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Anti-IL-4R single-chain antibody and snake venom L-amino acid oxidase fusion protein and application thereof

A single-chain antibody, fusion protein technology, applied in the biological field, can solve problems such as short half-life and clinical application limitations

Active Publication Date: 2017-05-10
贵州中医药大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the mouse-derived monoclonal antibody is a heterologous protein antigen to the human body, it can induce the human immune system to produce human anti-mouse antibody (HAMA), and the half-life of the mouse-derived monoclonal antibody in the human body is short (1-2d), so the clinical application is limited
However, there is no report on the construction of a fusion protein of anti-IL-4R scFv and LAAO for targeted therapy of lung cancer.

Method used

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  • Anti-IL-4R single-chain antibody and snake venom L-amino acid oxidase fusion protein and application thereof
  • Anti-IL-4R single-chain antibody and snake venom L-amino acid oxidase fusion protein and application thereof
  • Anti-IL-4R single-chain antibody and snake venom L-amino acid oxidase fusion protein and application thereof

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Embodiment 1

[0112] A fusion protein of anti-IL-4R single-chain antibody and snake venom L-amino acid oxidase, the preparation of the fusion protein specifically includes the following steps:

[0113] (1) Select the scFv gene sequence whose restriction sites are EcoRI and XhoI, and connect the scFv and LAAO genes by overlapping extension PCR technology. Add an XhoI restriction site at the 3' end of the venom LAAO gene sequence, add an overlapping sequence identical to the scFv gene sequence of the anti-IL-4R single-chain antibody, and a linking peptide linking gene at the 5' end of the venom LAAO gene sequence. Add an XhoI restriction site at the 3' end of the "overlapping sequence" gene sequence;

[0114] Wherein, primer F1 and primer F2 are designed to remove the Xho I restriction site of the anti-IL-4R single-chain antibody scFv gene sequence;

[0115] (2) Design primers F1 and F3, remove 15 μL of scFv gene fragment of XhoⅠ restriction site, 15 μL of LAAO gene fragment, 1 μL of primer ...

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Abstract

The invention discloses anti-IL-4R single-chain antibody and snake venom L-amino acid oxidase fusion protein; the fusion protein is prepared by: connecting anti-IL-4R single-chain antibody and Trimeresurus stejnegeri venom LAAO (L-amino acid oxidase) gene into scFv-LAAO fusion gene by means of overlap extension PCR (polymerase chain reaction) technology, constructing recombinant plasmid pET28-a-scFv-LAAO, transferring to BL21(DE3) prokaryotically-expressed bacterium, and inducing to express scFv-LAAO fusion protein. By using molecular biological techniques, an expression vector for anti-IL-4R single-chain antibody gene (scFv) and snake venom L-amino acid oxidase fusion gene is used to carry out in-vitro expression in order to recombine the anti-IL-4R single-chain antibody and snake venom L-amino acid oxidase fusion protein, and the anti-IL-4R single-chain antibody and snake venom L-amino acid oxidase fusion protein is applicable to the treatment of lung cancer, has good therapeutic effect and is substantially free of side effects.

Description

technical field [0001] The invention relates to a fusion protein of anti-IL-4R single-chain antibody and snake venom L-amino acid oxidase and application thereof, belonging to the field of biotechnology. [0002] technical background [0003] Lung cancer is the malignant tumor with the highest morbidity and mortality, which poses a great threat to human life and health. It is the first cause of death from malignant tumors, and its incidence is still on the rise. Surgical resection is the most effective treatment for early lung cancer, but patients have higher systemic and local recurrence rates after surgery. The chemotherapy effects of traditional cytotoxic drugs have reached a plateau, and there is very little room for further development, and the side effects of traditional cytotoxic chemotherapy drugs also limit their application and development. Radiation therapy is no longer suitable for primary tumors that have developed. Radiotherapy combined with chemotherapy can i...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/70A61K38/44A61K47/68A61P35/00
CPCA61K38/00C07K16/2866C07K2317/622C07K2319/33C07K2319/55C12N9/0022C12N15/62C12N15/70C12Y104/03002
Inventor 何光志杨光勇郭海涛刘茜明田维毅梁建东蔡琨王平王文佳韩洁
Owner 贵州中医药大学
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