Fast preparation method of silk fibroin-hyaluronic acid complex gel

Abstract

The invention relates to a fast preparation method of silk fibroin-hyaluronic acid complex gel. The fast preparation method comprises the following steps: dissolving silk fibroin obtained by degumming, preparing a regenerated silk fibroin solution through dialysis, mixing the regenerated silk fibroin solution with hyaluronic acid, and finally preparing the fast complex gel under the action of a crosslinking agent. Compared with a conventional hyaluronic acid gel formed by pure crosslinking, the complex gel can be fast formed and has the advantages that by adding the silk fibroin, the gelating time is effectively shortened, the degrading speed of the gel is reduced, the stay time for tissue repairing and filling is prolonged, and the operability is improved; compared with pure silk fibroin hydrogel, the complex gel has the advantage that by adding the hyaluronic acid, the shortcomings of low toughness, fragility and the like of the silk fibroin hydrogel are overcome. The method for preparing the complex gel is simple in process and short in gelating time; a novel method is provided for the preparation of functional complex gel; the prepared complex gel can be used in the field of medicinal health, tissue engineering, environment protection and the like.

Description

technical field [0001] The invention belongs to a processing technology of a regenerated silk fibroin solution, specifically, a composite gel material with high mechanical properties and water absorption properties and a preparation method thereof, which can be used as a drug release carrier, a tissue engineering support, etc. for biological applications. medical field. Background technique [0002] Gel is a kind of spatial network structure between solid and liquid. It is soft in nature, can maintain a certain shape, and can retain a large amount of water. Due to the physical and chemical crosslinking between polymer chains, the gel is insoluble in water, but can swell and maintain a certain shape. Its properties are similar to those of the extracellular matrix. After absorbing water, it can reduce friction and mechanical effects on surrounding tissues, and can improve the biological properties of the material. Due to its unique water absorption, water retention and biomi...

AI Technical Summary

Problems solved by technology

However, the composite gel only relies on silk fibroin, or the physical interaction between silk fibroin and hyaluronic acid, and the mechanical properties need to be improved. Furthermore, the content of hyaluronic acid is less, and the water absorption of the composite gel is poor.