A Triangular Optimization Method for the Separation of Targets from Complex Compounds by Simulated Moving Bed Chromatography

A technique for simulating moving bed and chromatographic separation, applied in the field of triangle optimization, to achieve the effect of simplifying the optimization process

Active Publication Date: 2019-03-26
UNIV OF SCI & TECH LIAONING
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a triangular optimization method for the separation of complex mixtures with simulated moving bed chromatography for the deficiencies in optimization techniques when complex mixtures are separated by existing SMB chromatography

Method used

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  • A Triangular Optimization Method for the Separation of Targets from Complex Compounds by Simulated Moving Bed Chromatography
  • A Triangular Optimization Method for the Separation of Targets from Complex Compounds by Simulated Moving Bed Chromatography
  • A Triangular Optimization Method for the Separation of Targets from Complex Compounds by Simulated Moving Bed Chromatography

Examples

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Embodiment

[0061] A Triangular Optimization Method for the Separation of Rebaudioside A (RA) from Stevia Rebaudiana Extract by SMB Chromatography.

[0062] The SMB chromatographic system used: working mode: 1-1-2, I band is independent; 4 chromatographic columns, the chromatographic column specification is D×L=10mm×100mm; stationary phase: C18, 40-60 μm; mobile phase: ethanol-water , ratio 1:1; chromatographic separation temperature: room temperature.

[0063] HPLC detection conditions: analytical column: 4.6mm * 250mm (C18, 5 μm, Agilent, U.S.A.), mobile phase: the ratio of chromatographic acetonitrile and sodium dihydrogen phosphate buffer solution of pH=2.6 is 32 / 68 (v / v), flow velocity 1.0ml / min, measurement wavelength 213nm, measurement temperature 30°C.

[0064] The optimization method includes the following steps:

[0065] (1) prepare sample liquid by the proportioning of mobile phase ethanol and water of SMB chromatography, measure the concentration C of RA in sample liquid wit...

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Abstract

The patent discloses a triangular optimization method for separating targets in complex substances by simulated moving bed chromatography, which belongs to the technical field of chromatography. This method first determines the interfering components of the target in the SMB separation system, and assigns the concentration of the interfering components, then establishes a single-column ideal chromatographic model, and obtains the adsorption isotherm of the target and interfering components of the SMB separation unit through the inverse method. It is closer to the dynamic characteristics of the chromatographic process, and thus obtains the apparent adsorption coefficient of fast component A and slow component B, and controls the relative flow rate ratio m of band II and band III of SMB α In the triangular region formed by the apparent adsorption coefficients of fast component A and slow component B, the operating parameters of nonlinear SMB separation of target components and interference components can be optimized.

Description

technical field [0001] The invention relates to the technical field of chromatography, in particular to a triangular optimization method for separating target objects in complex objects by simulated moving bed chromatography. Background technique [0002] Simulated moving bed (SMB) chromatographic technology has the characteristics of continuous, automatic and efficient, and has become a high-end separation technology in the fields of petrochemical, fine chemical and pharmaceutical (especially chiral drug resolution and biological product separation). Generally speaking, the operating variables of SMB include: switching period, sample liquid composition and concentration, mobile phase circulation flow rate, mobile phase inlet flow rate, extraction liquid flow rate, sample liquid flow rate and residual liquid flow rate; SMB inspection performance indicators include: product Purity, recovery, yield, mobile phase consumption and stationary phase usage. In order to maximize the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 王绍艳张志强路来菊
Owner UNIV OF SCI & TECH LIAONING
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