Lactobacillus zeae LZ3 and application thereof in aquaculture
A technology of Lactobacillus maize and strains is applied in the field of fishery ecological safety, which can solve the problems of water body hypoxia, destroying water body ecological balance, affecting photosynthesis of aquatic plants, etc., so as to improve water quality, prevent harm, and eliminate ammonia nitrogen and nitrite. Effect
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Embodiment 1
[0040] Embodiment 1 Aeromonas hydrophila antagonistic bacteria search, screening, identification
[0041] (1) Search and screening of antagonistic bacteria against Aeromonas hydrophila
[0042] A) material
[0043] Aeromonas hydrophila strain: The strain was isolated from the blood of crucian carp suffering from bacterial sepsis, and its pathogenicity was confirmed by the return test of healthy crucian carp; it was identified as Aeromonas hydrophila by the BBL Crystl microbial automatic analysis system bacteria (Aeromonas hydrophila).
[0044] Bottom mud: The bottom mud collected from the Wangwei breeding area of Taihu Lake.
[0045] Medium: trypticase soy agar (TSA) medium, nutrient broth (NB) medium, purchased from reagent companies.
[0046] Preparation of TSA medium: Weigh 42g of TSA, dissolve it in a Erlenmeyer flask of 1000ml distilled water, stir to dissolve, sterilize by high-pressure steam at 121°C for 15min, pour it into a petri dish, and store it at 4°C for later...
Embodiment 2
[0058] Trial production of embodiment 2 antagonistic bacteria preparation (fermentation agent)
[0059] According to the biological characteristics of Lactobacillus zea, a suitable medium was screened out, and appropriate fermentation conditions were adopted to make Lactobacillus zea LZ3 into a fermentation broth, and a certain concentration of Aeromonas hydrophila was prepared by adding an appropriate amount of stabilizer. Bacteria antagonistic antimicrobial starter.
[0060] Concretely adopted fermentation conditions are as follows:
[0061] Fermentation strain: Lactobacillus zea LZ3.
[0062] Fermentation medium formula: peptone 10-80 grams, sucrose 20-100 grams, yeast powder 1-10 grams, purified water 1000 ml.
[0063] Fermentation temperature: 28-30°C.
[0064] Fermentation period: 64-70 hours.
[0065] Rotational speed of fermentation shaker: 150-200r / min.
[0066] Stabilizer: 5-10 grams of Tween-20 and 10-20 grams of glycerin per liter of fermentation broth.
[00...
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