An electrochromatographic device

Abstract

The invention discloses an electrochromatography device, which comprises an infusion system, a shunting system, a quantitative sampling system, a current circuit system, a chromatographic separation system, a temperature control system, a detector, a data processing system and a liquid waste pool, wherein the infusion system is introduced on the basis of reserving the advantages of a traditional separate analysis device and the work which cannot be done by a traditional electrophoresis instrument can be done; the quantitative sampling system is introduced and the problems of cross contamination, poor quantitative reproducibility and the like of a traditional electrophoresis apparatus are solved; the current circuit system is introduced and the multi-mode separate mode can be achieved by only one set of device; a novel chromatographic separation system (open tube-type chromatographic column) is introduced, the whole device has the selectivity of a high-performance liquid chromatographic instrument and is a potential separate analysis device while the problems that a traditional packed column is difficult to prepare, easy to block, short in service life and high in cost are solved; and the electrochromatography device is stable, reliable and simple in operation, and is expected to be popularized and applied to multiple frontier domains of biological medicines, environment analysis, bioscience and the like.

Description

technical field [0001] The invention belongs to the field of electrophoresis and chromatographic separation analysis, and in particular relates to an electrochromatographic device. Background technique [0002] Capillary electrochromatography (CEC) is an emerging high-efficiency separation and analysis technology in recent years. It uses electroosmotic flow or electroosmotic flow combined with pressure flow to drive the mobile phase, and has the dual separation mechanism of capillary electrophoresis and high performance liquid chromatography. Both charged and neutral substances can be separated. However, the traditional electrochromatographic device is composed of a high-voltage power supply, a capillary chromatographic column (packed column) with packing and detection equipment. On the one hand, the existence of "plugs" at both ends of the packed column makes the column easy to be burned out during electrochromatographic operation. , bubbles appear, leading to the interrup...

AI Technical Summary

Problems solved by technology

However, the traditional electrochromatographic device is composed of a high-voltage power supply, a capillary chromatographic column (packed column) with packing and detection equipment. On the one hand, the existence of "plugs" at both ends of the packed column makes the column easy to be burned out during electrochromatographic operation. , bubbles appear, leading to the interruption of the separation work; on the other hand, the particle size of the filler is getting smaller and smaller, and the filling process is becoming more and more difficult, which restricts the development of packed column electrochromatography. Sometimes, it is easier to accumulate and block the column, thereby greatly reducing the service life of the analyzer; finally, the traditional electrochromatographic device does not solve the problem of accurate sample injection

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