH sustained-release embedding block are:

[0016] 1. Preparation of PLGA powder: Add LA and LG in a molar ratio of 70:30~90:10 into the reaction kettle, vacuumize, and distill under reduced pressure at 70~80°C for 0.8~1.2h to remove 10~20% of the water in the raw materials , add the catalyst stannous chloride, p-toluenesulfonic acid and arachidonic acid, the addition amount is 0.2~0.5%, 0.4~0.8%, 0.001~0.003% of the raw material quality, heating, the temperature is controlled at 120~130 ℃, react in low...

Embodiment 2

[0021] A method for artificially promoting maturation of cephalopods. Taking sea octopus as an example, the sea octopus is anesthetized, and optic glands or GnRH slow-release embedding blocks are implanted in the orbital sinuses under the eyes to achieve the effect of promoting maturation and The purpose of inducing labor. Since the sea octopus is a calcareous endoskeleton, it should not be damaged after implantation; at the same time, because it is loose and porous, it is also easy for hormones to be released into the body to play a role.

[0022] The specific steps are as follows: immerse the squid octopus in seawater containing 3% polyurethane for anesthesia, and prevent inkjet when the GnRH slow-release embedding block is implanted, and the soaking time is 70s. A hole was drilled at the orbital sinus of the sea slug, and the GnRH slow-release embedding block was pushed in with tweezers and allowed to float freely in the sinus. Wherein, the preparation steps of the GnRH su...

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PUM

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Abstract

The invention provides an artificial maturation method of cephalopods, comprising: soaking a cephalopod in sea water containing 2.5-3.8% of polyurethane for anesthesia, drilling at eye socket sinuses of the cephalopod, pushing in GnRH slow-release embedded blocks with tweezers, and allowing the blocks to float freely in the sinuses. The artificial maturation method of cephalopods has the advantages that the GnRH slow-release embedded blocks are implanted in the eye socket sinuses under eyes so that cephalopods are maturated and induced for spawning, and such method allows cephalopods to be maturated quickly with good safety and zero harm; the prepared GnRH slow-release embedded blocks take a long time to release at uniform speed, are biologically degradable, can release completely and may cause zero environmental pollution or energy waste; the operation steps are simple, the results are significant, and artificial breeding of marine cephalopods is never dependent on their natural breeding and egg spawning.

Description

technical field [0001] The invention relates to the field of artificial ripening, in particular to a method for artificial ripening of cephalopods. Background technique [0002] In recent years, the breeding and breeding of marine cephalopods has started in China, and the development momentum is good. However, the artificial breeding of marine cephalopods still relies on their natural reproduction and spawning, and the methods of artificial induction and maturation have not yet been formed. It is not conducive to the large-scale breeding of cephalopods and the development of aquaculture industry. Prior art such as Wells M J, Wells J. Optic glandimplants and their effects on the gonads of Octopus. [J]. Journal of Experimental Biology, 1975, 62 (3): 579-88. Show: the vision of two-toed cephalopods Glands can secrete hormones to control the state of the gonads and their pipelines, and can force animals to mature prematurely by cutting off the inhibitory nerves to the optic gla...

Claims

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Application Information

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IPC IPC(8): A01K61/50A61K38/09A61P15/08
CPCA01K61/00A61K38/09Y02A40/81
Inventor 刘立芹王茂廷龚理王天明黄伟刘慧慧杨静文吴常文
Owner ZHEJIANG OCEAN UNIV
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