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Skin repair material with biological activity and method for preparing skin repair material

A technology for skin repair and bioactivity, which is applied in the fields of biomedical engineering and skin repair material preparation, which can solve the problems of low accuracy of cell implantation, insufficient accuracy of scaffold structure, time-consuming preparation process, etc., and achieve abundant raw materials and low price , The effect of simple preparation method

Inactive Publication Date: 2017-02-22
GUANGDONG TAIBAO MEDICAL SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to address the defects of the existing tissue engineered skin, and solve the shortcomings of the existing tissue engineered skin, such as insufficient accuracy of the scaffold structure, low accuracy of cell implantation, and time-consuming preparation process.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A biologically active skin repair material is a two-layer structure consisting of a simulated epidermis layer and a simulated skin layer; the simulated epidermis layer consists of 10 parts by weight of collagen, 10 parts of DMEM medium and 0.01 part of keratinocytes , the artificial cortex is composed of 10 parts of collagen, 10 parts of DMEM medium and 0.01 part of fibroblast by weight parts. The collagen is a commercially available porcine-derived collagen, and the DMEM medium is a commercially available medium for cell culture. The keratinocytes and fibroblasts are obtained from human skin and cultured in DMEM medium with 10% bovine serum albumin by volume.

Embodiment 2

[0031] A biologically active skin repair material is a two-layer structure consisting of a simulated epidermis layer and a simulated skin layer; the simulated epidermis layer consists of 10 parts by weight of collagen, 10 parts of DMEM medium and 0.02 parts of keratinocytes , the artificial cortex is composed of 10 parts of collagen, 10 parts of DMEM medium and 0.02 part of fibroblast by weight parts. The collagen is a commercially available porcine-derived collagen, and the DMEM medium is a commercially available medium for cell culture. The keratinocytes and fibroblasts are obtained from human skin and cultured in DMEM medium with 10% bovine serum albumin by volume.

Embodiment 3

[0033] A biologically active skin repair material is a two-layer structure consisting of a simulated epidermis layer and a simulated skin layer; the simulated epidermis layer consists of 10 parts by weight of collagen, 10 parts of DMEM medium and 0.03 parts of keratinocytes , the artificial cortex consists of 10 parts of collagen, 10 parts of DMEM medium and 0.03 parts of fibroblasts by weight. The collagen is a commercially available porcine-derived collagen, and the DMEM medium is a commercially available medium for cell culture. The keratinocytes and fibroblasts are obtained from human skin and cultured in DMEM medium with 10% bovine serum albumin by volume.

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Abstract

The invention belongs to the technical field of biomedical engineering, particularly belongs to the field of technologies for preparing skin repair materials, and discloses a skin repair material with biological activity and a method for preparing the skin repair material. The skin repair material comprises collagen, DMEM (Dulbecco modified eagle media), keratinocytes and fibroblasts. The skin repair material with the biological activity and the method have the advantages that the skin repair material is low in immunogenicity and excellent in biocompatibility, materials for the skin repair materials come from abundant sources, and obvious effects of promoting wound healing can be realized; the skin repair material is prepared from collagen solution with biological activity by the aid of biological three-dimensional printing technologies and can be prepared according to actual conditions of patients, integral preparation procedures are short in consumed time, the skin repair material can be immediately prepared and used, accordingly, the patients can be truly individually treated, and influence on the performance of products due to misoperation during product transportation or storage can be prevented.

Description

technical field [0001] The invention belongs to the technical field of biomedical engineering, in particular to the technical field of preparation of skin repair materials, and specifically relates to a biologically active skin repair material and a preparation method thereof. Background technique [0002] Skin damage caused by mechanical, chemical corrosion or microbial infection is a common skin disease every day. Although most skin wounds can heal naturally, large-scale injuries or skin injuries caused by acute and chronic patients are difficult to heal without proper treatment. The traditional treatment method is to repair the injured wound by autologous or allogeneic skin donor, but this treatment method has the shortage of limited donors. Tissue-engineered skin is prepared using the mode of "scaffold + seed cells", that is, a scaffold material with a porous structure is prepared first, and then seed cells are seeded on the scaffold for cultivation, and finally tissue-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/44A61L27/38
CPCA61L27/60A61L27/3804A61L27/3813A61L27/44C08L89/06
Inventor 卢亢陈锦涛陈泰瀛
Owner GUANGDONG TAIBAO MEDICAL SCI TECH
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