Material supplementing method for reducing viscosity of gentamicin C1a fermentation liquid

Abstract

The invention relates to a material supplementing method for reducing viscosity of a gentamicin C1a fermentation liquid, and belongs to the field of fermentation engineering, in the whole fermentation process only control of supplementing of two materials of carbon and nitrogen is performed in the method, reducing sugar concentration in a fermentation pot is controlled in 1-20g / L, when the reducing sugar concentration is lower than 5g / L, a continuous flow adding method is used for supplementing the carbon, when the reducing sugar concentration is higher than 20g / L, the supplementing of the carbon is stopped, the rate for the supplementing of the carbon is adjusted according to real-time off-line determined reducing sugar concentration and fermentation liquid viscosity; amino nitrogen in the fermentation pot is controlled in 20-60mg / mL, when the amino nitrogen concentration is lower than 30mg / mL, the continuous flow adding method is used for supplementing the nitrogen, when the amino nitrogen concentration is higher than 60mg / mL, the supplementing of the nitrogen is stopped, and the rate for the supplementing of the nitrogen is adjusted according to real-time off-line determined amino nitrogen concentration and fermentation liquid viscosity. The method is simple in process, can obviously reduce the viscosity of the fermentation liquid and improve the oxygen transfer, and has an important effect on increasing of the yield of gentamicin.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, in particular relates to the fermentation production of gentamicin, in particular to a feeding method for reducing the viscosity of gentamicin C1a fermentation liquid. Background technique [0002] As an important aminoglycoside antibiotic, gentamicin C1a is the mother nucleus of a semi-synthetic new drug ictimicin, which is the first in China and has independent intellectual property rights. The traditional method of preparing C1a is to use resin separation technology to extract C1a single component from multi-component gentamycin. Due to the similar structure and similar physical and chemical properties of each component, the extraction cost remains high, and the high cost in the market The price of purity C1a remains high. [0003] Due to the many multi-component gentamicin fermentation processes, the current single-component gentamicin C1a fermentation process mainly draws o...

AI Technical Summary

Problems solved by technology

For example, conventional carbon supplementation uses a single component of glucose. In the literature reports on the carbon metabolism of gentamicin, high concentrations of glucose have a strong inhibitory effect on the synthesis of gentamicin, but although glucose has an inhibitory effect on the synthesis of antibiotics, But at the same time, it is also an essential carbon source for cell growth. The researchers tried to relieve the inhibition by adding the precursors of the gentamicin shunt metabolism. After trying, they found that although these substances can be effectively absorbed by the cells, they still cannot relieve the inhibition of glucose. Inhibition

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