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Serum amyloid A testing kit and manufacturing method

A technology of serum amyloid and kit, which is applied in the field of membrane chromatography technology, protein cross-linking technology, serum amyloid A assay kit, and marker immunoassay technology. It can solve the problems of high intensity and strong interference and achieve rapid response. , high sensitivity and strong specificity

Inactive Publication Date: 2017-05-24
WEIHAI NEOPROBIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the usual fluorescence measurement, because the test sample contains a variety of fluorescent components, the background fluorescence (scattered light caused by colloidal particles and solvent molecules in the sample and non-specific fluorescence emitted by proteins and other compounds in the serum) has a large intensity and interference. Strong, become the bottleneck of large-scale promotion of fluorescence analysis

Method used

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  • Serum amyloid A testing kit and manufacturing method
  • Serum amyloid A testing kit and manufacturing method
  • Serum amyloid A testing kit and manufacturing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Each component of the test paper card in the serum amyloid A assay kit can be prepared by the following measures:

[0043] 1. Preparation of sample pad 2:

[0044] Soak the glass fiber membrane in the treatment solution containing 1.0% Triton X-100, 2.5% BSA, 0.15M Tris buffer, pH7.5, soak at 4°C for 4 hours, then place it in an oven and dry it at 37°C 2 hours.

[0045] 2. Preparation of binding pad 3 for absorbing fluorescent microsphere-labeled antibody:

[0046] Soak the glass fiber membrane in 150mM Tris-HCL treatment solution (containing 1.0% Triton X-100, 2.5% BSA, pH7.4), soak for 2 hours at 4°C, then take it out of the oven at 37°C and dry it for 4 hours, and set aside. The glass fiber membrane was placed on the Bio-DotXYZ3050 three-dimensional spraying platform, and the rare earth Eu 3+ The anti-serum amyloid A monoclonal antibody-conjugated complex labeled with fluorescent microspheres is sprayed onto a glass fiber membrane, and dried at 37°C for 1 hour.

...

Embodiment 2

[0055] Embodiment 2: accuracy test

[0056] Select the above test paper card and fluorescence immunochromatography analyzer (model: NEO-007),

[0057] Setting of the parameters of the fluorescence immunoassay analyzer: After setting the process parameters of the test paper card on the fluorescence immunoassay The serum amyloid A calibrator is measured with a test paper card to obtain the fluorescence intensity value of each calibrator, and the result is input into the parameters of the analyzer to complete the setting of the parameters of the analyzer.

[0058] Main testing materials: clinical samples obtained from relevant hospitals, a total of 300 latex-enhanced immunoturbidimetric value samples, including 100 serum samples, 100 plasma samples, 100 whole blood samples, and the serum amyloid A content distribution interval is 5.00 Between -200mg / L.

[0059] Detection method:

[0060] Step 1: Equilibrate the detection reagent and sample to room temperature, take out the tes...

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Abstract

The invention relates to the technical field of fluorescence immunoassay in medical immunology, and in particular relates to a serumamyloid A (SAA) testing kit (fluorescence immunochromatographic method) and a manufacturing method. The kit is provided with a test paper card. The kit is characterized in that a PVC (Polyvinyl Chloride) plate, a sample pad, a conjugate pad, a nitrocellulose membrane and a water absorbing pad are sequentially arranged on the test paper card from bottom to top; a rare-earth Eu<3+> fluorescent microsphere marked serum amyloid A monoclonal antibody is adsorbed on the conjugate pad; the diameter of a rare-earth fluorescent microsphere is 200nm; the rare-earth fluorescent microsphere contains a rare-earth lanthanide element Eu<3+>; the rare-earth fluorescent microsphere is stable in a ground state and emits fluorescence of which the wavelength is 615nm under the stimulation action of laser of 337nm; and the monoclonal antibody is a monoclonal antibody which is purified and mixed, and comes from a monoclonal antibody cell strain for 2-6 different serum amyloid A epitopes. The kit has the advantages of being simple and convenient to operate, rapid in reaction, high in sensitivity, good in specificity and the like.

Description

[0001] Technical field: [0002] The invention relates to the technical field of fluorescence immunochromatography in medical immunology, including protein cross-linking technology, membrane chromatography technology, marker immunoassay technology and the like. Specifically, it is a serum amyloid A assay kit capable of quickly and accurately performing quantitative analysis on serum amyloid A in samples such as serum, plasma, and whole blood, and a production method thereof. [0003] Background technique: [0004] Serum amyloid A (SAA) is the serum precursor of amyloid A protein, the main component of amyloidosis. It is an acute phase protein secreted into serum after being produced by liver cells. Although it can be seen everywhere, it is in Under normal circumstances, the expression in the human body is still very low. However, once inflammation, infection or tissue damage occurs in the body, the level of SAA can rapidly increase by about 1,000 times within 5-6 hours. Studies...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/533G01N33/543
CPCG01N33/68G01N33/533G01N33/54346
Inventor 李红江于鸿翔王文亮刘衍亮陈萍萍宋璐琳
Owner WEIHAI NEOPROBIO
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