Special primer for double PCR of contagious pustular dermatitis virus and mycoplasma ovipneumoniae

Abstract

The invention provides a special primer for double PCR of a contagious pustular dermatitis virus and mycoplasma ovipneumoniae. According to the special primer, two pairs of specific primers capable of amplifying a B2L gene and a P80 gene are designed according to the B2L gene of the contagious pustular dermatitis virus and the P80 gene of the mycoplasma ovipneumoniae. Through optimization of the conditions of the primer concentration, the annealing temperature and the like, a double PCR method for fast detecting the contagious pustular dermatitis virus and the mycoplasma ovipneumoniae is built. A specific segment of 401bp appearing in an electrophoretogram has positive contagious pustular dermatitis virus, and the specific segment of 700bp has positive mycoplasma ovipneumoniae. Detection of the contagious pustular dermatitis virus and the mycoplasma ovipneumoniae in the same reaction system can be achieved, the special primer has the advantage that the pathogens are fast, specifically and accurately diagnosed, and a novel method is provided for differential diagnosis of infection of the two pathogens in production and epidemiological investigation.

Description

Technical field [0001] The invention relates to a special primer for double PCR of sheep infectious pustular virus and Mycoplasma ovis pneumoniae and a double PCR detection method, belonging to the field of preventive veterinary medicine. Background technique [0002] Contagious ecthyma (CE), commonly known as sheep aphthous (Orf), is an acute, contact and addictive disease of goats, sheep and humans caused by Orf virus (ORFV) infection. Epithelial zoonotic disease. The incidence of the disease is 4%-100%, and the mortality rate is 1%-59%. Mycoplasma ovipneumoniae (Mo) is one of the main pathogens that cause Mycoplasma pneumonia in sheep and goats. The clinical symptoms of Mycoplasma pneumonia are high fever, cough, gasping, progressive weight loss, serous and fibrinous inflammation of lung and pleura. The incidence rate is 20%-60%, the case fatality rate is generally 30%-50%, and some are as high as 80%. Mo has a complex structure and difficult cultivation. There is still a l...

AI Technical Summary

Problems solved by technology

Some scholars at home and abroad have used PCR technology to diagnose and epidemiologically study sheep contagious impetigo and goat contagious pleuropneumonia. In China, Qu Yonggang et al., Li Yuan et al., and Chu Yuefeng et al. respectively established PCR detection methods for ovine mycoplasma pneumoniae. and Mycoplasma ovis pneumoniae and mycoplasma mycoplasma goat subspecies double PCR detection method, Yan Xinmin et al established a double PCR method for the rapid differential diagnosis of sheep pox virus and aphthus virus, but there is no simultaneous rapid detection of sheep infectious pustules at home and abroad Report on the method of virus and Mycoplasma pneumoniae

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