Rubus-chingii seed breeding method
A technology of palm leaf raspberry and seeds, applied in the field of breeding, can solve the problems of low reproduction coefficient, difficult reproduction, irregular germination, etc., and achieve the effect of overcoming low reproduction coefficient, large reproduction coefficient and easy operation
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Embodiment 1
[0059] The method for described palm leaf raspberry seed propagation comprises the steps:
[0060] (1) Pretreatment: Pick ripe raspberry fruits with palm leaves, remove sepals and fruit stalks, leave spherical aggregated achenes, crush them, rinse off the pulp with clean water, and dry them at a temperature of 23-25°C and a humidity of 20°C Save at -50%;
[0061] (2) Acid etching: Mix 98% concentrated sulfuric acid with the pretreated seeds, soak for 25 minutes, rinse with water for 10 minutes, and gently rub off the carbonized part;
[0062] (3) Potassium permanganate treatment: the seeds after the acid etching are mixed with a mass fraction of 0.2% potassium permanganate solution, soaked for 10 minutes, rinsed 3-5 times with sterile water, and dried;
[0063] (4) Gibberellin treatment: soak the seeds treated with potassium permanganate in a mixed solution of 2 mg / L gibberellin and 34 mg / L potassium nitrate for 24-36 hours;
[0064] (5) Low-temperature sand storage: bury th...
Embodiment 2
[0068] The method for described palm leaf raspberry seed propagation comprises the steps:
[0069] (1) Pretreatment: Pick ripe palmate raspberry fruits, remove sepals and fruit stalks, leave spherical aggregated achenes, crush them, rinse off the pulp with clean water, and dry them at a temperature of 20-23°C and a humidity of 35°C. Save at -60%;
[0070] (2) Acid etching: Mix 75% concentrated sulfuric acid with the pretreated seeds, soak for 15 minutes, rinse with water for 8 minutes, and gently rub off the carbonized part;
[0071] (3) Potassium permanganate treatment: mix the seeds after acid etching with a mass fraction of 0.1% potassium permanganate solution, rinse them twice with sterile water after soaking for 20 minutes, and dry them;
[0072] (4) Gibberellin treatment: soak the seeds treated with potassium permanganate in a mixed solution of 1 mg / L gibberellin and 20 mg / L potassium nitrate for 45 hours;
[0073] (5) Low-temperature sand storage: bury the seeds treat...
Embodiment 3
[0077] The method for described palm leaf raspberry seed propagation comprises the steps:
[0078] (1) Pretreatment: Pick ripe raspberry fruits with palm leaves, remove sepals and fruit stalks, leave spherical aggregated achenes, crush them, rinse off the pulp with clean water, and dry them at a temperature of 26-30°C and a humidity of 15°C. Save at -40%;
[0079] (2) Acid etching: Mix and stir 85% concentrated sulfuric acid with the pretreated seeds, soak for 35 minutes, rinse with water for 30 minutes, and gently rub off the carbonized part;
[0080] (3) Potassium permanganate treatment: mix the seeds after acid etching with a mass fraction of 0.8% potassium permanganate solution, rinse with sterile water 10 times after soaking for 6 minutes, and dry;
[0081] (4) Gibberellin treatment: soak the seeds treated with potassium permanganate in a mixed solution of 10 mg / L gibberellin and 40 mg / L potassium nitrate for 15 hours;
[0082] (5) Low-temperature sand storage: bury the...
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