O type foot and mouth disease virus-like particle and preparation method thereof and application

Abstract

The invention discloses an O type foot and mouth disease virus-like particle and a preparation method of the O type foot and mouth disease virus-like particle and an application. The O type foot and mouth disease virus-like particle is formed by assembling structural protein VP0, VP1 and optimized OP3 structural proteins of O type foot and mouth disease virus, wherein the gene sequence of the VP1 is shown as SEQ ID NO.1; the gene sequence of the VP0 is shown as SEQ ID NO.2; the gene sequence of the optimized VP3 is shown as SEQ ID NO.3. The invention tries to perform artificially missing on a part of section of the structural protein VP3 of O type foot and mouth disease virus; the result shows that the protein expression amount of the VP3 gene after the artificial missing is improved by 20% in comparison to that before mutation; the assembling efficiency of the virus-like particle is also improved by 15%; moreover, the animal test result shows that the immunogenicity thereof is good and free from significant difference with VLPs obtained through unmissed VP3 assembling. The invention provides a new technical manner for the research of the foot and mouth disease vaccine.

Description

technical field [0001] The invention relates to an O-type foot-and-mouth disease virus-like particle, a preparation method and application thereof, and belongs to the technical field of agricultural science, animal husbandry and veterinary medicine. Background technique [0002] The outbreak of animal viral diseases often seriously affects the production and export trade of animal products in the whole country. For example, foot-and-mouth disease virus, which causes severe infectious diseases of cloven-hoofed animals, is listed as the first class A infectious disease by the World Organization for Animal Health (OIE). In the long-term development plan of national animal disease prevention and control, it is determined as the first disease species to be prevented and controlled by immune purification within a limited period. At present, traditional vaccines such as whole-virus inactivated vaccines are mainly used at home and abroad to prevent and control viral infectious disea...

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Problems solved by technology

[0004] Although the research on VLPs has been reported successively since the 1950s and 1960s, most of the research on VLPs has been concentrated on the eukaryotic expression system, especially the baculovirus / insect expression system. VLPs vaccines, but due to factors such as high production costs and low VLPs yield of this system, the industrialization process of animal virus VLPs vaccines has been limited, and commercial animal virus VLPs vaccines have not yet appeared.

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