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Novel application of leonurine

A technology for leonurine and depression is applied in the application field of leonurine in the preparation of drugs for treating or preventing depression, and achieves the effect of high safety

Active Publication Date: 2017-05-10
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the effect of motherurine on depression has not been reported so far. The present invention confirms the possibility of motherurine as a drug for preventing and treating depression from various aspects through relevant experimental research.

Method used

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  • Novel application of leonurine
  • Novel application of leonurine
  • Novel application of leonurine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Improvement effect of motherwortine on depression-like behavior in CMS mice

[0039] The present invention adopts the CMS depression model recognized by the international academic circle to prepare rodent depression. Male C57BL / 6J mice, weighing 18-22 g, aged 2-3 months, were purchased from the Comparative Medicine Center of Yangzhou University (animal production license number is SCXR (Su) 20120004). After one week of adaptive feeding, the mice in the normal control group ate and drank normally without any stimulation, and the stressed mice received two or three random, mild and unpredictable stimuli every day until depression-like symptoms appeared. The CMS method includes a series of unpredictable chronic mild stresses including: tail pinching, circadian reversal, 45° inclined cage (6h), restraint (12h), wet cage (12h), empty cage (10-14h), water deprivation (15h), pairing (2h), changing bedding, strobe lighting for 12h, etc. The same stimulus cannot be g...

Embodiment 2

[0048] Example 2: Effects of Leonurine on Monoamine Neurotransmitters in the Brain of CMS Mice

[0049] In this part, the CMS depression model described in Example 1 was used. Up to the sixth week, leonurine (30 mg / kg / day or 60 mg / kg / day) was given by intragastric administration for 4 weeks. 1h after the last administration, the mice were deeply anesthetized, the brain tissue was quickly peeled off, the hippocampus and prefrontal cortex were separated, and the homogenate (0.1M HClO 4 , 0.1mM EDTA) homogenate, centrifuge (20,000rpm, 30min), take the supernatant, and Thermo ultimate 3000 high performance liquid chromatography system (HPLC) detects the monoamine neurotransmitter 5-hydroxytryptamine (5-HT), Norepinephrine (NE) and dopamine (DA) levels.

[0050] The results of the determination of monoamine transmitters in the hippocampus (Table 3, attached Figure 4 As shown in A-4C), compared with the normal control group, the contents of 5-HT, NE, and DA in CMS-stressed mice d...

Embodiment 3

[0056] Example 3: Effects of Leonurine on Neurons and Glial Cells in the Brain of CMS Mice

[0057] In this part, the CMS depression model described in Example 1 was adopted, and until the sixth week, leonurine (30 mg / kg / day or 60 mg / kg / day) was given by intragastric administration for 4 weeks. 1 hour after the last administration, the mice were deeply anesthetized, perfused with 4% paraformaldehyde, and dehydrated with gradient sucrose. The brain tissue was frozen and sectioned, and the hippocampal brain slices were selected according to the brain atlas for Nissl staining to observe the morphology and structure of hippocampal neurons; in addition, at 4 % (mass percentage) after paraformaldehyde perfusion, the isolated hippocampus was fixed in 2.5% (mass percentage) glutaraldehyde, the electron microscope specimen was prepared, and the ultrastructure of the hippocampal neurons was observed under the transmission electron microscope; the immunofluorescence method was used to det...

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Abstract

The invention belongs to the field of traditional Chinese medicine pharmacy and relates to application of leonurine in the preparation of drugs for preventing or treating depression. After depression-like symptoms in a mouse are induced through a chronic mild stress model, leonurine is given via gavage; the results indicate that leonurine can significantly improve depression-like behaviors of the CMS mouse, increase the mass transfer contents of 5-HT, NE and DA monoamines, improve hippocampal neuronal injury, increase the number of astroglia cells in sea horse, inhibit cerebral neuritis, and increase the levels of cerebral nerve growth factors BDNF and GDNF. It can be seen from the invention that leonurine resists depression in multiple terms and is suitable for the preparation of drugs for preventing or treating depression.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine preparations and relates to a new medicinal application of motherurine, in particular to the application of motherurine in the preparation of medicines for treating or preventing depression. Background technique [0002] Depression is a complex and long-lasting emotional disorder, also known as depressive disorder. Its main clinical manifestations are marked and persistent depression, loss of interest, emotional depression, and even pessimistic world-weariness, suicide attempts and other behaviors. According to statistics, since 2004, depression has become the third most persistent disease in human beings, and it is estimated that by 2030, depression may become the number one disease affecting human life. [0003] A large number of studies have shown that in addition to many factors in the social environment, depression still has pathological hypotheses such as lack of monoamine neurotr...

Claims

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Application Information

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IPC IPC(8): A61K31/235A61P25/24
CPCA61K31/235
Inventor 胡刚贾苗苗朱依谆丁建花鲁明
Owner NANJING MEDICAL UNIV
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