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Method for extracting high purity stachyose from Stachys floridana schuttl. Ex benth

A stachyose, high-purity technology, applied in the field of comprehensive utilization of agricultural products, can solve the problems of not considering the retention of stachyose activity in silver bars, reducing the yield of stachyose, not obtaining deproteinization and the like, and achieving a simple implementation process. Convenience, low cost and the effect of reducing the loss rate

Active Publication Date: 2017-03-15
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In addition, the purification of stachyose in silver bars also has the following problems: 1. There is no effective deproteinization method, and the general deproteinization process is often accompanied by the loss of oligosaccharides
2. The stachyose was not effectively released from the silver bars, which reduced the yield of stachyose
3. The retention of stachyose activity in silver bars is not considered

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Preparation of dry silver bars: using silver bars from Yanshi, Henan Province as raw materials, fresh silver bars were picked and washed, then dried at 60°C for 10 hours to obtain dry silver bars, and then ultrafinely pulverized to finally obtain dry silver bars.

[0033] 2. Enzymolysis: Weigh 1000.0g of silver bar dry powder, use papain and plant compound enzyme (volume ratio 1:1) for enzymolysis treatment, the ratio of solid to liquid is 1:30g / ml, and the amount of enzyme added is the mass of the mixed material liquid 0.3% of 0.3%, enzymolysis time is 2h, enzymolysis temperature is 50°C, after enzymolysis, centrifuge to obtain silver bar enzymolysis treatment solution;

[0034] 3. Preparation of stachyose extract: add ethanol with an equal volume concentration of 85% to the enzymolysis treatment solution, mix evenly, transfer to a rotary evaporator, then reflux at 45°C for 95 minutes, and vacuum pump to obtain the extract , the extract was filtered through a 0.45 μ...

Embodiment 2

[0041] 1. Preparation of dry silver bars: After fresh silver bars are picked and washed, dry them at 60°C for 10 hours to obtain dry silver bars, and then perform ultrafine grinding to finally obtain dry silver bars.

[0042] 2. Enzymolysis: Weigh 1000.0g of silver bar dry powder, use papain and plant compound enzyme (volume ratio 1:1) to carry out enzymolysis treatment, the ratio of solid to liquid is 1:30g / ml, and the amount of enzyme added is 0.3%. The hydrolysis time is 3 hours, and the enzymolysis temperature is 45°C. After enzymolysis, centrifuge to obtain silver bar enzymatic hydrolysis treatment solution;

[0043] 3. Preparation of stachyose extract: add an equal volume of ethanol with a concentration of 85% to the enzymolysis treatment solution, mix well and transfer to a rotary evaporator, then reflux at 45°C for 95 minutes, and vacuum pump to extract The extract was filtered through a 0.45 μm microporous membrane. The purity of stachyose obtained in this step is: 5...

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Abstract

The invention discloses a method for extracting high purity stachyose from Stachys floridana schuttl. Ex benth and belongs to the technical field of comprehensive utilization of agricultural products. The method comprises cleaning fresh Stachys floridana schuttl. Ex benth as a raw material from the Yanshi city in the Henan province, drying the raw material, carrying out ultrafine pulverization, carrying out complex enzymolysis pretreatment, carrying out alcohol extraction to obtain stachyose crude extract, wherein an extraction rate of stachyose of Stachys floridana schuttl. Ex benth is more than 45%, carrying out dialysis to remove proteins in the stachyose crude extract, carrying out clarification by diatomite, carrying out decolorization and desalination through activated carbon, carrying out centrifugation, then carrying out concentration, carrying out freeze drying to obtain a stachyose crude product, carrying out DEAE fiber column chromatography, then carrying out concentration, and carrying out freeze drying to obtain stachyose with purity of 95 to 99.9%. The method for extracting stachyose is simple and the production cost is low. The method can create conditions for the development of stachyose health foods.

Description

technical field [0001] The invention relates to a method for extracting high-purity stachyose from silver bars, and belongs to the technical field of comprehensive utilization of agricultural products. Background technique [0002] Stachyose is a naturally occurring non-reducing tetrasaccharide, which belongs to the galactoside oligosaccharides of the genus Raffinose. Its physiological functions mainly include: providing lower energy, preventing dental caries, improving the internal environment of the human digestive tract, regulating the intestinal microecological balance, and benefiting the health of the host. Toxicological experiments have confirmed that stachyose is safe and reliable, without any toxic and side effects. Under normal conditions, stachyose does not chemically react with other chemical substances (such as food additives, etc.), and does not affect the smell and taste of other substances. It is an excellent functional food base material and was once used as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/06C07H1/08
CPCC07H1/08C07H3/06
Inventor 钟先锋黄桂东白永亮曾荣郭衍彪曹诗琳
Owner FOSHAN UNIVERSITY
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