Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for identifying whether one or more chemicals of theophylline, caffeine and theobromine are added into traditional Chinese medicines for relieving cough and asthma

A technology for relieving cough, relieving asthma, and chemicals, applied in the field of medical analysis, can solve the problems of inability to meet rapid inspection, long detection time, expensive instruments, etc., and achieve the effects of high sensitivity, accurate results, and large enhancement effect.

Active Publication Date: 2017-03-22
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main methods for detecting whether theophylline, theobromine, caffeine and other drugs are added to Chinese medicine for relieving cough and asthma include HPLC-MS, GC-MS, etc. These methods have accurate detection results, but the disadvantages are expensive instruments and sample pretreatment. Complicated, the detection time is too long, and it cannot meet the requirements of on-site rapid inspection by the drug regulatory department

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for identifying whether one or more chemicals of theophylline, caffeine and theobromine are added into traditional Chinese medicines for relieving cough and asthma
  • Method for identifying whether one or more chemicals of theophylline, caffeine and theobromine are added into traditional Chinese medicines for relieving cough and asthma
  • Method for identifying whether one or more chemicals of theophylline, caffeine and theobromine are added into traditional Chinese medicines for relieving cough and asthma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The Raman spectrometer used in Example 1 is a BWS415-785H portable Raman spectrometer (Bida Tech, USA), with an excitation wavelength of 785nm. The surface enhancement agent used is nano-silver sol, and the preparation method is: take AgNO 3 Add 250 mL of secondary deionized water to dissolve 45 mg, and heat to a slight boil. Add 5 mL of 1% trisodium citrate aqueous solution dropwise, stir vigorously, and continue heating for 60 min. The gray-green colloid was obtained, cooled to room temperature, and set aside.

[0059] figure 1 For the flow chart of the method for identifying whether the Chinese medicine to be tested is mixed with theophylline, caffeine, or theobromine in this embodiment, it specifically includes the following four steps:

[0060] Step 1 (S1): Pre-adjustment of the pH of the solution to be tested

[0061] Add purified water as the extraction solvent to the Chinese medicine for relieving cough and asthma to be tested, conduct ultrasonic extraction...

Embodiment 2

[0105] In Example 2, four samples to be tested were respectively selected to analyze and identify Chinese medicines mixed with different concentrations of theophylline: No. 1 sample to be tested (containing 5% theophylline), No. 2 sample to be tested (containing 1% theophylline ), No. 3 sample to be tested (containing 0.05% theophylline). Below just according to the step that the method of the present invention proposes, No. 1, 2, 3 samples are analyzed and identified respectively. The percentages are mass fractions.

[0106] Sample No. 1 to be tested

[0107] According to the method described in step 1 to step 4 (S1-S4), the No. 1 test sample is analyzed and identified.

[0108] After steps 1 and 2, the one-dimensional dynamic surface-enhanced Raman spectrum of theophylline standard product is obtained, such as figure 2 shown. One-dimensional dynamic surface-enhanced Raman spectrum of caffeine standard, such as image 3 shown. One-dimensional dynamic surface-enhanced R...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of medicine analysis and specifically relates to a method for identifying whether one or more chemicals of theophylline, caffeine and theobromine are added into traditional Chinese medicines for relieving cough and asthma. The method comprises the following steps: pre-conditioning the pH value of a to-be-detected solution; collecting a one-dimensional dynamic surface-enhanced Raman spectrum chart; drawing a pH dependency-two-dimensional correlation surface-enhanced Raman spectrum chart; and identifying whether one or more chemicals of theophylline, caffeine and theobromine are added into a to-be-detected sample. The step of pre-conditioning the pH value in the method can guarantee that the spontaneous change in pH value occurs in the most sensitive scope of substances, a more distinctive dynamic change Raman spectrum chart can be acquired, the specificity is strong, the sensitivity is high, the operation steps are simple, the detection time is short, and whether the theophylline, caffeine or theobromine is added into the traditional Chinese medicines for relieving cough and asthma can be accurately identified.

Description

technical field [0001] The invention relates to the technical field of medical analysis, in particular to a method for identifying whether one or more of theophylline, caffeine, and theobromine are added to traditional Chinese medicines for relieving cough and asthma. Background technique [0002] In recent years, in order to speed up the curative effect of traditional Chinese medicine and seek high profits, some pharmaceutical companies have added one of theophylline, theobromine, and caffeine or a variety of methylpurine drugs to Chinese medicine for relieving cough and asthma. These medicines have the effects of strengthening the heart, diuresis, dilating coronary arteries, relaxing bronchial smooth muscle, and exciting the central nervous system, and can take effect quickly, so that the curative effect of traditional Chinese medicine for relieving cough and asthma can be accelerated. However, the safe range of blood concentration of these drugs is relatively narrow. Exce...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 陆峰武媚然柴逸峰黄超柳艳陈辉李皓吴棉棉张彬彬陈秀娟
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com