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Support material with function of promoting differentiation of stem cells as well as preparation method and application of support material

A scaffold material and carbon-based nanomaterial technology, applied in the field of scaffold materials, can solve problems such as weak bone regeneration ability, difficulty in self-repair of defective bone, and inability to effectively induce autologous bone regeneration.

Active Publication Date: 2017-05-10
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bioscaffolds currently under development generally have the problem of biological inertness, which is mainly manifested in the lack of osteoinductive activity and cannot effectively induce autogenous bone regeneration, especially for orbital bone with thin walls, less bone marrow cavity, poor local blood supply, and weak bone regeneration ability. bone tissue, the self-repair of defective bone is more difficult
In order to improve the osteoinductive activity of scaffold materials, a large number of studies have used methods such as stem cell gene regulation (Int.J.Mol.Med., 2010, 26, 517-525) and growth factor induction (PNAS, 2014, 111, 12847-12852 ; Biomacromolecules, 2014, 15, 1019-1030) improve the ability of scaffold materials to repair bone defects, but face new problems such as biological safety, growth factor activity and economic cost

Method used

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  • Support material with function of promoting differentiation of stem cells as well as preparation method and application of support material
  • Support material with function of promoting differentiation of stem cells as well as preparation method and application of support material
  • Support material with function of promoting differentiation of stem cells as well as preparation method and application of support material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 Scaffold material repairs calvarial defect in rats

[0040] (1) Take by weighing 4g carboxymethyl chitosan (CMC) and dissolve in 100mL deionized water to obtain 4% (w / v) CMC aqueous solution; Take by weighing 0.4g graphene oxide (GO) and dissolve in 100mL deionized water, and Sonication (200W, 80%) for 1 hour gave a yellow-brown transparent 0.4% (w / v) GO aqueous solution.

[0041] (2) Take 5mL 4% (w / v) CMC aqueous solution and 5mL 0.4% (w / v) GO aqueous solution and magnetically stir and mix them, and then place them in an ultrasonic generator for ultrasonic dispersion (200W, 80%) for 1h to obtain 2% ( w / v) Well mixed aqueous solution of GO and 0.2% (w / v) CMC.

[0042] (3) Weigh 0.1 g of nHA and add it to the above mixed aqueous solution, and disperse it by ultrasonic (200 W, 80%) for 1 h to obtain a uniformly dispersed nHA / GO / CMC mixed solution.

[0043] (4) Add 1mL of 1mM EDC aqueous solution and 250μL of 1mM NHS aqueous solution to the above 10mL nHA / GO...

Embodiment 2

[0051] Example 2 Scaffold material repairing canine orbital bone defect

[0052] (1) Weigh 4g collagen (Col) and dissolve it in 100mL deionized water to obtain a 4% (w / v) Col solution; weigh 0.4g GO and dissolve it in 100mL deionized water, and ultrasonically (200W, 80%) 1 A tan transparent 0.4% (w / v) GO aqueous solution was obtained after 1 hour.

[0053] (2) Take 5 mL of 4% (w / v) Col aqueous solution and 5 mL of 0.4% (w / v) GO aqueous solution and magnetically stir and mix them, and then place them in an ultrasonic generator for ultrasonic (200W, 80%) dispersion for 1 h to obtain 2% ( w / v) Well mixed aqueous solution of Col and 0.2% (w / v) GO.

[0054] (3) Weigh 0.2g nano silicon dioxide (nSiO 2 ) into the above mixed aqueous solution, and ultrasonically (200W, 80%) dispersed for 1h to obtain uniformly dispersed nSiO 2 / GO / Col mixed solution.

[0055] (4) Take the above 10mL nSiO 2 Add 1mL of 1mM EDC aqueous solution and 250μL of 1mM NHS aqueous solution to the / GO / Col mi...

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Abstract

The invention relates to a support material which comprises a carbon-based nano material, natural macromolecules and a one-dimensional inorganic nano material; the carbon-based nano material is crosslinked with the natural macromolecules through amide bonds; the natural macromolecules are self-crosslinked through the amide bonds; the one-dimensional inorganic nano material absorbs the natural macromolecules. The support material provided by the invention not only can induce the differentiation of the stem cells through the chemical activity of the nano material (such as osteogenic differentiation), but also can regulate and control the differentiation of the stem cells through a micro-nano structure on the surface of the support material (such as osteogenic differentiation), and has the function of promoting the regeneration and the repair of defective tissues (such as bone tissues) in vivo.

Description

technical field [0001] The invention relates to a scaffold material, in particular to a scaffold material cross-linked by several materials, which has the function of promoting stem cell differentiation, as well as a preparation method and application. Background technique [0002] Traumatic fractures, inflammation, tumors, and congenital abnormalities can all cause bone defects. Implanting artificial scaffold materials into bone defects is an internationally recognized method for bone defect repair. The bioscaffolds currently under development generally have the problem of biological inertness, which is mainly manifested in the lack of osteoinductive activity and cannot effectively induce autologous bone regeneration, especially for orbital bone with thin walls, less bone marrow cavity, poor local blood supply, and weak bone regeneration ability. The bone tissue, the self-repair of the defect bone is more difficult. In order to improve the osteoinductive activity of scaffo...

Claims

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Application Information

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IPC IPC(8): A61L27/08A61L27/22A61L27/20A61L27/12A61L27/02C12N5/077C12N5/0775
CPCA61L27/02A61L27/08A61L27/12A61L27/20A61L27/22A61L2430/02C12N5/0663C12N5/0667C12N2533/20C12N2533/54C12N2533/70C08L89/00C08L5/08
Inventor 范先群阮静
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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