Preparation method of psophocarpus tetragonolobus polysaccharides and application of psophocarpus tetragonolobus polysaccharides in preparation of anti-aging cosmetics
A square-edge bean and anti-aging technology, applied in the preparation of anti-aging cosmetics, the field of preparation of square-lens bean polysaccharides, can solve the problems of anti-aging cosmetics such as good effect, unsatisfactory effect, and low price, and achieve the effect of inhibiting bacteria Prosperity, anti-aging effect is good, the effect of low price
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Embodiment 1
[0031] Take 1000g of square beans and clean them, microwave dry to constant weight; crush the dried square beans to 20 mesh; add deionized water to the crushed square beans powder, the ratio of solid to liquid is 1:10, ultrasonic extraction 40min, extract once; centrifuge to separate the supernatant, concentrate under reduced pressure to 1 / 10 of the original volume for use; weigh 10U / g of Bacillus β-amylase, add deionized water, dissolve completely, and prepare a concentration of 5U / mL amylase solution; add the amylase solution to the concentrated extract in step (4), stir evenly, and adjust the pH of the solution to 5.0; heat in a water bath, keep the solution temperature at 40°C, and enzymolyze for 4-6h; After hydrolysis, the temperature was raised to 75°C, and the enzyme was inactivated for 10 minutes; the supernatant was separated by centrifugation; the Sevage method was combined with the enzyme method to remove the protein, and concentrated to 1 / 10 of the original volume ...
Embodiment 2
[0033] Take 1000g of square beans and clean them, microwave dry to constant weight; crush the dried square beans to 28 mesh; add deionized water to the crushed square beans powder, the ratio of solid to liquid is 1:14, ultrasonic extraction 40min, extract once; centrifuge to separate the supernatant, concentrate under reduced pressure to 1 / 10 of the original volume for use; weigh 10U / g of Bacillus β-amylase, add deionized water, dissolve completely, and prepare a concentration of 5U / mL of amylase solution; add the amylase solution to the concentrated extract in step (4), stir evenly, and adjust the pH of the solution to 6.0; heat in a water bath, keep the solution temperature at 48°C, and enzymolyze for 4-6h; After hydrolysis, the temperature was raised to 75°C, and the enzyme was inactivated for 10 minutes; the supernatant was separated by centrifugation; the Sevage method was combined with the enzyme method to remove the protein, and concentrated to 1 / 10 of the original volu...
Embodiment 3
[0035] Take 1000g of square beans and clean them, microwave dry to constant weight; crush the dried square beans to 24 mesh; add deionized water to the crushed square beans powder, the ratio of solid to liquid is 1:12, ultrasonic extraction 40min, extract once; centrifuge to separate the supernatant, concentrate under reduced pressure to 1 / 10 of the original volume for use; weigh 10U / g of Bacillus β-amylase, add deionized water, dissolve completely, and prepare a concentration of 5U / mL amylase solution; add the amylase solution to the concentrated extract in step (4), stir evenly, and adjust the pH of the solution to 5.3; heat in a water bath, keep the solution temperature at 46°C, and enzymolyze for 4-6h; After hydrolysis, the temperature was raised to 75°C, and the enzyme was inactivated for 10 minutes; the supernatant was separated by centrifugation; the Sevage method was combined with the enzyme method to remove the protein, and concentrated to 1 / 10 of the original volume ...
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