Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

In-vitro embryo culture solution containing Clusterin protein and application of in-vitro embryo culture solution in embryo cryopreservation

A technology of embryo culture medium and embryos, which is applied to the preservation and application of human or animal bodies, embryo cells, etc., and can solve the problems of antifreeze toxicity, decreased birth rate of fresh embryos, and inability to protect cells, etc.

Active Publication Date: 2017-03-22
金猪德元(北京)科技有限公司
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually, the antifreeze itself is toxic, too high a concentration will kill the cells before cooling down, and too low a concentration will not protect the cells
[0004] At the same time, there are also data in the existing technology that show that the birth rate of frozen embryos is much lower than that of fresh embryos during transplantation.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • In-vitro embryo culture solution containing Clusterin protein and application of in-vitro embryo culture solution in embryo cryopreservation
  • In-vitro embryo culture solution containing Clusterin protein and application of in-vitro embryo culture solution in embryo cryopreservation
  • In-vitro embryo culture solution containing Clusterin protein and application of in-vitro embryo culture solution in embryo cryopreservation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0044] 1 material

[0045] 1.1 Main instruments and equipment

[0046]

[0047] 1.2 Main reagents

[0048] 1.2.1 Recombinant Clusterin protein derived from mouse in vitro was purchased from Abcam (Product No.: ab194035), and its amino acid sequence is as follows:

[0049] EQEVSDNELQELSTQGSRYINKEIQNAVQGVKHIKTLIEKTNAERKSLLNSLEEAKKKKEDALEDTRDSEMKLKAFPEVCNETMMALWEECKPCLKHTCMKFYARVCRSGSGLVGQQLEEFLNQSSPFYFWMNGDRIDSLLESDRQQSQVLDAMQDSFARASGIIDTLFQDRFFARELHDPHYFSPIGFPHKRPHFLYPKSRLVRSLMSPSHYGPPSFHNMFQPFFEMIHQAQQAMDVQLHSPAFQFPDVDFLREGEDDRTVCKEIRRNSTGCLKMKGQCEKCQEILSVDCSTNNPAQANLRQELNDSLQVAERLTEQYKELLQSFQSKMLNTSSLLEQLNDQFNWVSQLANLTQGEDKYYLRVSTVTTHSSDSEVPSRVTEVVVKLFDSDPITVVLPEEVSKDNPKFMDTVAEKALQEYRRKSRAEVDHHHHHH

[0050] 1.2.2 CZB medium

[0051] (1) CZB stock solution 100mL formula

[0052]

[0053] Preparation method: Mix liquid A and liquid B, and then dilute to 100mL with high-pressure ultra-pure water.

[0054] (2) CZB working solution 5mL formula

[0055]

[0056]

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of embryo cryopreservation and particularly relates to an in-vitro embryo culture solution for increasing the embryo unfreezing thawing rate. The in-vitro embryo culture solution comprises Clusterin protein. The invention further relates to a method for improving embryo freezing resistance and increasing the unfreezing thawing rate and a method for freezing an embryo. By means of the in-vitro embryo culture solution and the methods, the freezing resistance of the embryo can be remarkably improved, and the unfreezing thawing rate of the embryo can be remarkably increased.

Description

technical field [0001] The invention belongs to the field of cryopreservation of embryos. Specifically, the present invention relates to the application of Clusterin protein in cryopreservation of embryos. Background technique [0002] In embryo biotechnology such as embryo transfer, in vitro fertilization, transgenic and cloning, cryopreservation of embryos is often involved, and how to better freeze embryos so that they can still develop normally after thawing has always attracted much attention . [0003] Anticryoprotectants are often employed in current embryo freezing methods. Anti-cryoprotectants are mainly used to prevent cells from being damaged during freezing. Antifreeze protective agents are mostly permeable small molecular substances, which mainly penetrate into the interior of cells and undergo hydration to increase the viscosity of the intracellular solution, thereby protecting cells from freezing damage. Different cryoprotectants have different effects on ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073A01N1/02
CPCA01N1/0221C12N5/0604C12N2517/10
Inventor 郭勇倪和民陈余齐晓龙翟椿东刘迪
Owner 金猪德元(北京)科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com