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Method for detecting glutathione concentration in solution

A technology of glutathione and concentration, which is used in measuring devices, instruments, electrochemical variables of materials, etc., to achieve the effects of good stability, convenient operation and rapid detection

Active Publication Date: 2019-01-11
滁州市南方黑芝麻食品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are many methods for determining glutathione content, such as enzyme cycle method, colorimetry, fluorescence photometry, flow cytometry, titration, high performance capillary electrophoresis and high pressure liquid chromatography developed in recent years. etc. These methods have their own advantages, but they also have shortcomings. At present, there is no fast, stable, specific, sensitive and economical assay method. The glutathione assay method needs to be further developed and perfected.

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Embodiment 1

[0029] A method for detecting the concentration of glutathione in a solution, using a three-electrode system to detect glutathione in the solution to be tested by cyclic voltammetry, and obtaining the concentration of glutathione in the solution to be tested according to the linear equation of glutathione Concentration, the working electrode in the three-electrode system is modified by aminated graphene quantum dots, and the working electrode is a glassy carbon electrode.

[0030] Wherein, the method for detecting the concentration of glutathione in the solution, the specific operation of the modified treatment of aminated graphene quantum dots is as follows: the working electrode is immersed in a solution containing 2 mg / mL aminated graphene quantum dots, and scanned by cyclic voltammetry Then take it out and dry it in an infrared drying oven. The scanning parameters are: initial potential 0V, highest potential 1V, lowest point 0V, final potential 0V, scanning rate 0.1V / s, sca...

Embodiment 2

[0041] On the basis of Example 1, in the method for the concentration of glutathione in the described detection solution, the working electrode is first immersed in the electrolytic solution containing 10mM zirconium oxychloride and 0.3M potassium chloride with magnetized water preparation after pretreatment, Electrodeposit a layer of nano-zirconia layer, and then modify it with aminated graphene quantum dots. Finally, take 10 μL of dispersion liquid and drop-coat it on the working electrode electrodeposited with nano-zirconia layer. After drying, apply 7 μL of 0.03wt% Nafion Drop-coated on the surface of the electrode, dried for later use, the dispersion liquid is mixed with fumed silica, yeast dextran, propolis powder, and acetic acid with a volume fraction of 20% in a mass ratio of 15:1:1:500. It is prepared by ultrasonic dispersion, and the propolis powder is obtained by pulverizing propolis and passing through a 200-mesh sieve.

[0042] The method of embodiment 2 records ...

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Abstract

The invention discloses a method for detecting the glutathione concentration of a solution. According to the method, a three electrode system is used, cyclic voltammetry is adopted to measure the glutathione concentration of a solution to be measured, the glutathione concentration of the solution can be obtained through a glutathione linear equation; in the three electrode system, the work electrode is subjected to an aminated graphene quantum dot modification treatment, and the work electrode is a glass-carbon electrode. The method has the advantages of simple operation, quick detection, and high sensitivity, and is capable of high-sensitively recognizing glutathione in a mixed sample solution.

Description

technical field [0001] The invention relates to the technical field of glutathione detection. More specifically, the present invention relates to a method for detecting the concentration of glutathione in a solution by using cyclic voltammetry based on the electrochemical sensing modified by aminated graphene quantum dots. Background technique [0002] Glutathione is a tripeptide containing γ-amide bonds and sulfhydryl groups. It is composed of glutamic acid, glycine and cysteine. It is widely found in animals and plants and plays an important role in the physiological activities of organisms. There are two forms of glutathione, reduced and oxidized, and reduced glutathione exists in large quantities in the body and plays a major role. Glutathione is usually referred to as reduced glutathione. The molecular formula of glutathione is C 10 h 17 N 3 o 6 S is a colorless, transparent, slender crystal with a melting point of 189-193°C. In the structure of glutathione, ther...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/3277G01N27/3278G01N27/48
Inventor 肖琦黄珊冯萌萌卢双燕黄初升苏炜何英姿
Owner 滁州市南方黑芝麻食品有限公司
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