Method based on genome LoF loci to screening indirectly related kiwi fruit varietal character

Abstract

The invention discloses a method based on genome LoF loci to screening indirectly related kiwi fruit varietal character. The method includes: acquiring a variety genome sequence; comparing with a reference genome of the sequence, discovering and classifying mutation; screening and acquiring LoF mutation loci, and dividing types; performing functional annotation and classification on the LoF mutation loci; screening LoF mutation information unique to varieties, and performing varietal character indirect relation analysis. Compared with previous methods of direct artificial observation on varietal character of kiwi fruit variety and seed breeding experiment detection, the method has higher stability and repeatability, so that relation prediction on the varietal character of the variety is more accurate; by using the method, potential varietal character feature and breedable utilization value of a specific kiwi fruit variety can be quickly acquired and predicted, so that efficiency in resource evaluation and breeding application is improved greatly.

Description

technical field [0001] The invention belongs to the field of plant genetic resource evaluation and biotechnology, and specifically relates to a method for screening indirectly associated kiwifruit species based on genomic LoF sites and its breeding application, that is, the molecular genetic basis for stably inherited kiwifruit species characteristics Analysis and identification, in order to improve the utilization efficiency of kiwifruit resource evaluation and genetic breeding, and promote the rapid cultivation of new varieties. Background technique [0002] my country is rich in wild resources of Actinidia Lindl., and among the 75 taxa of about 54 species in this genus, the natural distribution of most taxa is unique to my country. How to make full use of abundant natural resources, especially wild relatives of cultivated kiwifruit with various shapes, is an important research content of current kiwifruit genetics and breeding work. In the process of evaluating and utili...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of difficult identification and utilization of kiwifruit species characteristics that can be stably inherited, and to provide a method for screening indirectly related kiwifruit species based on genomic LoF sites. The method starts with genomic LoF variation sites and screens Check the specific LoF variation site information of kiwifruit species association and perform relevant functional annotations, so as to indirectly correlate the variation attributes and range of species germline characteristics, and finally form resource evaluation and breeding applications

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