Application of yeast culture in preparation of feed capable of improving reproductive performance and relieving constipation of sows
A technology of yeast culture and reproductive performance, applied in the field of animal breeding feed, can solve problems such as less research
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Embodiment 1
[0028] Embodiment 1, the preparation of yeast culture
[0029] 1. Activation of slant strains: inoculate the Saccharomyces cerevisiae with the preservation number of CGMCC No.2.399 frozen and preserved on the slant medium containing malt extract powder 130g / L, chloramphenicol 0.1g / L, and agar 2%. Keep the temperature at 30°C and culture for 48 hours to obtain activated yeast strains.
[0030] 2. Preparation of seed liquid: Inoculate the activated yeast strain with 1 loop into liquid medium containing malt extract powder 130g / L, chloramphenicol 0.1g / L; pH value is 6.5, and shake at a constant temperature of 30°C Bed culture, rotation speed 160r / min; culture 24h.
[0031] 3. Liquid aerobic fermentation: Inoculate the prepared seed liquid with 20% inoculation amount into a new liquid culture medium containing malt extract powder 130g / L, chloramphenicol 0.1g / L; pH value 5.6 Expanded culture was carried out in the tank, the ventilation rate was controlled at 2 L / min, the temperat...
Embodiment 2
[0035] Embodiment 2, sow reproductive performance and the impact test of sow constipation
[0036] 1. Feed Preparation
[0037] The basic diet used in this experiment included corn, soybean meal, wheat bran, soybean oil, sodium chloride, 50% choline chloride, stone powder, calcium hydrogen phosphate, 78% L-lysine, 98% DL-methionine, Premix, wherein, the premix provides vitamin A, 10500IU per kilogram of ration; vitamin D 3 , 1500IU; Vitamin E, 15IU; Vitamin K 3 , 1.6mg; Vitamin B 1 , 1.5mg; Vitamin B 2 , 3.0mg; Vitamin B 6 , 1.5mg; Vitamin B 12 , 0.015mg; Niacin, 22.5mg; Pantothenic acid, 15mg; Biotin, 40mg; Folic acid, 2.5mg; Manganese (MnSO 4 ·H 2 O), 40 mg; Iron (FeSO 4 ·H 2 O), 85mg; Zinc (ZnSO 4 ·H 2 O), 75 mg; Copper (CuSO 4 ·5H 2 O), 1.5 mg; iodine (Ca(IO 3 ) 2 ), 0.09mg; Selenium (Na 2 SeO 3 ), 0.03mg of mixture.
[0038] The addition amount of each component of the above-mentioned basal diet is shown in Table 1 below.
[0039] 2. Test process
[0040...
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